The Nucleus

, Volume 62, Issue 1, pp 77–82 | Cite as

Chromosome studies on freshwater prawn, Macrobrachium lanchesteri (Decapoda, Palaemonidae) from Thailand

  • Sumalee Phimphan
  • A. Tanomtong
  • N. seangphan
  • W. SangpakdeeEmail author


The chromosomes of freshwater prawn (Macrobrachium lanchesteri) were characterized by means of conventional Giemsa-, DAPI-, silver-staining and G-banding techniques. The number of diploid chromosome and fundamental number were 2n = 116 and 226, respectively. The karyotype consisted of 54 metacentric, 46 submetacentric, 10 acrocentric and 6 telocentric chromosome pairs. The karyotype formula could be deduced as 2n (116) = L 24 m  + L 8 a  + M 12 m  + M 6 sm  + M 2 a  + S 18 m  + S 40 sm  + S 6 t . Heterochromatic regions were located in the centromere and pericentromeric regions of most chromosomes, and NORs were found on two pairs of small chromosomes. G-band karyotype of M. lanchesteri was provided for the first time.


Freshwater prawn Macrobrachium lanchesteri Karyotype 



The authors are grateful to Udon Thani Rajabhat University, Phetchabun Rajabhat University and the Toxic Substances in Livestock and Aquatic Animals Research Group, Khon Kaen University for financial support and collaboration. The authors are also thanks to Suphanburi College of Agriculture and Technology, Amphoe Danchang Suphanburi Province for identifying the specimens.


