Abstract
The nucleus of every cell is arranged in a manner that reflects the cell’s functional identity. Its DNA loops around histones that help compact and regulate the local functional state. Various other proteins along with RNAs are arranged along the DNA. Barrier and insulator elements partition it into functional elements. Other proteins interact with their binding sites, recruit other factors, to enable the modifying of histones according to the functional state it must be in. Depending on this state, other proteins can facilitate transcription, repair, replication etc. To this end, the study of the spatial distribution of these proteins and RNAs is necessary to understand their functional relevance within the context of the nucleus. Here, we review various established and emerging techniques that enable such studies.
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TP acknowledges the Council of Scientific and Industrial Research (CSIR) for doctoral fellowship and RKM acknowledges the financial support of the CSIR.
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This article is dedicated in memory of Profs AK Sharma and Archana Sharma.
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Ponrathnam, T., Mishra, R.K. From chromosomes to genomes: new insights with emerging techniques. Nucleus 61, 227–234 (2018). https://doi.org/10.1007/s13237-018-0242-y
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DOI: https://doi.org/10.1007/s13237-018-0242-y