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RETRACTED ARTICLE: Impairment of growth of gastric carcinoma by miR-133-mediated Her-2 inhibition

  • Research Article
  • Published:
Tumor Biology

This article was retracted on 20 April 2017

Abstract

Gastric carcinoma (GC) is a leading cause of cancer-related death in China. Dysregulation of microRNAs (miRNAs) has been shown to contribute to the development of GC, whereas the role of miR-133 in GC is unknown. Here, we analyzed the levels of miR-133 in GC tissues by reverse and quantitative transcription polymerase chain reaction (RT-qPCR). We overexpressed or inhibited miR-133 in GC cells. Cell growth was analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and cell apoptosis was evaluated by fluorescence-activated cell sorting (FACS) analysis. Targeted genes were predicted by a bioinformatics algorithm and confirmed by a dual-luciferase reporter assay. We detected lower miR-133 levels in GC tissues compared with normal gastric tissue. Moreover, the low miR-133 levels were correlated with low survival rate. Overexpression of miR-133 inhibited cell growth and promoted apoptosis, while depletion of miR-133 increased cell growth and suppressed apoptosis. Moreover, the 3′-untranslated region (3′UTR) of Her-2, the epidermal growth factor receptor (EGFR) that transduces cell growth signals, appeared to be targeted by miR-133. Together, these data suggest that reduced miR-133 levels in GC tissues promote GC growth, which possibly contributes to a low survival rate of GC patients. MiR-133 may target Her-2 to suppress GC cell growth.

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Authors and Affiliations

  1. College of Medicine and Pharmaceutics, Ocean University of China, Qingdao, 266003, China

    Xiao-Tao Zhang, Yong-Ning Xin & Shi-Ying Xuan

  2. Qingdao Cancer Hospital, Qingdao, 266042, China

    Xiao-Tao Zhang, Zhen Zhang & Xue-Zhen Ma

  3. Qingdao Municipal Hospital, Qingdao, 266021, China

    Yong-Ning Xin & Shi-Ying Xuan

Authors
  1. Xiao-Tao Zhang
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  2. Zhen Zhang
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  3. Yong-Ning Xin
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  4. Xue-Zhen Ma
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  5. Shi-Ying Xuan
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Corresponding author

Correspondence to Shi-Ying Xuan.

Additional information

The Publisher and Editor retract this article in accordance with the recommendations of the Committee on Publication Ethics (COPE). After a thorough investigation we have strong reason to believe that the peer review process was compromised.

An erratum to this article is available at http://dx.doi.org/10.1007/s13277-017-5487-6.

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Zhang, XT., Zhang, Z., Xin, YN. et al. RETRACTED ARTICLE: Impairment of growth of gastric carcinoma by miR-133-mediated Her-2 inhibition. Tumor Biol. 36, 8925–8930 (2015). https://doi.org/10.1007/s13277-015-3637-2

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  • DOI: https://doi.org/10.1007/s13277-015-3637-2

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