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Proteomic analysis of muscarinic acetylcholine receptor-mediated proliferation in HT-29 human colon cancer cells

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Abstract

Backgrounds

Muscarinic acetylcholine receptors (mAChRs) are members of G-protein-coupled receptors. They can induce agonist-dependent neoplastic transformation and facilitate colon cancer proliferation via promoting rapid expression of a variety of early responsive genes.

Methods

In this study, we used 2-dimensional gel electrophoresis (2-DE) approach with subsequent mass spectrometry (MS) to identify up- and down-regulated proteins (a total of 23 protein spots) involved in mAChRs-related signaling pathway, energy metabolism, transcription/translation, oxidative stress metabolism and cytoskeleton organization in agonist carbachol stimulated HT-29 human colon cells.

Results

We found that the increased expression of adenocarcinoma biomarker, annexin A5 (ANXA5) induced by carbachol treatment, which was confirmed by immunoblot. This study contributes to the understanding of mechanisms underlying mAChRs agonist-induced expression of whole proteins in HT-29 colon cancer cells.

Conclusion

Our results indicated that ANXA5 might serve as a potential biomarker for the diagnosis of colon cancer.

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Author information

Author notes

  1. Ar-Reum Lee

    Present address: Present Address: Department of New Drug Discovery and Development, Chungnam National University, Daejeon, 34134, Republic of Korea

  2. These authors contributed equally to this work.

Authors and Affiliations

  1. Gwangju Bio/Energy R&D Center, Korea Institute of Energy Research (KIER), 270-25 Samso-ro, Buk-gu, Gwangju, 61003, Republic of Korea

    Soo Youn Lee

  2. School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Republic of Korea

    Soo Youn Lee, Ar-Reum Lee, Ji-Young Ahn, Young-Chang Kim & Yang-Hoon Kim

  3. College of Veterinary Medicine, Western University of Health Sciences, 309 E Second Street, Pomona, CA, 91766, USA

    Jung Ho Ko, Lyon Lee & Janet Han

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  1. Soo Youn Lee
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  2. Ar-Reum Lee
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Corresponding authors

Correspondence to Young-Chang Kim or Yang-Hoon Kim.

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Lee, S.Y., Lee, AR., Ahn, JY. et al. Proteomic analysis of muscarinic acetylcholine receptor-mediated proliferation in HT-29 human colon cancer cells. Mol. Cell. Toxicol. 14, 155–162 (2018). https://doi.org/10.1007/s13273-018-0017-1

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  • DOI: https://doi.org/10.1007/s13273-018-0017-1

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