Tumor Biology

, Volume 37, Issue 12, pp 15583–15591 | Cite as

MYSM-1 suppresses migration and invasion in renal carcinoma through inhibiting epithelial-mesenchymal transition

  • Lei Zhou
  • Liyin Shi
  • Hua Guo
  • Xin Yao
Original Article


Renal cell carcinoma (RCC) is the most common malignant renal tumor and is prone to metastasis. However, the molecular variation and mechanism underlying renal cell carcinoma metastasis remains largely unknown. In our previous study, it was found that MYSM-1 was significantly downregulated in renal cell carcinoma tissues as compared with normal renal tissues without metastasis, using proteomics approach. Therefore, we hypothesized that MYSM-1 may suppress the metastasis of renal cell carcinoma in light of paucity of data regarding MYSM-1 in the cancers. In the present study, to confirm the expression status of MYSM-1 in renal cell carcinoma, immunohistochemistry with renal carcinoma tissue microarray was performed. It was shown that MYSM-1 was remarkably decreased in renal carcinoma tissues compared with paired normal control tissues; and that low expression of MYSM-1 was significantly associated with poor overall prognosis and metastasis. To investigate the biological roles of MYSM-1 in vitro in renal carcinoma cell lines, both knockdown using siRNA and over-expression were carried out. It was found that MYSM-1 could suppress the proliferation, migration, and invasion of renal carcinoma cells. In addition, we found that MYSM-1 could inhibit the epithelial-mesenchymal transition. Together, our results demonstrate that MYSM-1 could suppress the metastasis of renal carcinoma cells may be through inhibiting the epithelial-mesenchymal transition (EMT) process.


Renal carcinoma MYSM-1 Metastasis Prognosis EMT 



The work was supported by National Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and Therapy project.

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

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Supplementary Table 1 (DOCX 13kb)
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Supplementary Table 2 (DOCX 13kb)
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Supplementary Table 3 (DOCX 14kb)
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Supplementary Fig. 4 Evaluation of the specificity of primary antibody againt MYSM-1 that was used in the present study before immunohistochemistry. (DOCX 704kb)


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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  1. 1.National Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and TherapyTianjin Medical University Cancer Institute and HospitalTianjinChina
  2. 2.The Department of Microbiology, College of Basic MedicineTianjin Medical UniversityTianjinChina

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