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RETRACTED ARTICLE: MiR-429 induces apoptosis of glioblastoma cell through Bcl-2

  • Original Article
  • Published:
Tumor Biology

This article was retracted on 20 April 2017

Abstract

An essential role of microRNAs (miRNAs) has been acknowledged in the tumorigenesis of glioblastoma multiforme (GBM). Very recently, miR-429 was reported to have a potential of suppressing cancer growth. However, whether miR-429 may similarly regulate growth of GBM remains unknown. Here, we analyzed the levels of miR-429 and anti-apoptotic protein Bcl-2 in GBM specimens. We combined bioinformatics analyses and luciferase reporter assay to determine the relationship between miR-429 and Bcl-2 in GBM cells. Cell survival upon temozolomide treatment was analyzed in a CCK assay. Cell apoptosis was measured by fluorescein isothiocyanate (FITC) Annexin V apoptosis detection assay. We found that miR-429 levels were significantly decreased and Bcl-2 levels were significantly increased in GBM specimens, compared to the paired adjacent non-tumor brain tissue. Moreover, the levels of miR-429 and Bcl-2 inversely correlated. Low-miR-429 subjects had an overall inferior survival, compared to high-miR-429 subjects. MiR-429 targeted the 3′-UTR of Bcl-2 mRNA to inhibit its translation. Overexpression of miR-429 inhibited Bcl-2-mediated cell survival against temozolomide-induced apoptosis, while depletion of miR-429 augmented it. Together, our data suggest that miR-429 suppression in GBM promotes Bcl-2-mediated cancer cell survival against chemotherapy-induced cell death. Re-expression of miR-429 levels in GBM cells may improve the outcome of chemotherapy.

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Acknowledgments

This work has been supported by Program for Excellent Talents in Liaoning Province in China (No. LJQ2013088).

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Authors and Affiliations

  1. Department of Neurosurgery, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, 121001, China

    Zhenxing Zhang, Qingqing Zhou, Ye Miao, Yang Li & Xu Feng

  2. Department of Histology and Embryology, Liaoning Medical University, 3-40 Songpo Road, Jinzhou, 121001, China

    He Tian & Xiaofeng Song

Authors
  1. Zhenxing Zhang
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  2. Qingqing Zhou
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  3. Ye Miao
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  4. He Tian
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  5. Yang Li
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  7. Xiaofeng Song
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Correspondence to Xiaofeng Song.

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Additional information

The Publisher and Editor retract this article in accordance with the recommendations of the Committee on Publication Ethics (COPE). After a thorough investigation we have strong reason to believe that the peer review process was compromised.

An erratum to this article is available at http://dx.doi.org/10.1007/s13277-017-5487-6.

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Zhang, Z., Zhou, Q., Miao, Y. et al. RETRACTED ARTICLE: MiR-429 induces apoptosis of glioblastoma cell through Bcl-2. Tumor Biol. 37, 15607–15613 (2016). https://doi.org/10.1007/s13277-015-4291-4

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  • DOI: https://doi.org/10.1007/s13277-015-4291-4

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