Abstract
Schizophrenia is a devastating illness that affects up to 1% of the population; it is characterized by a combination of positive symptoms, negative symptoms, and cognitive impairment. Currently, treatment consists of one class of medications known as antipsychotics, which include typical (first-generation) and atypical (second-generation) agents. Unfortunately, antipsychotic medications have limited efficacy, with up to a third of patients lacking a full response. Clozapine, the first atypical antipsychotic developed, is the only medication shown to be superior to all other antipsychotics. However, owing to several life-threatening side effects and required enrollment in a registry with routine blood monitoring, clozapine is greatly underutilized in the US. Developing a medication as efficacious as clozapine with limited side effects would likely become the first-line therapy for schizophrenia and related disorders. In this review, we discuss the history of clozapine, landmark studies, and its clinical advantages and disadvantages. We further discuss the hypotheses for clozapine’s superior efficacy based on neuroreceptor binding, and the limitations of a receptor-based approach to antipsychotic development. We highlight some of the advances from pharmacogenetic studies on clozapine and then focus on studies of clozapine using unbiased approaches such as pharmacogenomics and gene expression profiling. Finally, we examine how these approaches could provide insights into clozapine’s mechanism of action and side-effect profile, and lead to novel and improved therapeutics.
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Introduction
Schizophrenia is a devastating illness that affects up to 1% of the population [1, 2], characterized by a combination of positive symptoms, negative symptoms, and cognitive impairment. Positive symptoms are abnormal mental experiences that include hallucinations, delusions, and thought disorder [3]. Negative symptoms relate to loss of mental energy and efficiency, such as limited emotional expression, apathy, social withdrawal, and poverty of thought and speech [3]. The cognitive decline associated with schizophrenia does not lead to gross dementia; it mainly affects executive functioning, verbal memory, and attention [3, 4]. The symptoms of schizophrenia can be tormenting to patients, greatly affecting their quality of life. In addition, lifetime suicide risk is 5% [5, 6], mortality rate is 2 to 3 times higher than that of the general population [7], and life expectancy is 20% shorter [8].
Schizophrenia cost the US $155.7 billion in 2013, including $9 billion in medication expenses [9]. Currently, treatment consists of one class of medications, known as antipsychotics that include typical (first-generation) and atypical (second-generation) agents. Despite their cost, antipsychotic medications have limited efficacy, with up to a third of patients lacking a full response [10, 11]. Clozapine is the only medication indicated for treatment refractory schizophrenia, usually defined as the failure of 2 other antipsychotics for any reason. Up to 70% of refractory patients demonstrate improvement in their symptoms on clozapine, but the remaining 30% of patients tend to be refractory to all medications [12]. As we will describe in this review, the field desperately needs medications with improved efficacy and limited side effects.
Development of First-Generation or Typical Antipsychotics
The first antipsychotic identified was chlorpromazine, originally synthesized as an anesthetic adjunct. The French surgeon Henri-Marie Laborit noticed its unusual calming properties [13, 14], and later psychiatrist Heinz Lehmann performed clinical trials with chlorpromazine that led to US Food and Drug Administration (FDA) approval in 1954 [15]. The development of other typical antipsychotics in the late 1950s to mid-1970s were based on chemical structure and an agent’s ability to cause catalepsy in rats [15, 16]. Unfortunately, in many cases, these typical antipsychotics caused severe and debilitating movement disorders, including dystonia and tardive dyskinesia. This created a need for effective antipsychotics that do not cause movement disorders.
Development of Clozapine and its Superior Efficacy
Clozapine, the first atypical antipsychotic developed [15], has been termed atypical because, in contrast to typical antipsychotics, it does not produce significant extrapyramidal side effects, does not elevate prolactin levels, and does not induce tardive dyskinesia after long-term use [17, 18]. In preclinical studies clozapine appeared to be effective without causing catalepsy, which was surprising since at the time catalepsy was considered necessary for efficacy [13]. Unfortunately, as testing on clozapine was just beginning in the US, 16 patients in Finland developed agranulocytosis leading to 8 deaths, and clozapine was removed from the market in countries where it had been introduced [13, 15].
