Genes & Genomics

, Volume 40, Issue 4, pp 361–371 | Cite as

Selected microRNA-192 mutant indicates association with several function genes in bovine cells

  • Chen Zi
  • Dexin Zeng
  • Jiyong Zhou
  • Jianjun Dai
  • Luyan Jiang
  • Feng Xue
  • Yuan Jiang
  • Baoguang Li
Research Article


MicroRNAs are implicated in many cellular processes such as cell differentiation and development, tumorigenesis, and immune regulation. In this study, miR192 was detected using quantitative real-time polymerase chain reaction (qRT-PCR) when MDBK cells were exposed to Escherichia coli. Cells with malfunction of bta-miR-192 were established using transcription activator-like effector nuclease (TALEN) technology. Finally, bta-miR-192 mutant cells were screened for differentially expressed genes using RNA-sequencing (RNA-seq). The results showed that miR192 significantly decreased in cells exposed to E. coli F18ac and E. coli K88ac. The RNA-seq results showed that 1673 differentially expressed transcripts were identified; 890 genes were upregulated and 775 genes were downregulated. With the gene ontology enrichment analysis, 431 differentially expressed genes (DEGs) were classified into 937 gene ontology terms. The pathway enrichment analysis showed that 535 genes were involved in 254 pathway terms. Interestingly, most of these DEGs were associated with the pathways in cancers or infectious diseases. When the selected DEGs (n = 162) in these pathways were intersected with 120 differential transcripts, 11 DEGs were identified. Subsequently, several genes associated with regulation, cancers, or viral infections, such as LEF1, AXIN2, MX1, and FCGR2B, were identified among the DEGs using functional analysis. Furthermore, associations between bta-miR-192 and DEGs were detected by intersecting the bta-miR-192’s target genes with the DEGs, indicating that three genes including CBL, DICER1 and TRERF1 were involved in this relationship. These findings provided useful guidance for investigating the role played by bta-miR-192 in cellular functionality in bovine cells.


Bovine Differentially expressed genes Gene knockout MiR-192 RNA-seq 



This study was funded by the National Key Research and Development Program of China (2017YFF0208600), National “Youth Top-notch Talent” Support Program (W0270187), Introduction of Nanjing Agricultural University Scientific Research Grants Project (804121), the Scientific Research Project of Administration of Quality Supervision, Inspection, and Quarantine (2016IK131), the Science and Technology Program of Jiangsu Entry-Exit Inspection and Quarantine Bureau (2017KJ46), Central Guidance for Local Science and Technology Development (YDZX20173100004528), Science and Technology Joint Project of the Yangzte River Delta (17395810102), and the Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control.

Compliance with ethical standards

Conflict of interest

Chen Zi declares that he does not have a conflict of interest. Dexin Zeng declares that he does not have a conflict of interest. Jiyong Zhou declares that he does not have a conflict of interest. Jianjun Dai declares that he does not have a conflict of interest. Luyan Jiang declares that he does not have a conflict of interest. Feng Xue declares that he does not have a conflict of interest. Yuan Jiang declares that he does not have a conflict of interest. Baoguang Li declares that he does not have a conflict of interest.

Research involving human and animal participants

This article does not contain any studies with human participants or animals performed by any of the authors.


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Copyright information

© The Genetics Society of Korea and Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  • Chen Zi
    • 1
    • 2
  • Dexin Zeng
    • 1
  • Jiyong Zhou
    • 1
  • Jianjun Dai
    • 1
  • Luyan Jiang
    • 2
  • Feng Xue
    • 1
  • Yuan Jiang
    • 3
  • Baoguang Li
    • 4
  1. 1.MOE Joint International Research Laboratory of Animal Health and Food safety, College of Veterinary MedicineNanjing Agricultural UniversityNanjingChina
  2. 2.APFIC, Jiangsu Entry-Exit Inspection and Quarantine BureauNanjingChina
  3. 3.Shanghai Entry-Exit Inspection and Quarantine BureauShanghaiChina
  4. 4.Center for Food Safety and Applied NutritionU.S. Food and Drug AdministrationLaurelUSA

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