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Comparative karyomorphological analyses of in vitro and in vivo grown plants of Aloe vera L. BURM. f.

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Abstract

Aloe vera L. Burm. f. is used as a popular folk medicine with many cultivars growing naturally or in cultivation in different parts of the world. The present report describes the methods of in vitro propagation and conservation as well as detailed chromosomal analysis of two morphologically indistinguishable cultivars of Aloe vera obtained from NBPGR at the CAZRI (Central Arid Zone Research Institute), Jodhpur, India. The study shows the highly conserved orthoselected karyotype in Aloe vera both in in vivo and in vitro grown cultivars. Cytological investigation of one in vitro raised plant revealed its tetraploid nature. Fourteen chromosomes were resolved into 7 bivalents during meiosis of both the cultivars. Percentage of pollen viability is high in both the cultivars yet the flowers failed to form fruits. Pollen mitosis was used to prepare haploid karyotypes in our study.

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Acknowledgements

Authors are thankful to NBPGR, CAZRI, Jodhpur, India for providing the plant material and to the Plant Biotechnology Laboratory, Department of Botany, Presidency College, Kolkata for providing necessary funds and facilities.

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Authors and Affiliations

  1. Department of Botany, Plant Biotechnology Laboratory, Presidency College, Kolkata, 700073, West Bengal, India

    Anusree Das, Priyanka Mukherjee, Animesh Ghorai & Timir baran Jha

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  1. Anusree Das
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  2. Priyanka Mukherjee
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Correspondence to Timir baran Jha.

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Das, A., Mukherjee, P., Ghorai, A. et al. Comparative karyomorphological analyses of in vitro and in vivo grown plants of Aloe vera L. BURM. f.. Nucleus 53, 89–94 (2010). https://doi.org/10.1007/s13237-010-0001-1

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