As the use of typical antipsychotics continued to increase, the number of patients that developed severe movement disorders also rose. This unfortunate situation renewed interest in clozapine, owing to its ability to treat schizophrenia with minimal risk of causing movement disorders, and led the FDA to consider approving clozapine only if it had superior efficacy to standard antipsychotic medications [15]. Therefore, in 1988, Kane et al. [19] conducted a multicenter clinical trial with 286 patients who failed to respond to haloperidol after 6 weeks, who were then randomly assigned to either clozapine or chlorpromazine for 6 weeks. This landmark study showed that 30% of the haloperidol nonresponders improved on clozapine in the Brief Psychiatric Rating Scale and the Clinical Global Impression Scale versus 4% on chlorpromazine. Clozapine also resulted in a greater improvement in negative symptoms. This study was important because it showed that clozapine was superior to another antipsychotic, and that clozapine could successfully treat refractory schizophrenia. Following this study, clozapine received approval for refractory schizophrenia in the US. However, the FDA required that all patients on clozapine enroll in a registry with routine blood monitoring for agranulocytosis.
Two pivotal prospective studies substantiated clozapine’s superiority among atypical antipsychotics. The first trial, the Clinical Antipsychotic Trials for Interventions Effectiveness (CATIE) phase II investigation, studied patients from the CATIE phase I trial that did not respond to 1 of the 4 atypical antipsychotics used originally (risperidone, quetiapine, ziprasidone, and olanzapine) [20]. Patients were then randomized to either clozapine or 1 of 3 atypical antipsychotics that they were not previously on (olanzapine, quetiapine, or risperidone). The main outcome was discontinuation of medication for any reason, as this best represents real-world prescribing practices. The study showed superior results for time to discontinuation with clozapine (median time of 10.5 months) versus quetiapine (median time of 3.3 months), risperidone (median time of 2.8 months), and olanzapine (median time of 2.7 months). Furthermore, the study demonstrated less discontinuation on clozapine, with 44% of the patients completing the 18-month study versus 18% for the other medications. In addition, at 3 months, the patients on clozapine showed significant improvement in their Positive and Negative Syndrome Scale (PANSS) total scores compared with patients treated with quetiapine or risperidone (but not olanzapine).
The second study, performed in the UK, and termed the Cost Utility of the Latest Antipsychotic Drugs in Schizophrenia Study (CUtLASS 2) [21], attempted to determine if clozapine was more efficacious than other antipsychotics in its class. Patients with schizophrenia or related disorders who had failed at least 2 other antipsychotics owing to poor response were randomized to clozapine or either risperidone, olanzapine, quetiapine, or amisulpride. At 1 year follow-up, patients on clozapine had a significant improvement in PANSS total score and a trend towards improvement in quality-of-life score compared with the other antipsychotics. In addition, at 3 months, patients on clozapine reported greater improvement in their overall mental health than patients on other antipsychotics in its class.
Meta-analyses of antipsychotic medications have consistently demonstrated that clozapine is superior to all other antipsychotics [22]. However, standard meta-analysis cannot provide a hierarchy of antipsychotic efficacy. Therefore, Leucht et al. utilized a Bayesian-framework, multiple-treatments meta-analysis of randomized controlled trials to compare antipsychotics and placebo in the acute treatment of schizophrenia [23]. They identified 212 studies that met their inclusion criteria with a total of 43,049 participants, and determined that clozapine was significantly more effective than the 14 other antipsychotics included (amisulpride, olanzapine, risperidone, paliperidone, zotepine, haloperidol, quetiapine, aripiprazole, sertindole, ziprasidone, chlorpromazine, asenapine, lurasidone, and iloperidone). In fact, it was the only medication available in the US to separate from all other antipsychotics in efficacy (Fig. 1).
