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The immunological and oxidative stress regulation of non-thermal plasma-aided water on atopic dermatitis-like lesion in dinitrochlorobenzene-induced SKH-1 hairless mice

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Abstract

Backgrounds

While non-thermal plasma aided water (NTP-AW) has been currently used as a novel therapeutic against wide array of diseases, it remains unknown on therapeutic effect against atopic dermatitis (AD). To answer this, we evaluated the effect of NTP-AW on the regulation of immune and antioxidant markers in dinitrochlorobenzene (DNCB)-induced AD in hairless mice.

Methods

The skin damage in mice was induced by 1% DNCB for the first wk and 0.5% every alternate day for 3 wks followed by 4 wks NTP-AW treatment through spraying and bathing method.

Results

Treatment with NTP-AW reduced the severity of dermatitis symptoms and skin scratching behavior. In line, immunological profiling showed the improved parameters such as decrease in the number of neutrophils, lymphocyte, eosinophil as well as Th2-mediated IgE overproduction, and the reduced level of pro-inflammatory cytokine such as interleukin (IL)-1β, and tumor necrosis factor (TNF)-α. Redox profiling showed that NTP-AW reduced the production of total reactive oxygen species (ROS), and enhanced the antioxidant enzyme activity.

Conclusion

Collectively, this study clearly indicates the therapeutic effect of NTP-AW on DNCB-induced AD in hairless mice via immuno-redox regulation, clinically implying that NTP-AW treatment might be a safe and promising therapeutic candidate against AD.

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Author notes

  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Environmental Medical Biology, Wonju College of Medicine Yonsei University, Wonju, Gangwon, 26426, Republic of Korea

    Jesmin Ara, Johny Bajgai, Ailyn Fadriquela & Kyu-Jae Lee

  2. Department of Global Medical Science, Wonju College of Medicine Yonsei University, Wonju, Gangwon, 26426, Republic of Korea

    Jesmin Ara, Johny Bajgai & Ailyn Fadriquela

  3. Institute of Biology, University of Philippines, Diliman, Quezon City, 1101, Philippines

    Ma. Easter Joy Sajo

  4. Department of Microbiology, Institute of Genomic Cohort, Wonju College of Medicine Yonsei University, Wonju, Gangwon, 26426, Republic of Korea

    Cheol-Su Kim & Soo-Ki Kim

  5. Institute for Poverty Alleviation and International Development, Yonsei University, Wonju, Gangwon, 26493, Republic of Korea

    Kyu-Jae Lee

Authors
  1. Jesmin Ara
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  2. Johny Bajgai
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Correspondence to Kyu-Jae Lee.

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Ara, J., Bajgai, J., Sajo, M.E.J. et al. The immunological and oxidative stress regulation of non-thermal plasma-aided water on atopic dermatitis-like lesion in dinitrochlorobenzene-induced SKH-1 hairless mice. Mol. Cell. Toxicol. 15, 199–208 (2019). https://doi.org/10.1007/s13273-019-0023-y

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  • DOI: https://doi.org/10.1007/s13273-019-0023-y

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