Fine mapping and candidate gene analysis of the quantitative trait locus gw8.1 associated with grain length in rice
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A quantitative trait locus (QTL) gw8.1 was detected in the population derived from a cross between the elite japonica cultivar, ‘Hwaseong’ and Oryza rufipogon (IRGC 105491). Near isogenic lines (NILs) harboring the O. rufipogon segment on chromosome 8 showed increased grain length and weight compared to those of the recurrent parent, Hwaseong. This QTL was mapped to a 175.3-kb region containing 28 genes, of which four were considered as candidates based on the presence of mutations in their coding regions and as per the RNA expression pattern during the inflorescence stage. Leaves and panicles obtained from plants harvested 5 days after heading showed differences in gene expression between Hwaseong and gw8.1-NILs. Most genes were upregulated in O. rufipogon and gw8.1-NIL than in Hwaseong. Scanning electron microscopy analysis of the lemma inner epidermal cells indicated that cell length was higher in gw8.1 NIL than in Hwaseong, indicating that gw8.1 might regulate cell elongation. Among the candidate genes, LOC_Os08g34380 encoding a putative receptor-like kinase and LOC_Os08g34550 encoding putative RING-H2 finger protein were considered as possible candidates based on their functional similarity.
KeywordsGrain weight Near isogenic line Oryza rufipogon Quantitative trait loci Rice
This work was carried out with the support of “Golden Seed Project” (Project No. PJ00993301), Ministry of Agriculture, Food and Rural Affairs (MAFRA), Ministry of Oceans and Fisheries (MOF), Rural Development Administration (RDA) and Korea Forest Service (KFS) and the Next-Generation Biogreen 21 Program for Agriculture &Technology Development (Project No. PJ01104801 to S.N.A), Rural Development Administration, Republic of Korea.
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Conflict of interest
Yun-Joo Kang, Kyu-Chan Shim, Hyun-Sook Lee, Yun-A Jeon, Sun-Ha Kim, Ju-Won Kang, Yeo-Tae Yun, In-Kyu Park, Sang-Nag Ahn declare that they have no conflict of interest.
Research involving human and animal participants
This article does not contain any studies with human subjects or animals performed by any of the authors.
- Cho YC, Suh JP, Choi IS, Hong HC, Baek MK, Kang KH, Kim YG, Ahn SN, Choi HC, Hwang HG, Moon HP (2003) QTLs analysis of yield and its related traits in wild rice relative Oryza rufipogon. Treatises Crop Res Korea 4:19–29Google Scholar
- Nagata K, Ando T, Nonoue Y, Mizubayashi T, Kitazawa N, Shomura A, Matsubara K, Ono N, Mizobuchi R, Shibaya T, Ogiso-Tanaka E, Hori K, Yano M, Fukuoka S (2015) Advanced backcross QTL analysis reveals complicated genetic control of rice grain shape in a japonica × indica cross. Breed Sci 65:308–318CrossRefPubMedPubMedCentralGoogle Scholar
- Takai T, Adachi S, Taguchi-Shiobara F, Sanoh-Arai Y, Iwasawa N, Yoshinaga S, Hirose S, Taniquchi Y, Yamanouchi U, Wu J, Matsumoto T, Sugimoto K, Kondo K, Ikka T, Ando T, Kono I, Ito S, Shomura A, Ookawa T, Hirasawa T, Yano M, Kondo M, Yamamoto T (2013) A natural variant of NAL1, selected in high-yield rice breeding programs, pleiotropically increases photosynthesis rate. Sci Rep. https://doi.org/10.1038/srep02149 PubMedPubMedCentralGoogle Scholar
- Yoon DB, Kang KH, Kim HJ, Ju HG, Kwon SJ, Suh JP, Jeong OY, Ahn SN (2006) Mapping quantitative trait loci for yield components and morphological traits in an advanced backcross population between Oryza grandiglumis and the O. sativa japonica cultivar Hwaseongbyeo. Theor Appl Genet 112:1052–1062CrossRefPubMedGoogle Scholar