Efficacy of antipsychotic medications. Adapted from Leucht et al. [23]
Furthermore, clozapine is the only medication to have an FDA indication for reducing the risk of recurrent suicidal behavior in patients with schizophrenia or schizoaffective disorder [24]. Clozapine received this indication after an international multicenter randomized study, conducted over 2 years, demonstrated a 25% improvement over olanzapine for all measures of suicidality (reduction in suicide attempts, hospitalization, and a scale for quantifying suicidality). In addition, clozapine has been shown to reduce violence [25].
Disadvantages of Clozapine
Clozapine’s Side-Effect Profile
Despite clozapine’s superior efficacy, it has several life-threatening side effects. Agranulocytosis (now called severe neutropenia) occurs when the absolute neutrophil count is < 500/μl. The risks are estimated to be 0.8% for severe neutropenia and 3% for neutropenia [26]. As agranulocytosis or severe neutropenia can lead to death, the FDA instituted the clozapine Risk Evaluation and Mitigation Strategies (REMS) registry. All patients on clozapine must enter this registry with weekly blood monitoring of their absolute neutrophil count for the first 6 months, every 2 weeks for the next 6 months, and then monthly for as long as the patient is on clozapine. The establishment of a national registry has dramatically reduced the expected deaths from clozapine [27].
Myocarditis occurs in 1 out of 10,000 to 1 out of 500, with a mortality rate of up to 50% [28]. Approximately 80% of the incidents of myocarditis occur within the first month and 90% by 2 months. Clozapine can also cause venous thromboembolism, with an estimated mortality rate of 44% [29]. Close following of electrocardiograms, troponins, and C-reactive protein during the first 2 months can mitigate cardiovascular events.
The risk of developing seizures can be up to 10% after 3.8 years [30, 31]. However, seizures arising from clozapine treatment are not a contraindication and clozapine can be continued with the co-administration of an antiseizure medication. Gastrointestinal hypomotility occurs in approximately 14% of patients with a mortality rate as high as 27.5% [32]. A mean weight gain of 30 lb was observed in a 10-year cohort, with most weight gain occurring during the first 6 to 12 months. Finally, clozapine is also associated with a high risk of developing diabetes mellitus [33]. Unfortunately, gastrointestinal hypomotility and metabolic disturbances remain problematic despite monitoring.
Clozapine is Underutilized
Clozapine has been underutilized in the US, owing to its life-threatening side effects, the difficulty in initiating the medication safely, the need for regular blood monitoring, and the often disorganized nature of patients requiring clozapine [10, 22]. Furthermore, this has led to the use of high doses of other antipsychotics, polypharmacy with multiple antipsychotics, or high doses of multiple antipsychotics, which greatly increase the likelihood of adverse events and still only lead to limited improvement in clinical symptoms [10]. Collectively, up to 40% of all patients with schizophrenia and related disorders could benefit from clozapine [10, 34]. However, in the US only 4.4% of patients were on clozapine in 2008 [34], with an average delay of about 48 months in starting clozapine [35], which could worsen long-term prognosis [36]. Taken together, there is a critical need to develop a medication at least as effective as clozapine but with fewer side effects. In fact, a medication achieving this aim would likely be the first-line antipsychotic therapy.
Clozapine as a Model for Drug Development: Neuroreceptors
Hypotheses on the Superior Efficacy of Clozapine
In order to develop a medication as effective as clozapine with fewer side effects, we need to understand clozapine’s mechanism of action. Importantly, to date, no antipsychotic medication approved for the treatment of schizophrenia has been efficacious unless it binds to the dopamine D2 receptor [37]. However, the strength and pattern of binding with other neuroreceptors influences the efficacy and side-effect profile (Table 1).
Several hypotheses exist for clozapine’s superior efficacy (Table 2). Studies on antipsychotic pharmacology originally suggested that D2 receptor occupancy of at least 70% was necessary for efficacy [45]. Interestingly, clozapine has < 70% occupancy at the D2 receptor [46, 47], leading to the hypothesis that low D2 receptor occupancy may underlie the superiority of clozapine [48]. However, quetiapine also has a low D2 receptor occupancy but lacks clozapine’s efficacy.
