Molecular & Cellular Toxicology

, Volume 14, Issue 2, pp 183–192 | Cite as

Fisetin protects H9c2 cardiomyoblast cells against H2O2-induced apoptosis through Akt and ERK1/2 signaling pathways

  • Jeong Soo Lee
  • Ji-Sook Lee
  • Kyung Jae Cha
  • Dae-Eun Kim
  • Daye Lee
  • Sun Young Jung
  • Eun-Seok Park
  • In Sik Kim
Original Paper



Fisetin (tetrahydroxyflavone), a natural plant component, is known to have different properties including, direct radical scavenger, anti-inflammation, and cell survival.


We investigated whether fisetin can protect cardiomyocytes against H2O2-induced apoptosis that is relevant to cardiovascular disease risks using cell viability test, ROS measurement, and Western blotting.


Fisetin decreased apoptotic cell death and intracellular reactive oxygen species (ROS), while enhancing the expression of Cu/Zn-superoxide dismutase (SOD) as well as phosphorylation of Akt and extracellular regulated kinase (ERK) 1/2. In particular, fisetin significantly decreased apoptosis through inhibition of cleaved caspase-3 and Bax expression and by enhancing the expression of anti-apoptotic enzyme as well as Bcl-2 in H2O2-stimulated H9c2 cells. However, the expression of heme oxygenase, catalase, and Mn-SOD was not altered by fisetin in these conditions.


Taken together, these data suggest that the potential cardioprotective effect of fisetin may involve Cu/Zn-SOD-mediated activation of Bcl-2 through the Akt and ERK1/2 pathways.


Fisetin Oxidative stress Myocardial apoptosis Akt ERK1/2 


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Copyright information

© The Korean Society of Toxicogenomics and Toxicoproteomics and Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Jeong Soo Lee
    • 1
  • Ji-Sook Lee
    • 2
  • Kyung Jae Cha
    • 1
    • 3
  • Dae-Eun Kim
    • 4
  • Daye Lee
    • 1
  • Sun Young Jung
    • 5
  • Eun-Seok Park
    • 4
  • In Sik Kim
    • 1
    • 6
  1. 1.Department of Senior Healthcare, BK21 Plus Program, Graduate SchoolEulji UniversityDaejeonRepublic of Korea
  2. 2.Department of Clinical Laboratory ScienceWonkwang Health Science UniversityIksanRepublic of Korea
  3. 3.Department of Clinical PathologySohae collegeGunsanRepublic of Korea
  4. 4.Department of Biomedical Laboratory ScienceKyungbok UniversityNamyangju-si, Gyeonggi-doRepublic of Korea
  5. 5.Department of Respiratory Medicine, School of MedicineEulji UniversityDaejeonRepublic of Korea
  6. 6.Department of Biomedical Laboratory Science, School of MedicineEulji UniversityDaejeonRepublic of Korea

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