  1. 1.
    Anastasiadou C, Leonardos ID. Karyological analysis of the freshwater shrimp Atyaephyra desmarestii (Decapoda: Caridea: Atyidae). J Crust Biol. 2010;30:332–5.CrossRefGoogle Scholar
  2. 2.
    Burkenroad MD. The templeton crocker expedition. XIII. Penaeidae from the region of lower California and Clarion Island, with descriptions of four new species. Zoologica. 1938;233:55–91.Google Scholar
  3. 3.
    Cai Y, Naiyanetr P, Ng PKL. The freshwater prawns of the genus Macrobrachium Bate, 1868 of Thailand (Crustacea: Decapoda: Palaemonidae). J Nat Hist. 2004;38:581–649.CrossRefGoogle Scholar
  4. 4.
    Campos-Ramos R. Chromosome studies on the marine shrimps Penaeus vannamei and P. californiensis (Decapoda). J Crust Biol. 1997;17:666–73.CrossRefGoogle Scholar
  5. 5.
    Chaiyasut K. Cytogenetics and cytotaxonomy of the family Zephyranthes. Bangkok: Department of Botany, Faculty of Science, Chulalongkorn University; 1989.Google Scholar
  6. 6.
    Chavez Justo C, Murofushi M, Aida K, Hanyu I. Karyological studies on the freshwater prawn Macrobrachium rosenbergii. Aquaculture. 1991;97:327–34.CrossRefGoogle Scholar
  7. 7.
    Chong SSC, Khoo HW. The identity of Macrobrachium lanchesteri (De Man, 1911) (Decapoda, Palaemonidae) from Peninsular Malaysia and Singapore, and a description of its first zoea. Crustaceana. 1988;54:196–206.CrossRefGoogle Scholar
  8. 8.
    Chow S, Dougherty WJ, Sandife PA. Meiotic chromosome complements and nuclear DNA contents of four species of shrimps of the genus Penaeus. J Crust Biol. 1990;10:29–36.CrossRefGoogle Scholar
  9. 9.
    De Man JG. On the west-African species of the subgenus Eupalaemon Ortm. Zool Meded. 1991;33:261–4.Google Scholar
  10. 10.
    Diupotex Chong ME, Foster NR, Zarate LA. A cytogenetic study of the crayfish Procambarus digueti (Bouvier, 1897) (Decapoda Cambaridae) from Lake Camecuaro, Michoacan, Mexico. Crustaceana. 1997;70:875–85.CrossRefGoogle Scholar
  11. 11.
    Fasten N. Spermatogenesis of the American crayfish, Cambarus virilis and C. immunis (?), with special reference to synapsis and the chromatoid bodies. J Morphol. 1914;25:587–649.CrossRefGoogle Scholar
  12. 12.
    Gersen SL, Keagle MB. The principles of clinical cytogenetics. New York: Springer; 2013.CrossRefGoogle Scholar
  13. 13.
    Howell WM, Black DA. Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia. 1980;36:1014–5.CrossRefGoogle Scholar
  14. 14.
    Indy JR, Arias-Rodriguez L, Páramo-Delgadillo S, Hernández-Vidal U, Alvarez-González CA, Contreras-Sánchez WM. Mitotic karyotype of the tropical freshwater crayfish Procambarus (Austrocambarus) llamasi (Decapoda: Cambaridae). Rev Biol Trop. 2010;58:655–62.Google Scholar
  15. 15.
    Johnson DS. A synopsis of the Decapoda Caridea and Stenopodidea of Singapore, with notes on their distribution and a key to genera of Caridea occurring in Malayan waters. Bull Natl Mus Singap. 1961;30:44–79.Google Scholar
  16. 16.
    Johnson DS. Biology of potentially valuable fresh-water prawns with special reference to the Riceland prawn Cryphiops (Macrobrachium) lanchesteri (De Man). FAO Fish Rep. 1968;57:233–41.Google Scholar
  17. 17.
    Kampiranont A. Cytogenetics. Bangkok: Department of Genetics, Faculty of Science, Kasetsart University; 2003.Google Scholar
  18. 18.
    Lakra WS, Kumar P, Das M, Goswami U. Improved techniques of chromosome preparation from shrimp and prawn. Asian Fish Sci. 1997;10:117–21.Google Scholar
  19. 19.
    Lanchester WF. On the Crustacea collected during the ‘Skeat’ expedition to the Malay Peninsula. Proc Zool Soc London. 1901;2:533–74.Google Scholar
  20. 20.
    Lécher P, Defaye D, Noel P. Chromosomes and nuclear DNA of Crustacea. Invertebr Reprod Dev. 1995;27:85–114.CrossRefGoogle Scholar
  21. 21.
    Liehr T. Fluorescence in situ hybridization (FISH) application guide. Heidelberg: Springer; 2009.CrossRefGoogle Scholar
  22. 22.
    Mittal OP, Dhall U. Chromosome studies in three species of freshwater decapods (Crustacea). Cytologia. 1971;36:633–8.CrossRefGoogle Scholar
  23. 23.