A second hypothesis relates to the rapidity of dissociation from the D2 receptor [49]. Antipsychotics have different dissociation constants, or rates at which they dissociate from receptors. Clozapine has a high dissociation constant for D2, which is even higher than that of dopamine itself [50]. However, this alone cannot explain clozapine’s superior efficacy, as quetiapine also has a comparable dissociation constant.
A third hypothesis suggests that a higher ratio of affinity for the serotonin 5-hydroxytryptamine (5-HT)2A receptor compared with the dopamine D2 receptor mediates clozapine’s superior efficacy [48, 51]. However, most atypical antipsychotics share a high ratio of 5-HT2A/D2 receptor affinity. Moreover, amisulpride, the second most efficacious medication in the ranking meta-analysis by Leucht et al. [23], has little-to-no serotonin receptor affinity. In addition, 5-HT2A receptor antagonists have not been successful in treating psychosis [52].
A fourth hypothesis is centered on the finding that clozapine has high dopamine D4 receptor affinity and has a higher ratio of affinity for the D4 receptor than the D2 receptor [53]. However, olanzapine and ziprasidone also have high affinity for the D4 receptor, and asenapine has a similar ratio of D4/D2 affinity. Also, D4 antagonists have not shown efficacy in clinical trials [54].
Binding at Other Receptors
In addition to dopamine and serotonin receptors, clozapine binds α-adrenergic, muscarinic, and histamine receptors (Table 1). Some evidence suggests that α1 receptors may affect positive symptoms, while α2 receptors may affect negative and cognitive symptoms [55]. However, the α1 antagonist prazosin [56] and α2 agonist guanfacine did not show clear clinical benefit in improving symptoms of schizophrenia [57].
Clozapine and its metabolite N-desmethylclozapine (NDMC) are potent M1 agonists. Rajji et al. [58] found that the ratio of clozapine to NDMC is negatively correlated with working memory performance. Muscarinic agonists have been investigated for their beneficial effects on cognition and psychosis, and the M1/M4 preferring agonist xanomeline led to improvement in cognitive performance, total PANSS scores, and total Brief Psychiatric Rating Scale scores, but also caused significant gastrointestinal intolerance [59, 60]. It is unclear whether affinity of the M1 receptor contributes significantly to clozapine’s unique mechanism of action, as olanzapine shares its high muscarinic affinity without its superior efficacy.
Both clozapine and NDMC are inverse agonists of the H1 and H2 histamine receptors [61]. H2 antagonism showed some clinical benefit in a randomized clinical trial of patients with treatment-resistant schizophrenia [62]. However, olanzapine also has high histamine receptor affinities [63], but without superior efficacy.
It is possible that clozapine’s superior efficacy is mediated by its affinity for a broad array of neuroreceptors. The combination of these receptor affinities may enhance its efficacy, each with additive but variable contributions. However, many of clozapine’s side effects also result from binding to these receptors. Finally, it should be noted that olanzapine has a similar structure to clozapine but is not as efficacious.
Pimavanserin
Pimavanserin is an example of a recently developed medication based on neuroreceptor binding. In the treatment of Parkinson’s disease psychosis (PDP), clozapine and quetiapine are typically used at low doses that only partially block the D2 receptor. However, at these doses, they still have significant serotonin 5-HT2A receptor blockade, and can ameliorate psychosis. This observation led to the hypothesis that a serotonin receptor inverse agonist, which binds to the serotonin receptor and decreases its intrinsic activity, may be useful in treating psychosis. Pimavanserin is a novel serotonin receptor inverse agonist that lacks activity at dopamine, muscarinic, adrenergic, and histamine receptors [64], and has recently been approved for PDP, as it reduced hallucinations and delusions in patients with PDP [65].
