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Fisetin protects H9c2 cardiomyoblast cells against H2O2-induced apoptosis through Akt and ERK1/2 signaling pathways

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Abstract

Backgrounds

Fisetin (tetrahydroxyflavone), a natural plant component, is known to have different properties including, direct radical scavenger, anti-inflammation, and cell survival.

Methods

We investigated whether fisetin can protect cardiomyocytes against H2O2-induced apoptosis that is relevant to cardiovascular disease risks using cell viability test, ROS measurement, and Western blotting.

Results

Fisetin decreased apoptotic cell death and intracellular reactive oxygen species (ROS), while enhancing the expression of Cu/Zn-superoxide dismutase (SOD) as well as phosphorylation of Akt and extracellular regulated kinase (ERK) 1/2. In particular, fisetin significantly decreased apoptosis through inhibition of cleaved caspase-3 and Bax expression and by enhancing the expression of anti-apoptotic enzyme as well as Bcl-2 in H2O2-stimulated H9c2 cells. However, the expression of heme oxygenase, catalase, and Mn-SOD was not altered by fisetin in these conditions.

Conclusion

Taken together, these data suggest that the potential cardioprotective effect of fisetin may involve Cu/Zn-SOD-mediated activation of Bcl-2 through the Akt and ERK1/2 pathways.

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Authors and Affiliations

  1. Department of Senior Healthcare, BK21 Plus Program, Graduate School, Eulji University, Daejeon, 34824, Republic of Korea

    Jeong Soo Lee, Kyung Jae Cha, Daye Lee & In Sik Kim

  2. Department of Clinical Laboratory Science, Wonkwang Health Science University, Iksan, 54538, Republic of Korea

    Ji-Sook Lee

  3. Department of Clinical Pathology, Sohae college, Gunsan, 54116, Republic of Korea

    Kyung Jae Cha

  4. Department of Biomedical Laboratory Science, Kyungbok University, Namyangju-si, Gyeonggi-do, 12051, Republic of Korea

    Dae-Eun Kim & Eun-Seok Park

  5. Department of Respiratory Medicine, School of Medicine, Eulji University, Daejeon, 34824, Republic of Korea

    Sun Young Jung

  6. Department of Biomedical Laboratory Science, School of Medicine, Eulji University, Daejeon, 34824, Republic of Korea

    In Sik Kim

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  1. Jeong Soo Lee
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Correspondence to Eun-Seok Park or In Sik Kim.

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Lee, J.S., Lee, JS., Cha, K.J. et al. Fisetin protects H9c2 cardiomyoblast cells against H2O2-induced apoptosis through Akt and ERK1/2 signaling pathways. Mol. Cell. Toxicol. 14, 183–192 (2018). https://doi.org/10.1007/s13273-018-0020-6

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  • DOI: https://doi.org/10.1007/s13273-018-0020-6

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