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Tumor Biology

, Volume 37, Issue 12, pp 15977–15987 | Cite as

Long noncoding RNA expression profile of infantile hemangioma identified by microarray analysis

  • Xiaowen Liu
  • Renrong Lv
  • Linfeng Zhang
  • Guangqi Xu
  • Jianhai Bi
  • Feng Gao
  • Jian Zhang
  • Feng Xue
  • Fagang Wang
  • Yiliang Wu
  • Cong Fu
  • Qiang Wang
  • Ran Huo
Original Article

Abstract

Infantile hemangioma (IH) is one of the most common vascular tumors of childhood. Long noncoding RNAs (lncRNAs) play a critical role in angiogenesis, but their involvement in hemangioma remains unknown. This study aimed to assess the expression profiles of lncRNAs in IH and adjacent normal tissue samples, exploring the biological functions of lncRNAs as well as their involvement in IH pathogenesis. The lncRNA expression profiles were determined by lncRNA microarrays. A total of 1259 and 857 lncRNAs were upregulated and downregulated in IH, respectively, at a fold change cutoff of 2.0 (p < 0.05); in addition, 1469 and 1184 messenger RNAs (mRNAs) were upregulated and downregulated, respectively (fold change cutoff of 2.0; p < 0.05). A total of 292 differentially expressed mRNAs were targeted by the lncRNAs with altered expression in hemangioma, including 228 and 64 upregulated and downregulated, respectively (cutoff of 2.0, p < 0.05). Gene ontology (GO) analyses revealed several angiogenesis-related pathways. An lncRNA-mRNA co-expression network for differentially expressed lncRNAs revealed significant associations of the lncRNAs MEG3, MEG8, FENDRR, and Linc00152 with their related mRNAs. The validation results of nine differentially expressed lncRNAs (MALAT1, MEG3, MEG8, p29066, p33867, FENDRR, Linc00152, p44557_v4, p8683) as well as two mRNAs (FOXF1, EGFL7) indicated that the microarray data correlated well with the QPCR results. Interestingly, MALAT1 knockdown induced apoptosis and S-phase cell cycle arrest in human umbilical vein endothelial cells (HUVECs). Overall, this study revealed the lncRNA expression profile of IH and that lncRNAs likely regulate several genes with important roles in angiogenesis.

Keywords

Long noncoding RNA MALAT1 Infantile hemangioma Angiogenesis Microarrays 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 81171828).

Compliance with ethical standards

Funding

This work was supported by the National Natural Science Foundation of China (Grant No. 81171828).

Conflicts of interest

None

Ethical approval

This study was approved by the ethics committee of Shandong Provincial Hospital, China (No. 2014-011).

Informed consent

Patient guardians provided verbal and written informed consent.

Supplementary material

13277_2016_5434_MOESM1_ESM.docx (18 kb)
ESM 1 (DOCX 18 kb)
13277_2016_5434_Fig6_ESM.gif (352 kb)
Supplementary Figure 1

Hierarchical clustering analysis of 2116 differentially expressed long noncoding RNAs (lncRNAs) and 2653 differentially expressed mRNAs. Red and green indicate increased and reduced expression levels, respectively. In the heat map, columns represent samples and rows are the various genes. Scale of expression level is shown on the horizontal bar. (GIF 352 kb)

13277_2016_5434_MOESM2_ESM.tif (5 mb)
High resolution image (TIFF 5128 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Xiaowen Liu
    • 1
  • Renrong Lv
    • 1
  • Linfeng Zhang
    • 1
  • Guangqi Xu
    • 1
  • Jianhai Bi
    • 1
  • Feng Gao
    • 1
  • Jian Zhang
    • 1
  • Feng Xue
    • 1
  • Fagang Wang
    • 1
  • Yiliang Wu
    • 1
  • Cong Fu
    • 1
  • Qiang Wang
    • 2
  • Ran Huo
    • 1
  1. 1.Department of Burn & Plastic SurgeryShandong Provincial Hospital Affiliated to Shandong UniversityJinanChina
  2. 2.Department of Burn and Plastic SurgeryTengzhou Central People’s HospitalTengzhouChina

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