While pimavanserin is currently only approved for PDP, evidence suggests that it is effective as an adjunct to other antipsychotics for the treatment of schizophrenia. In a multicenter, randomized double-blind trial with 423 patients, Meltzer et al. [66] determined that the addition of pimavanserin to a dose of risperidone unlikely to treat psychosis [risperidone 2 mg + pimavanserin (RIS2PIM)] was as effective as a dose of risperidone known to be effective for psychosis [risperidone 6 mg + placebo (RIS6PBO)] at the end of 6 weeks. Interestingly, the RIS2PIM group showed a larger proportion of patients with ≥ 20% improvement in the PANSS at day 15 than the RIS6PBO group. Furthermore, the RIS2PIM group had less weight gain and less increase in their prolactin levels than in the RIS6PBO group. These results suggest that pimavanserin is a promising addition to antipsychotic management and strengthen the hypothesis that serotonin receptors contribute to the efficacy of antipsychotics. Although currently no study compares pimavanserin with clozapine, it is unlikely that pimavanserin will show superior efficacy. However, augmenting clozapine with pimavanserin may allow for lower dosing of clozapine and potentially fewer side effects.
There are now over a dozen antipsychotics on the market, with more in the pipeline since the development of clozapine, but none has matched or improved on clozapine’s efficacy. Given this poor track record, it seems unlikely that additional efforts focused on altering neuroreceptor binding profiles will achieve success. This highlights the need to develop new approaches to antipsychotic development based on clozapine.
Clozapine as a Model for Drug Development: Frontline Approaches
The superior efficacy of clozapine can be investigated in 2 ways to advance the treatment of schizophrenia. One is to understand the genetics of patients that show response or side effects on clozapine. This approach would maximize the successful use of clozapine, providing an important framework for the implementation of personalized medicine in psychiatric care. The second is to understand the molecular mechanisms of clozapine, from receptor binding through downstream genes and pathways. This approach could identify new and specific targets for medications that provide improved efficacy and reduced side effects. To better understand the response, side effects, and mechanism of action of clozapine, the following approaches have been utilized: 1) the pharmacogenetic approach—hypothesis-driven candidate gene association studies; 2) the pharmacogenomic approach—hypothesis-free genome-wide association studies (GWAS); and 3) gene expression profiling—unbiased studies such as microarray and RNA-seq.
Pharmacogenetics
Pharmacogenetic approaches related to clozapine have focused on identifying genetic variants that could predict therapeutic response or the likelihood of developing side effects. To date, pharmacogenetic research has emphasized the major neurotransmitter systems involved in the pharmacodynamics and pharmacokinetics of antipsychotics. While issues with consistency exist in the findings [67,68,69,70], many of these associations could still be important. Several reasons may underlie the inconsistent results, such as different duration of clozapine treatment among the studies, different inclusion criteria for clozapine responders [23], and different ethnicities [71,72,73,74].
There are a number of reviews in the literature that discuss pharmacogenetics in more detail [70, 75,76,77]. Here we highlight some of the most recent systematic reviews and meta-analyses [67,68,69]. Potentially the most significant results regarding pharmacogenetics come from the serotonergic system and its genetic variants (rs6313 and rs6314) in the HTR2A gene, which encodes the 5-HT2A receptor. Interestingly, the first meta-analysis published in 1998 [75] and the most recent meta-analysis from 2016 [67] supported results that the GG genotype of rs6313 and T allele carriers of rs6314 have poorer response on clozapine. However, some studies found negative associations [71, 72, 78,79,80,81].
Regarding clozapine pharmacokinetics, a recent systematic review revealed that genetic variants of the CYP450 system did not show reproducible results but identified novel findings for the ABCB1 gene, which encodes a P-glycoprotein drug transporter [69]. Krivoy et al. [69] highlighted that the ABCB1 gene was the only gene involved in pharmacokinetics found to be significant for both clozapine response and clozapine plasma concentrations. However, negative associations in the Japanese [73] and Chinese Han populations [74] were also recently reported.
