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Tumor Biology

, Volume 37, Issue 12, pp 15567–15573 | Cite as

Pituitary tumor-transforming gene 1 enhances metastases of cervical cancer cells through miR-3666-regulated ZEB1

  • Lin Li
  • Li-Ying Han
  • Ming Yu
  • Qi Zhou
  • Jian-Cheng Xu
  • Ping Li
Original Article

Abstract

Early cancer metastases often occur in cervical cancer (CC) patients, resulting in poor prognosis and poor therapeutic outcome after resection of primary cancer. Hence, there is a compelling requirement for elucidating the molecular mechanisms underlying the CC cell invasiveness. Recently, the role of microRNAs (miRNAs) and pituitary tumor-transforming gene 1 (Pttg1) in the carcinogenesis of CC has been reported. Nevertheless, the relationship between miRNAs and Pttg1 remains ill-defined. Here, we showed that the levels of miR-3666 were significantly decreased and the levels of zinc finger E-box binding homeobox 1 (ZEB1) and Pttg1 were significantly increased in the CC specimens from patients, compared to the paired non-tumor tissue. Moreover, the levels of miR-3666 and ZEB1 inversely correlated. Bioinformatics analyses showed that miR-3666 targeted the 3′-untranslated region (3′-UTR) of ZEB1 messenger RNA (mRNA) to inhibit its translation, which was confirmed by luciferase reporter assay. Moreover, Pttg1 overexpression inhibited miR-3666 and subsequently increased ZEB1 and cell invasion, while Pttg1 depletion increased miR-3666 and subsequently decreased ZEB1 and cell invasion. Together, our data suggest that Pttg1 may increase CC cell metastasis, possibly through miR-3666-regulated ZEB1 levels.

Keywords

Cervical cancer (CC) ZEB1 miR-3666 Pttg1 

Notes

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Lin Li
    • 1
  • Li-Ying Han
    • 2
  • Ming Yu
    • 3
  • Qi Zhou
    • 4
  • Jian-Cheng Xu
    • 1
  • Ping Li
    • 5
  1. 1.Department of Medical Laboratorythe First Hospital of Jilin UniversityChangchunChina
  2. 2.Department of Obstetrics and Gynecologythe Second Hospital of Jilin UniversityChangchunChina
  3. 3.The Affiliated Hospital to Changchun University of Chinese MedicineChangchunChina
  4. 4.Department of Pediatricsthe First Hospital of Jilin UniversityChangchunChina
  5. 5.Doctrine Department, Chinese Medical SchoolsChangchun University of Chinese MedicineChangchunChina

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