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Tumor Biology

, Volume 37, Issue 12, pp 15509–15515 | Cite as

MiR-153 regulates metastases of gastric cancer through Snail

  • Zhongli Wang
  • Chao Liu
Original Article

Abstract

MicroRNAs (miRNAs) play critical roles in tumorigenesis and cancer metastases of gastric carcinoma (GC). Recently, miR-153 was reported as a tumor suppressor miRNA silenced in GC, whereas the underlying mechanisms are much lacking. Here, we reported that miR-153 levels were significantly decreased and Snail levels were significantly increased in GC specimens, compared to paired adjacent non-tumor gastric tissue. Moreover, the levels of miR-153 and Snail were inversely correlated. Bioinformatics analyses and luciferase reporter assay showed that miR-153 targeted the 3′-untranslated region (3′-UTR) of Snail messenger RNA (mRNA) to inhibit its translation. Overexpression of miR-153 inhibited Snail-mediated cell invasiveness, while depletion of miR-153 increased Snail-mediated cell invasiveness in both Transwell cell migration assay and scratch wound healing assay. Thus, our data suggest that miR-153 suppression in GC promotes Snail-mediated cancer metastases. Re-establishment of miR-153 levels in GC may suppress cancer invasion.

Keywords

Gastric carcinoma (GC) Snail MiR-153 

Notes

Conflicts of interest

None

Compliance with ethical standards

Patient’s consents and approval from the Institutional Research Ethics Committee were obtained.

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  1. 1.Department of General Surgerythe First Affiliated Hospital of Liaoning Medical UniversityJinzhouChina
  2. 2.Department of Developmental BiologyLiaoning Medical UniversityJinzhouChina

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