Pharmacogenetic studies have elucidated some of the genetics of response and side effects to clozapine. However, these studies have focused mainly on the neurotransmitter systems thought to be relevant to clozapine’s therapeutic response, and identification of novel targets is limited by a candidate approach. As antipsychotics that were developed based on fine-tuning the neurotransmitter receptor binding profile have not improved on the efficacy of clozapine, further candidate studies on neurotransmitter systems are unlikely to yield new insight on clozapine’s mechanism of action or side effects. Therefore, new approaches are needed to match or improve on clozapine’s efficacy while reducing its side effects. In the next subsection, we describe how unbiased approaches can lead to a better understanding of drug design and new therapeutic targets.
Pharmacogenomics
Unbiased information about the mechanisms of clozapine’s efficacy and side effects can be obtained through pharmacogenomics and gene expression profiling. These studies have the potential to identify biological properties of clozapine and etiological factors of schizophrenia. They also provide novel targets from neuroreceptors to downstream genes and pathways, which are not limited by a candidate approach. Insight gained from the pharmacogenomics approach could therefore help inform drug development. We now highlight unbiased genomic studies utilizing human patients treated with clozapine or human brain tissue from patients treated with clozapine.
Response
Schizophrenia is likely caused by a combination of genetic and environmental influences [82,83,84]. From a genetic standpoint, schizophrenia is most often caused by many common genetic variants, each with a small effect size [85]. One way to assess the genetic burden of an individual is to use polygenic risk scores (PRS), or the aggregated genetic load of trait-associated alleles across many loci [86, 87]. From a clinical perspective, the greater the PRS, the higher the disease risk. PRS are obtained by selecting single-nucleotide polymorphisms (SNPs) associated with a phenotype of interest from GWAS samples and creating a sum of their phenotype associated alleles [86, 88]. This is important because many SNPs may not meet genome-wide significance alone, but together they may be significant. PRS have been used for a number of studies addressing the genetic bases of mental illness [85, 88].
Recently, Frank et al. [89] revealed increased PRS, using data from a GWAS of schizophrenia, in patients with a history of clozapine treatment versus clozapine-naive patients. In addition, they found increased PRS in clozapine responders versus nonresponders. These data provide potential insights into the biology of refractory schizophrenia, suggesting that patients who require clozapine have a higher genetic risk burden. These results are relevant for drug development as higher PRS may indicate that multiple target genes and pathways are involved in severe cases of schizophrenia, and could require a combination of medications for treatment. This further necessitates understanding the downstream targets of clozapine to design more effective medications.
Furthermore, Ruderfer et al. [90] demonstrated that increased genetic risk variants track with clozapine treatment. They used the significant genomic regions identified from the GWAS by the Psychiatric Genomics Consortium as risk loci, and compared them with gene sets grouped by antipsychotic targets, efficacy, and pharmacokinetic profiles. Interestingly, they observed enrichment of risk loci for antipsychotic targets, revealing genetic overlap between the etiology of schizophrenia and the mechanisms of action of antipsychotics, which is in line with a previous study [91]. Utilizing clozapine as a proxy for identifying cases of treatment refractory schizophrenia, they observed enrichment of rare disruptive variants in gene sets for antipsychotic targets and efficacy in the clozapine group versus the nonclozapine group. Interestingly, similar results were reported by Martin and Mowry [92], who observed increased burden of rare duplications in treatment-resistant schizophrenia. These studies also provide insights into drug development. Demonstrating a genetic overlap between the mechanisms of action of antipsychotic medications and the pathogenesis of schizophrenia supports the idea that understanding clozapine’s mechanism of action could improve the treatment of schizophrenia.
Importantly, significant genetic results have already begun to emerge from GWAS related to clozapine treatment, and specific alleles have been identified that could lead to new targets. A study in a UK clozapine clinic (CLOZUK) [93] revealed an overlap of about 47% in the SNPs previously reported by the Psychiatric Genomics Consortium. In addition, they identified 3 new loci that met genome-wide significance: inter-alpha-trypsin inhibitor heavy chain 3/4 (ITIH3/4); calcium voltage-gated channel subunit alpha1 C (CACNA1C); and serologically defined colon cancer antigen 8 (SDCCAG8). Moreover, a recent study found an association between a previously reported genetic variant in ITIH3/4 and improvement of negative symptoms in patients treated with clozapine [94]. Another study revealed an association between clozapine efficacy and a genetic variant in D2DR, a risk variant for schizophrenia with genome-wide significance [95]. Studies such as these can help provide targets and potential pathways amenable to intervention both at and beyond neuroreceptors.
