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Pre-labelled oligo probe-FISH karyotype analyses of four Araliaceae species using rDNA and telomeric repeat

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Abstract

Background

The family Araliaceae contains many medicinal species including ginseng of which the whole genome sequencing analyses have been going on these days.

Objective

To characterize the chromosomal distribution of 5S and 45S rDNAs and telomeric repeat in four ginseng related species of Aralia elata (Miq.) Seem., Dendropanax morbiferus H. Lév., Eleutherococcus sessiliflorus (Rupr. Et Maxim.) Seem., Kalopanax septemlobus (Thunb. ex A.Murr.) Koidz.

Method

Pre-labelled oligoprobe (PLOP)-fluorescence in situ hybridization (FISH) was carried out.

Results

The chromosome number of A. elata was 2n = 24, whereas that of the other three species of D. morbiferus, E. sessiliflorus, and K. septemlobus was 2n = 48, corresponding to diploid and tetraploid, respectively, based on the basic chromosome number x = 12 in Araliaceae. In all four species, one pair of 5S signals were detected in the proximal regions of the short arms of chromosome 3, whereas in K. septemlobus, the 5S rDNA signals localized in the subtelomeric region of short arm of chromosome 3, while all the 45S rDNA signals localized at the paracentromeric region of the short arm of chromosome 1. And the telomeric repeat signals were detected at the telomeric region of both short and long arms of most chromosomes.

Conclusion

The PLOP-FISH was very effective compared with conventional FISH method. These results provide useful comparative cytogenetic information to better understand the genome structure of each species and will be useful to trace the history of ginseng genomic constitution.

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Acknowledgements

This work was carried out with the support of the Cooperative Research Program for Agriculture Science & Technology Development (PJ01311902), Rural Development Administration, Republic of Korea.

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  1. Department of Life Sciences, Chromosome Research Institute, Sahmyook University, Seoul, 01795, Republic of Korea

    Hui Chao Zhou, Remnyl Joyce Pellerin, Nomar Espinosa Waminal & Hyun Hee Kim

  2. Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Republic of Korea

    Tae-Jin Yang

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Zhou, H.C., Pellerin, R.J., Waminal, N.E. et al. Pre-labelled oligo probe-FISH karyotype analyses of four Araliaceae species using rDNA and telomeric repeat. Genes Genom 41, 839–847 (2019). https://doi.org/10.1007/s13258-019-00786-x

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