Abstract
Flax (Linum usitatissimum L., 2n = 30), is an annual self-pollinated crop. It is used in oil and fiber industries, and also valued for pharmaceutical and nutraceutical applications. Characterization of flax gene pool and evaluation of genetic diversity are crucial for germplasm conservation and breeding. In present study, eighteen retrotransposon microsatellite amplified polymorphism markers were used to evaluate the genetic diversity of cultivated flax within and among populations. Thirty samples of flax plants from three different climates and geographic regions in Iran were used for present investigation. Analysis of molecular variance test showed significant genetic difference (PhiPT = 0.595, P = 0.001) among the studied populations in L. usitatissimum. 41% of total genetic variability was due to within population diversity, whereas 59% was due to among population genetic differentiation. The PCoA plot and cluster analysis grouped plants of Saveh population in a cluster entirely separated from the other two populations and showed high genetic affinity between the two Shiraz and Orumieh populations. STRUCTURE plot identified two distinct gene pools for flax, so that the population of Saveh is different from other populations, but some degree of shared alleles occurred between them.
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Bickel CL, Gadani S, Lukacs M, Cullis CA. SSR markers developed for genetic mapping in flax (Linum usitatissimum L.). Res Rep Biol. 2011;2:23–9.
Biswas MK, Xu Q, Deng XX. Utility of RAPD, ISSR, IRAP and REMAP markers for the genetic analysis of Citrus spp. Sci Hort. 2010;124:254–61.
Caliński T, Harabasz J. A dendrite method for cluster analysis. Commun Stat Theory Methods. 1974;3:1–27.
Chen HH, Xu SY, Wang Z. Gelation properties of flaxseed gum. J Food Eng. 2006;77:295–303.
Cloutier S, Miranda E, Ward K, Radovanovic N, Reimer E, Walichnowski A, et al. Simple sequence repeat marker development from bacterial artificial chromosome end sequences and expressed sequence tags of flax (Linum usitatissimum L.). Theor Appl Genet. 2012;125:685–94.
Cullis CA. Mechanisms and control of rapid genomic changes in flax. Ann Bot. 2005;95:201–6.
Deng X, Long S, He D, Li X, Wang Y, Hao D, et al. Isolation and characterization of polymorphic microsatellite markers from flax (Linum usitatissimum L.). Afr J Biotechnol. 2011;10:734–9.
Evanno G, Regnaut S, Goudet J. Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol. 2005;14:2611–20.
Flavell AJ, Smith DB, Kumar A. Extreme heterogeneity of Ty1-copia group retrotransposons in plants. Mol Gen Genet. 1992;231:233–42.
Freeland JR, Kirk H, Peterson SD. Molecular ecology. Hoboken: Wiley; 2011. p. 449.
Fu YB. Redundancy and distinctness in flax germplasm as revealed by RAPD dissimilarity. Plant Genet Resour. 2006;4:117–24.
González LG, Deyholos MK. Identification, characterization and distribution of transposable elements in the flax (Linum usitatissimum L.) genome. BMC Genom. 2012;13:644.
Grandbastien MA, Lucas H, Morel JB, Mhiri C, Vernhettes S, Casacuberta JM. The expression of the tobacco Tnt1 retrotransposon is linked to plant defense responses. Genetica. 1997;100:241–52.
Habibollahi H, Noormohammadi Z, Sheidai M, Farahani F. SSR and EST-SSR-based population genetic structure of Linum L. (Linaceae) species in Iran. Genet Resour Crop Evol. 2016;63:1127–38.
Habibollahi H, Noormohammadi Z, Sheidai M, Farahani F. Genetic structure of cultivated flax (Linum usitatissimum L.) based on retrotransposon-based markers. Genetika. 2015;47:1111–22.
Huson DH, Bryant D. Application of phylogenetic networks in evolutionary studies. Mol Biol Evol. 2006;23:254–67.
Hutchison DW, Templeton AR. Correlation of pairwise genetic and geographic distance measures: inferring the relative influences of gene flow and drift on the distribution of genetic variability. Evolution. 1999;53:1898–914.
Jenab M, Thompson LU. The influence of flaxseed and lignans on colon carcinogenesis and β-glucuronidase activity. Carcinogenesis. 1996;17:1343–8.
Kalendar R, Schulman AH. IRAP and REMAP for retrotransposon-based genotyping and fingerprinting. Nat Protoc. 2006;1:2478–84.
Kalendar R, Grob T, Regina M, Suoniemi A, Schulman A. IRAP and REMAP: two new retrotransposon-based DNA fingerprinting techniques. Theor Appl Genet. 1999;98:704–11.