    Morelli M, Le Dean L, Vonau V, Diter A. Karyotype of the marine shrimp Penaeus indicus (Crustacea Decapoda) established by using an image analysis system. Ophelia. 1998;49:83–95.CrossRefGoogle Scholar
  24. 24.
    Murofushi M, Deguchi Y, Yoshida H. Karyological study of the red swamp crayfish and the Japanese lobster by air-drying method. Proc Jpn Acad. 1984;60:306–9.CrossRefGoogle Scholar
  25. 25.
    Naiyanetr P. Checklist of Crustacean Fauna in Thailand (Decapoda, Stomatopoda, Anostraca, Myodacopa and Isopoda). Bangkok: Office of Natural Resources and Environmental Policy and Planning; 2007. p. 33–9.Google Scholar
  26. 26.
    Ng PKL. On a collection of freshwater decapoda crustaceans from the Kinabatangan River, Sabah, Malaysia, with descriptions of three new species. Sabah Muse. 1994;1:73–92.Google Scholar
  27. 27.
    Niiyama H. The chromosomes of the crayfish Cambaroides japonicus (de Haan). J Fac Sci Hokkaido Univ Zool. 1934;3:41–53.Google Scholar
  28. 28.
    Niiyama H. The chromosomes of a Japanese spiny lobster, Panulirus japonicus (v. Siebold). J Fac Sci Hokkaido Univ Zool. 1936;5:21–8.Google Scholar
  29. 29.
    Niiyama H. A comparative study of the chromosomes in decapods, isopods and amphipods, with some remarks on cytotaxonomy and sex-determination in Crustacea. Mem Fac Fish Hokkaido Univ. 1959;7:1–60.Google Scholar
  30. 30.
    Niiyama H. On the unprecedentedly large number of chromosomes of the crayfish, Astacus trowbridgii. Stimpson Annot Zool Jpn. 1962;35:229–33.Google Scholar
  31. 31.
    Pavlica M, Mlinarec J, Muzic M, Maguire I, Srut M, Klobucar GIV. Karyological analysis of three Astacidae species a preliminary report. In: 17th symposium of international association of astacology, Kuopio, Finland; 2008; vol. 8: p. 4–8.Google Scholar
  32. 32.
    Saengphan N, Suksawad B, Suksomnid A, Chalueisak P. Freshwater shrimp in Thailand and potential using. Bangkok: Complete Report for Research and Development Center, Office of the Vocational Education Commission; 2016. p. 34.Google Scholar
  33. 33.
    Salvadori S, Coluccia E, Deidda F, Cau A, Cannas R, Lobina C, Sabatini A, Deiana AM. Karyotype, ribosomal genes, and telomeric sequences in the Crayfish Procambarus Clarkii (Decapoda: Cambaridae). J Crust Biol. 2014;34:525–31.CrossRefGoogle Scholar
  34. 34.
    Scalici M, Solano E, Gibertini G. Karyological analyses on the Australian crayfish Cherax destructor (Decapoda: Parastacidae). J Crust Biol. 2010;30:528–30.CrossRefGoogle Scholar
  35. 35.
    Sharma OP, Tripathi NK, Sharma KK. A review of chromosome banding in fishes. In: Sobti RC, editor. Some aspects of chromosome structure and functions. New Delhi: Narosa Publishing House; 2002.Google Scholar
  36. 36.
    Suvatti C. A check-list of aquatic fauna in Siam (Excluding Fishes). Bangkok: Buraeu of Fisheries; 1937. p. 1–116.Google Scholar
  37. 37.
    Tan X, Qin JG, Chen B, Chen L, Li X. Karyological analyses on redclaw crayfish Cherax quadricarinatus (Decapoda: Parastacidae). Aquaculture. 2004;234:65–76.CrossRefGoogle Scholar
  38. 38.
    Vishnoi DN. Studies on the chromosomes of some Indian Crustacea. Cytologia. 1972;37:43–51.CrossRefGoogle Scholar
  39. 39.
    Wong JTY. Larval development of the Palaemonid prawn, Macrobrachium lanchesteri (De Man) reared in the laboratory (Decapoda, Caridea). Crustaceana. 1994;67:297–315.CrossRefGoogle Scholar
  40. 40.
    Xiang JH, Liu RY, Zhou LH. Chromosomes of marine shrimps with special reference to different techniques. Aquaculture. 1994;111:321.CrossRefGoogle Scholar

Copyright information

© Archana Sharma Foundation of Calcutta 2018

Authors and Affiliations

  • Sumalee Phimphan
    • 1
  • A. Tanomtong
    • 2
  • N. seangphan
    • 3
  • W. Sangpakdee
    • 4
    Email author
  1. 1.Biology Program, Faculty of Science and TechnologyPhetchabun Rajabhat UniversityMuangThailand
  2. 2.Department of Biology, Faculty of ScienceKhon Kaen UniversityMuangThailand
  3. 3.Suphanburi College of Agriculture and TechnologyDanchangThailand
  4. 4.Biology Program, Faculty of ScienceUdon Thani Rajabhat UniversityMuangThailand

Personalised recommendations