Side Effects
To better understand the mechanism of clozapine’s efficacy, it is critical to understand the biology of its side effects for several reasons. First, there may be a biological relationship between clozapine’s efficacy and side effects. Evidence exists for a positive correlation between antipsychotic efficacy and metabolic side effects [96, 97]. This relationship is complex, but may be mediated by molecules such as insulin-like growth factor [98], Akt [99, 100], and glycogen synthase kinase, which have all been linked to schizophrenia and glucose metabolism [101,102,103]. Second, dissecting the pathways mediating therapeutic response to clozapine from those mediating its side effects could lead to improved medications, or possibly a combination of medications, that could produce the same response without the side effects. Third, understanding which patients are likely to develop side effects could optimize the clinical use of clozapine, such as developing treatment or prophylaxis protocols for specific individuals depending on their likelihood of developing certain side effects.
To date, the only clozapine-induced side effects studied in an unbiased manner are neutropenia and weight gain. Results from 2 different GWAS investigations demonstrated the association of human leukocyte antigen (HLA) genetic variants with clozapine-induced agranulocytosis or neutropenia [104, 105], with some of the most promising SNPs occurring in the HLA-DQB1 and HLA-B loci. Additionally, a novel SNP intronic to solute carrier organic anion transporter family member 1B3 (SLCO1B3) and solute carrier organic anion transporter family member 1B7 (SLCO1B7), both members of a family of organic anion hepatic transporters, was identified through the GWAS. Furthermore, an exome array analysis found that ubiquitin associated protein 2 and StAR-related lipid transfer domain containing 9 are associated with clozapine-induced neutropenia.
For clozapine-induced weight gain, melanocortin 4 receptor has emerged as a promising gene. It was originally identified from a GWAS of obesity [106] and substantiated in a large GWAS of antipsychotic-induced weight gain [107]. Later, Chowdhury et al. [108] demonstrated the role of melanocortin 4 receptor specifically in clozapine-induced weight gain.
Similar to identifying novel targets for response, identifying specific targets and pathways related to side effects can facilitate the development of novel antipsychotics. So far, while limited in scope, the unbiased studies described above provide insights into the genetics related to clozapine’s side effects and could be critical in developing novel medications as we better understand the genes and pathways related to clozapine’s side effects.
Gene Expression Profiling
In addition to pharmacogenomics, gene expression profiling can help to determine the mechanisms of clozapine’s superior efficacy beyond neuroreceptor binding. Gene expression profiling can identify specific genes and pathways related to clozapine’s mechanism of action, providing novel drug targets. Several studies have examined gene expression changes in postmortem brains of patients on antipsychotic therapy versus healthy controls [109,110,111,112,113,114,115,116]. However, no study of gene expression in the human brain specifically compared patients treated with clozapine with patients treated with other antipsychotics, or with healthy controls.
Therefore, Lee et al. [117] recently performed the first study to analyze gene expression changes specifically in response to clozapine in the human brain. This work extracted primary data from 4 microarray studies of postmortem brains, and analyzed the expression data from patients on clozapine versus patients on other atypical antipsychotics. Each study highlighted a number of genes modulated by clozapine, many of which could be of interest, but 3 results were identified in all 4 of the studies analyzed: glutamate-cysteine ligase modifier subunit (GCLM) and zinc finger protein 652 (ZNF652) were found to be upregulated, and glycophorin C (GYPC) was found to be downregulated. Four pathways were also highlighted in all of the studies: clathrin-mediated endocytosis, stress-activated protein kinase/c-Jun-terminal kinase signaling, 3-phosphoinositide biosynthesis, and paxillin signaling. Interestingly many of these genes and pathways have been implicated in schizophrenia, further validating this approach [118,119,120,121,122,123].
