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The Nucleus

, Volume 61, Issue 1, pp 29–34 | Cite as

Characterization of Tulbaghia violacea (Tulbaghieae, Allioideae, Amaryllidaceae) from India: a cytogenetic and molecular approach

  • Abhishek Sadhu
  • Sreetama Bhadra
  • Maumita Bandyopadhyay
Original Article
  • 117 Downloads

Abstract

Tulbaghia violacea, called ‘wild garlic’, is a native of Africa, though it is widely cultivated all over the world for its medicinal and horticultural importance. Absence of the genus from Indian floras and taxonomic surveys, as well as lack of proper molecular and cytogenetic characterizations, have instigated questions regarding origin and divergence of the Indian counterpart from its African relatives. In the present study, morphotaxonomical identification of Indian T. violacea was carried out, along with its karyomorphological characterization, 2C nuclear genome content determination, and analysis of ITS sequence. Mitotic study revealed that this species possessed 2n = 2x = 12 somatic chromosomes, two of which had secondary constrictions, whereas unstable meiotic chromosomal behavior was observed in flower buds, as reported earlier. 2C nuclear DNA content of T. violacea (44.51 ± 0.06 pg) was reported for the first time using flow cytometry, as was the ITS sequence data, from India.

Keywords

Tulbaghia violacea Chromosome DNA content Internal transcribed spacer 

Notes

Acknowledgements

Authors acknowledge the Young Scientist Project, Science and Engineering Research Board, Department of Science and Technology, Government of India, for financial assistance. Dr. Jaroslav Doležel (Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Czech Republic) is acknowledged for his kind contribution with flow cytometry standard plant seeds. The Director, Centre for Research in Nanoscience & Nanotechnology (CRNN), University of Calcutta is acknowledged for providing BD FACSVerse™ Flow Cytometer instrument used in this study.

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Copyright information

© Archana Sharma Foundation of Calcutta 2017

Authors and Affiliations

  • Abhishek Sadhu
    • 1
  • Sreetama Bhadra
    • 1
  • Maumita Bandyopadhyay
    • 1
  1. 1.Plant Molecular Cytogenetics Laboratory, Centre of Advanced Study, Department of BotanyUniversity of CalcuttaKolkataIndia

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