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RETRACTED ARTICLE: MicroRNA-92 promotes gastric cancer cell proliferation and invasion through targeting FXR

  • Research Article
  • Published:
Tumor Biology

This article was retracted on 20 April 2017

Abstract

MicroRNAs (miRNAs), a class of small noncoding RNAs, play critical roles in human carcinogenesis through downregulation of various target genes. In the present study, we found that miR-92 is upregulated in gastric cancer tissues compared with adjacent normal tissues. Interestingly, miR-92 expression is significantly associated with clinical characteristics of patients. Gain or loss-of-function in vitro experiments further show that miR-92 mimics significantly promoted, while its antisense oligos inhibited gastric cancer cell proliferation and invasion. Moreover, luciferase reporter assays and western blot indicated that farnesoid X receptor (FXR), is a direct target of miR-92. Therefore, our data suggest that upregulation of miR-92 may represent an important mechanism for the development of gastric cancer.

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Conflict of interest

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Authors and Affiliations

  1. Department of Gastroenterology, Second Hospital of Shaoxing City, Shaoxing, Zhejiang Province, 312000, China

    Jian-Hua Duan

  2. Department of Gastroenterology, China–Japan Friendship Hospital, Beijing, 100029, China

    Long Fang

Authors
  1. Jian-Hua Duan
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  2. Long Fang
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Corresponding author

Correspondence to Jian-Hua Duan.

Additional information

The Publisher and Editor retract this article in accordance with the recommendations of the Committee on Publication Ethics (COPE). After a thorough investigation we have strong reason to believe that the peer review process was compromised.

An erratum to this article can be found online at http://dx.doi.org/10.1007/s13277-017-5487-6.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary Figure 1

a Expression levels of miR-92 after transfection of miR-92 mimics or negative controls (NC) in NCI-N87 cells for 24 h. b, c The proliferative potential (BrdU) (b) and invasion assays (c) were determined in NCI-N87 cells transfected with miR-92 mimics or negative controls (NC) for 24 h. (d) The cell cycle phase of NCI-N87 cells transfected with miR-92 mimics or negative controls (NC) were analyzed by flow cytometry. (GIF 4 kb)

High resolution image (TIFF 420 kb)

Supplementary Figure 2

Representative FXR protein levels in human gastric cancer tissues and adjacent normal tissues from three patients. (GIF 2 kb)

High resolution image (TIFF 198 kb)

Supplementary Figure 3

a, b Relative mRNA (a) and protein (b) levels of C-myc and cyclin D1 were determined by real-time PCR and western blot in AGS cells after transfection of miR-92 mimics or negative controls (NC). (GIF 3 kb)

High resolution image (TIFF 237 kb)

Supplementary Figure 4

a, b Relative mRNA (a) and protein (b) levels of C-myc and cyclin D1 were determined by real-time PCR and western blot in AGS cells after transfection of miR-92 antisense or negative controls (NC). (GIF 2 kb)

High resolution image (TIFF 220 kb)

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Duan, JH., Fang, L. RETRACTED ARTICLE: MicroRNA-92 promotes gastric cancer cell proliferation and invasion through targeting FXR. Tumor Biol. 35, 11013–11019 (2014). https://doi.org/10.1007/s13277-014-2342-x

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  • DOI: https://doi.org/10.1007/s13277-014-2342-x

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