Studies as described above provide targets and pathways related to clozapine’s impact on gene expression, which are potentially amenable to therapeutic intervention. Developing novel medications that change the regulation of genes altered in schizophrenia, or are affected by clozapine, could yield superior therapies with targets beyond neuroreceptor systems.
Summary of Frontline Approaches
Both pharmacogenetics and pharmacogenomics have strengths in understanding clozapine’s mechanism of action, providing strategies to identify therapeutic targets and develop new medications. Pharmacogenetics has provided insights into the genetics of response and side effects, mainly with candidate genes related to neuroreceptors.
Pharmacogenomics and gene expression profiling provide useful tools to identify novel targets for therapeutic intervention in an unbiased manner. The studies described above highlight several key points. First, there is genetic overlap between the mechanisms of action of antipsychotics and the pathogenesis of schizophrenia. This supports the idea that understanding clozapine’s mechanism of action could not only lead to valid therapeutic targets, but also elucidate the complex pathophysiology of schizophrenia. Second, higher rates of PRS are associated with refractory schizophrenia and clozapine use, indicating that medications with multiple targets or a combination of medications may be necessary to treat severe cases of schizophrenia. It also suggests that higher PRS could serve as a useful marker for prescribing clozapine as a first-line antipsychotic. Third, pathways other than neurotransmitter systems are important, as most of the genes or pathways described above are not directly linked to the neurotransmitter receptors thought to underlie clozapine’s superior efficacy. Finally, novel genes and pathways have begun to emerge from these unbiased studies. All of these studies provide evidence that further investigation into the functional implication of these genetic variants, and identifying specific genes and pathways from gene expression profiling, would help elucidate clozapine’s mechanism of action, leading to novel therapeutic targets and potentially new and more efficacious medications.
Future Studies and Novel Approaches
Unfortunately, decades of altering neuroreceptor profiles have not led to medications with greater efficacy than clozapine. The studies described in this review begin to offer clues to clozapine’s mechanism of action. Further studies using unbiased approaches to identify genes and pathways, or addressing promising candidate genes beyond neuroreceptors, are needed to obtain a fuller understanding of clozapine’s mechanism of action and to identify drug targets. Utilizing biospecimens, future studies could introduce patient stratification based on clinical symptomatology, response to clozapine, or side effects. Stratifying patients in these ways would provide a fuller picture of clozapine’s mechanism of action and delineate, for example, response from side effects. Pre- and post-clozapine experimental design would eliminate interindividual differences, and longitudinally capture genes and pathways contributing to response, side effects, or a common mechanism mediating both. Once patients are stratified, pharmacogenetics, pharmacogenomics, or gene expression profiling can be performed. Information identified from molecular and preclinical studies, which are beyond the scope of this review, could also help inform our understanding of clozapine and aid the development of potential candidate targets. The most promising targets from all of these approaches can be further studied in model systems and then utilized for drug development.
In the 30 years since the first landmark study on clozapine in the US, over a dozen antipsychotics have been marketed, without succeeding in improving on clozapine’s efficacy. Therefore, approaches that can identify specific genes and pathways, as novel drug targets beyond neuroreceptor binding, hold promise to elucidate the mechanisms of clozapine’s action and side effects, as well as the complex pathophysiology of schizophrenia. Insights gained from these approaches are critical for the development of novel therapeutics that could advance the current state of antipsychotic therapy.
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Acknowledgments
This work was supported by the National Institute of Mental Health MH-094268 Silvio O. Conte center, MH-092443, MH-105660 (AS); National Institute on Drug Abuse DA-040127 (AS); foundation grants from Stanley, S-R, RUSK, NARSAD, Maryland Stem Cell Research Fund (AS). We would also like to thank Yukiko Lema for help with this manuscript.
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Nucifora, F.C., Mihaljevic, M., Lee, B.J. et al. Clozapine as a Model for Antipsychotic Development. Neurotherapeutics 14, 750–761 (2017). https://doi.org/10.1007/s13311-017-0552-9
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DOI: https://doi.org/10.1007/s13311-017-0552-9