Križman M, Jakše J, Baričevič D, Javornik B, Prošek M. Robust CTAB-activated charcoal protocol for plant DNA extraction. Acta Agric Slov. 2006;87:427–33.
Leigh F, Kalendar R, Lea V, Lee D, Donini P, Schulman AH. Comparison of the utility of barley retrotransposon families for genetic analysis by molecular marker techniques. Mol Genet Genomics. 2003;269:464–74.
Meirmans PG. AMOVA-based clustering of population genetic data. J Hered. 2012;103:744–50.
Meirmans PG, Van Tienderen PH. GENOTYPE and GENODIVE: two programs for the analysis of genetic diversity of asexual organisms. Mol Ecol Notes. 2004;4:792–4.
Pritchard JK, Stephens M, Donnelly P. Inference of population structure using multilocus genotype data. Genetics. 2000;155:945–59.
Provan J, Thomas WT, Forster BP, Powell W. Copia-SSR: a simple marker technique which can be used on total genomic DNA. Genome. 1999;42:363–6.
SanMiguel P, Tikhonov A, Jin YK, Motchoulskaia N. Nested retrotransposons in the intergenic regions of the maize genome. Science. 1996;274:765.
Setsuko S, Ishida K, Ueno S, Tsumura Y, Tomaru N. Population differentiation and gene flow within a metapopulation of a threatened tree, Magnolia stellata (Magnoliaceae). Am J Bot. 2007;94:128–36.
Sheidai M, Afshar F, Keshavarzi M, Talebi SM, Noormohammadi Z, Shafaf T. Genetic diversity and genome size variability in Linum austriacum (Lineaceae) populations. Biochem Sys Ecol. 2014;57:20–6.
Sheidai M, Seif E, Nouroozi M, Noormohammadi Z. Cytogenetic and molecular diversity of Cirsium arvense (Asteraceae) populations in Iran. J Jpn Bot. 2012;87:193–205.
Sheidai M, Zanganeh S, Haji-Ramezanali R, Nouroozi M, Noormohammadi Z, Ghsemzadeh-Baraki S. Genetic diversity and population structure in four Cirsium (Asteraceae) species. Biologia. 2013;68:384–97.
Shirasu K, Schulman AH, Lahaye T, Schulze-Lefert P. A contiguous 66-kb barley DNA sequence provides evidence for reversible genome expansion. Genome Res. 2000;10:908–15.
Smýkal P, Bačová-Kerteszová N, Kalendar R, Corander J, Schulman AH, Pavelek M. Genetic diversity of cultivated flax (Linum usitatissimum L.) germplasm assessed by retrotransposon-based markers. Theor Appl Genet. 2011;122:1385–97.
Soto-Cerda BJ, Maureira-Butler I, Muñoz G, Rupayan A, Cloutier S. SSR-based population structure, molecular diversity and linkage disequilibrium analysis of a collection of flax (Linum usitatissimum L.) varying for mucilage seed-coat content. Mol Breed. 2012;30:875–88.
Soto-Cerda BJ, Carrasco RA, Aravena GA, Urbina HA, Navarro CS. Identifying novel polymorphic microsatellites from cultivated flax (Linum usitatissimum L.) following data mining. Plant Mol Biol Rep. 2011;29:753–9.
Suoniemi A, Tanskanen J, Schulman AH. Gypsy-like retrotransposons are widespread in the plant kingdom. Plant J. 1998;13:699–705.
Van Zeist W, Bakker-Heeres JA. Evidence for linseed cultivation before 6000 BC. J Archaeol Sci. 1975;2:215–9.
Voytas DF, Cummings MP, Koniczny A, Ausubel FM, Rodermel SR. Copia-like retrotransposons are ubiquitous among plants. Proc Nat Acad Sci. 1992;89:7124–8.
Wang Z, Hobson N, Galindo L, Zhu S, Shi D, McDill J, et al. The genome of flax (Linum usitatissimum) assembled de novo from short shotgun sequence reads. Plant J. 2012;72:461–73.
Zohary D, Pulses Hopf M. Domestication of plants in the old world: the origin and spread of cultivated plants in West Asia, Europe, and the Nile Valley. Oxford: Oxford Press; 2000. p. 316.
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We gratefully acknowledge Mr. Reza Assareh and Mrs. Bahareh Ghasemzadeh for their aids in Science and Research Branch of Islamic Azad University and Shahid Beheshti University.
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Habibollahi, H., Noormohammadi, Z., Sheidai, M. et al. Assessments of genetic diversity in Iranian flax populations using retrotransposon microsatellite amplification polymorphisms (REMAP) markers. Nucleus 61, 55–60 (2018). https://doi.org/10.1007/s13237-017-0218-3
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DOI: https://doi.org/10.1007/s13237-017-0218-3