Identification and characterization of a novel goose-type and chicken-type lysozyme genes in Chinese rare minnow (Gobiocypris rarus) with potent antimicrobial activity

  • Yingying Zhang
  • Hui Yang
  • Weijia Song
  • Dan Cui
  • Lixin Wang
Research Article
  • 12 Downloads

Abstract

Lysozymes act as important innate immunity molecule against various pathogen infections. In the present study, a new c-type and g-type lysozymes were isolated from rare minnow Gobiocypris rarus. The deduced amino acid sequence of c-type lysozyme contained 145 residues and possessed conserved catalytic residues (Glu53 and Asp72). The deduced g-type lysozyme contained 185 residues and possessed three conserved catalytic residues (Glu73, Asp86 and Asp97). qPCR analysis revealed that GrlysG and GrlysC were constitutively expressed in all examined tissues. GrlysG was most abundant in kidney and liver, while GrlysC was predominantly expressed in liver. The transcripts of GrlysG and GrlysC genes could be significantly up-regulated after Aeromonas hydrophila and poly I:C infection in the kidney and liver. The recombinant GrlysG and GrlysC proteins were successfully produced and purified. The optimal pH and temperature for rGrlysG and rGrlysC protein lytic activities was determined to be 6.5 and 32 °C, respectively. From the minimal inhibitory concentration test, the rGrlysG and rGrlysC exhibited apparent antibacterial activities against both Gram-positive and Gram-negative bacteria at different concentrations. In conclusion, the present study indicated that c-type and g-type lysozymes participated the innate immune response to A. hydrophila and virus infections in fish.

Keywords

Rare minnow Lysozyme Expression analysis Lytic activity 

Notes

Acknowledgements

Financial support for this study was provided by Initial Research Foundation of Yangzhou University under Grant 137011293, Doctor Initial Research Foundation of Northwest A&F University under Grant Z109021640, the opening project of Scientific Observing and Experimental Station of the Ministry of Agriculture (2015SYKF-03).

Compliance with ethical standards

Conflict of interest

Yingying Zhang, Hui Yang, Weijia Song, Dan Cui and Lixin Wang declare that they have no conflict of interest.

Ethical approval

This article was approved by the Animal Ethics Committee of Northwest A&F University.

Supplementary material

13258_2018_656_MOESM1_ESM.docx (1.6 mb)
Supplementary material 1 (DOCX 1587 KB)

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Copyright information

© The Genetics Society of Korea and Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Yingying Zhang
    • 1
  • Hui Yang
    • 1
    • 2
  • Weijia Song
    • 2
  • Dan Cui
    • 2
  • Lixin Wang
    • 2
  1. 1.College of Animal Science and TechnologyYangzhou UniversityYangzhouChina
  2. 2.College of Animal Science and TechnologyNorthwest A&F UniversityYanglingChina

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