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RETRACTED ARTICLE: Regulation of MET-mediated proliferation of thyroid carcinoma cells by miR-449b

  • Research Article
  • Published:
Tumor Biology

This article was retracted on 20 April 2017

Abstract

Thyroid carcinoma (TC) is a lethal cancer worldwide, whereas its carcinogenesis is not fully understood. Although growing evidence has demonstrated that dysregulation of microRNAs (miRNAs) contributes to the development of various cancers, the role of miRNAs in the pathogenesis of TC is poorly characterized. Here, we analyzed the levels of miR-449b in TC tissues and detected significantly lower miR-449b levels in TC tissues. Moreover, the low miR-449b levels were associated with poor survival. We then overexpressed miR-449b by miRNA mimic transfection and inhibited miR-449b by miRNA antisense transfection. Cell growth was analyzed by CCK-8 assay and MTT assay, and apoptosis and cell proliferation were analyzed by flow cytometry. Overexpression of miR-449b significantly inhibited cell growth, while depletion of miR-449b increased cell growth. Moreover, the effects of miR-449b on cell growth were through modulation of cell proliferation rather than through modulation of cell apoptosis. Targeted genes were predicted by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay, showing that miR-449b binds to the 3′-UTR of MET (hepatocyte growth factor receptor) mRNA, to inhibit its expression in TC cells. MET levels were regulated by miR-449b in TT cells. Together, we show that reduced miR-449b levels in TT tissues may promote TC growth, through MET-mediated cell proliferation.

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Authors and Affiliations

  1. Department of General Surgery, The Tenth People’s Hospital, Tongji University, 301 Middle Yanchang Road, Shanghai, 200072, China

    Lei Chen, Lei Xu & Gang Wang

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  1. Lei Chen
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  2. Lei Xu
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  3. Gang Wang
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Correspondence to Gang Wang.

Additional information

The Publisher and Editor retract this article in accordance with the recommendations of the Committee on Publication Ethics (COPE). After a thorough investigation we have strong reason to believe that the peer review process was compromised.

An erratum to this article is available at http://dx.doi.org/10.1007/s13277-017-5487-6.

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Chen, L., Xu, L. & Wang, G. RETRACTED ARTICLE: Regulation of MET-mediated proliferation of thyroid carcinoma cells by miR-449b. Tumor Biol. 36, 8653–8660 (2015). https://doi.org/10.1007/s13277-015-3619-4

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  • DOI: https://doi.org/10.1007/s13277-015-3619-4

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