Tumor Biology

, Volume 37, Issue 12, pp 16337–16343 | Cite as

FAT4 functions as a tumor suppressor in triple-negative breast cancer

Original Article

Abstract

Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer that is often associated with biologic behavior with frequent distant metastasis. FAT tumor suppressor homolog 4 (FAT4), a cadherin-related protein, is involved in a variety of biological processes as a tumor suppressor; however, the role of FAT4 in TNBC is still unclear. The aim of our study was to identify the role of FAT4 in TNBC and examine the underlying molecular mechanisms. The expression of FAT4 was evaluated by immunohistochemistry, western blotting, and qRT-PCR in a series of TNBC tissues. The effects of FAT4 on the ability of cell proliferation, migration, and invasion were assessed by MTT assay and migration and invasion assays. We demonstrated that the repression of FAT4 by shRNA could promote TNBC progression. Taken together, our findings provide evidence for a role of the FAT4 cluster as a tumor suppressor in TNBC patients and may serve as potential novel targets for the treatment of TNBC.

Keywords

TNBC FAT4 Invasion Proliferation 

Notes

Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (Grants No. 81172496), grants from Education Department of Sichuan Province (Grants No. 13ZA0228) and “Innovation talent Project” from Technological Office of Sichuan Province (Grants No. 2016060).

Compliance with ethical standards

All samples were collected with patients’ informed consent, and this project was approved by the Ethics Committee of The Third Military Medical University.

Conflicts of interest

None.

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Lingmi Hou
    • 1
    • 2
    • 3
  • Maoshan Chen
    • 4
  • Xiaobo Zhao
    • 2
  • Jingdong Li
    • 3
  • Shishan Deng
    • 5
  • Jiani Hu
    • 6
  • Hongwei Yang
    • 4
  • Jun Jiang
    • 1
  1. 1.Breast Disease Center, Southwest HospitalThird Military Medical UniversityChongqingChina
  2. 2.Department of Thyroid Breast SurgeryAffiliated Hospital of North Sichuan Medical CollegeNanchongChina
  3. 3.Institute of Hepatobiliary Pancreatic-Intestinal DiseasesAffiliated Hospital of North Sichuan Medical CollegeNanchongChina
  4. 4.Department of Breast and Thyroid SurgerySuining Central HospitalSuiningChina
  5. 5.Department of AnatomyThe North Sichuan Medical CollegeNanchongChina
  6. 6.Department of RadiologyWayne State UniversityDetroitUSA

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