Differential expression of primary pair-rule genes during bidirectional regeneration in Perionyx excavatus
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The earthworm Perionyx excavatus is a species highly capable of bidirectional regeneration. Pair-rule genes are thought to have an ancestral function in arthropod segmentation. However, orthologs in annelids (i.e. Capitella teleta) do not exhibit segmental expression in the ectoderm or mesoderm. Their role in regeneration is currently unclear. Here, we report the expression profile of primary pair-rule genes (Pex-EvxA, Pex-EvxB, Pex-RuntA, Pex-RuntB, Pex-Hes1A, Pex-Hes1B, Pex-Hes4A, and Pex-Hey) found in P. excavatus using semi-quantitative reverse transcription-polymerase chain reaction. Our results indicated these genes showed variable expression during bidirectional regeneration. Six of these genes might play diverse and potentially critical roles in head and/or tail regeneration.
KeywordsPair-rule genes Perionyx excavatus Bidirectional regeneration Earthworm
This work was supported by a Grant (CABX, No. PJ010001) from the Next-Generation BioGreen 21 Program funded by Rural Development Administration, Republic of Korea. This work was also supported by the research fund of Hanyang University (HY-2011-N to T.W.K), Republic of Korea.
Compliance with ethical standards
Conflict of interest
All authors, Yun-Sang Yu, Jin-Se Kim, Brenda Irene Medina Jiménez, Tae-Wuk Kim, and Sung-Jin Cho, declare that they have no conflict of interest.
This article does not contain any studies with human subjects or animals performed by any of the authors.
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