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Association of single-nucleotide polymorphisms of high-mobility group box 1 with susceptibility and clinicopathological characteristics of uterine cervical neoplasia in Taiwanese women

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Tumor Biology

Abstract

To date, no study associated the genetic polymorphisms of high-mobility group box 1 protein (HMGB1) with the development of uterine cervical cancer. We therefore conducted this study to investigate the associations of HMGB1 single-nucleotide polymorphisms (SNPs) with cervical carcinogenesis and clinicopathological characteristics of cancer patients. Five hundred two women, including 112 with invasive cancer, 85 with precancerous lesions of the uterine cervix, and 305 normal controls, were consecutively enrolled into this study. Analysis of HMGB1 SNPs was done by real-time polymerase chain reaction and genotyping. Our results found that the risk of susceptibility to cervical invasive cancer was 1.85 (95 % CI 1.12–3.04; p = 0.016) in women with TC and 1.99 (95 % CI 1.24–3.23; p = 0.005) in women with TC/CC after adjusting for age, using TT as a comparison reference in HMGB1 SNP rs1412125. In rs2249825, the increased risk was also seen for the development of cervical invasive cancer in women with CG [adjusted odds ratio (AOR) 2.04, 95 % CI 1.22–3.40; p = 0.006] or CG/GG (AOR 2.02, 95 % CI 1.22–3.32; p = 0.006) using CC as a comparison reference. An additional integrated in silico analysis confirmed that rs2249825 creates a binding site for v-Myb, which may affect HMGB1 expression. In conclusion, Taiwanese women with TC or TC/CC in HMGB1 SNP rs1412125 as well as CG or CG/GG in rs2249825 were susceptible to the development of cervical invasive cancer.

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Authors and Affiliations

  1. Institute of Medicine, Chung Shan Medical University, 110, Section 1, Chien-Kuo North Road, Taichung, 40201, Taiwan

    Hsin-Hung Wu, Shun-Fa Yang, Shiuan-Chih Chen, Hsiang-Ling Wang, Long-Yau Lin & Po-Hui Wang

  2. Infertility Center, Xiamen EMBO Hospital Xiamen, Fujian, China

    Hsin-Hung Wu

  3. Reproductive Medicine Center, Kinmen Hospital, Kinmen, Taiwan

    Hsin-Hung Wu

  4. Department of Biomedical Sciences, Chung Shan Medical University, Taichung, Taiwan

    Yu-Fan Liu

  5. Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan

    Yu-Fan Liu & Shun-Fa Yang

  6. School of Medicine, Chung Shan Medical University, Taichung, Taiwan

    Wea-Lung Lin, Shiuan-Chih Chen, Chih-Ping Han, Long-Yau Lin & Po-Hui Wang

  7. Department of Pathology, Chung Shan Medical University Hospital, Taichung, Taiwan

    Wea-Lung Lin & Chih-Ping Han

  8. Department of Family and Community Medicine, Chung Shan Medical University Hospital, Taichung, Taiwan

    Shiuan-Chih Chen

  9. Department of Obstetrics and Gynecology, Chung Shan Medical University Hospital, Taichung, Taiwan

    Chih-Ping Han, Long-Yau Lin & Po-Hui Wang

  10. Department of Beauty Science, National Taichung University of Science and Technology, Taichung, Taiwan

    Hsiang-Ling Wang

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  1. Hsin-Hung Wu
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Correspondence to Long-Yau Lin or Po-Hui Wang.

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The Chung Shan Medical University Hospital Institutional Review Board approved this study, and informed written consents were obtained from all subjects (CSMUH IRB: CS12219, CS14014)

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Wu, HH., Liu, YF., Yang, SF. et al. Association of single-nucleotide polymorphisms of high-mobility group box 1 with susceptibility and clinicopathological characteristics of uterine cervical neoplasia in Taiwanese women. Tumor Biol. 37, 15813–15823 (2016). https://doi.org/10.1007/s13277-016-5408-0

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  • DOI: https://doi.org/10.1007/s13277-016-5408-0

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