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In vivo evidence on the functional variation within fatty acid synthase gene associated with lipid metabolism in bovine longissimus dorsi muscle tissue

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Abstract

In Korean cattle, intramuscular fat (IMF), or marbling, of the longissimus dorsi muscle (LM) cross section is one of the most important indicators of beef quality and are influenced by environmental and genetic factors. This study was to evaluate the effect of SNPs on the beef quality in Korean cattle for functional studies, such as site-directed mutagenesis based on bovine adipocytes. The fatty acid synthase (FASN) gene plays an important role in lipogenesis. FASN is an essential metabolic and multifunctional enzyme in fatty acid synthesis. Several studies have reported that SNPs g.841G, g.16024A, g.16039T, and g.17924G have a significant impact on marbling scores in Korean cattle and Japanese Black cattle population. These SNPs are located in transcription factor binding sites, the beta-ketoacyl reductase, and thioesterase domains. Our results revealed that the g.17924 A>G SNP is located in the thioesterase domain of the FASN protein, and changes from polar, neutral, and hydrophilic to nonpolar, aliphatic, and hydrophobic, respectively. In in vivo LM tissue of Korean cattle, the g.17924A>G SNP has an effect on increasing fat deposition. Therefore, g.17924A>G SNP could be a causal mutation for increasing fat deposition in Korean cattle LM tissue.

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Acknowledgements

This work was supported by a grant from the Next-Generation BioGreen 21 Program (No. PJ011140), Rural Development Administration, Republic of Korea.

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    Authors and Affiliations

    1. Livestock Research Institute, Gyeongsangbuk-Do, Yeongju, Gyeongbuk, 36052, Republic of Korea

      Dong-yep Oh & Jaejung Ha

    2. Research Center for Environment Friendly and Quality Livestock Production Technology, Hankyong National University, Anseong, Gyeonggi, 17579, Republic of Korea

      Insik Nam

    3. Department of Biotechnology, College of Agriculture & Life Science, Hankyong National University, Anseong, Gyeonggi, 17579, Republic of Korea

      Sehwan Hwang, Hongsik Kong & Yoonseok Lee

    4. Department of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University, Seoul, 05029, Republic of Korea

      Honggu Lee

    5. Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Republic of Korea

      Myunggi Baik

    6. Department of Nanobiomedical Science, Dankook University, Cheonan, 31116, Republic of Korea

      Man Hwan Oh, Songmi Kim & Kyudong Han

    7. BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, 31116, Republic of Korea

      Songmi Kim & Kyudong Han

    Authors
    1. Dong-yep Oh
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    Correspondence to Kyudong Han or Yoonseok Lee.

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    Conflict of interest

    Dong-yep Oh declares that he has no conflict of interest. Insik Nam declares that he has no conflict of interest. Sehwan Hwang declares that he has no conflict of interest. Hongsik Kong declares that he has no conflict of interest. Honggu Lee declares that he has no conflict of interest. Jaejung Ha declares that he has no conflict of interest. Myunggi Baik declares that he has no conflict of interest. Man Hwan Oh declares that he has no conflict of interest. Songmi Kim declares that she has no conflict of interest. Kyudong Han declares that he has no conflict of interest. Yoonseok Lee declares that he has no conflict of interest.

    Ethical approval

    All research protocols and animal experiments in this study were reviewed and approved by the Institutional Animal Care and Use Committee (IACUC) in Gyeongsangbuk-do, Republic of Korea (Gyeongbuk IACUC-87).

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    Dong-yep Oh and Insik Nam contributed equally to this work.

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    Oh, Dy., Nam, I., Hwang, S. et al. In vivo evidence on the functional variation within fatty acid synthase gene associated with lipid metabolism in bovine longissimus dorsi muscle tissue. Genes Genom 40, 289–294 (2018). https://doi.org/10.1007/s13258-017-0634-4

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    • DOI: https://doi.org/10.1007/s13258-017-0634-4

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