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Microbiological, biochemical, physicochemical surface properties and biofilm forming ability of Brettanomyces bruxellensis

  • Maria DimopoulouEmail author
  • Margareth Renault
  • Marguerite Dols-Lafargue
  • Warren Albertin
  • Jean-Marie Herry
  • Marie-Noëlle Bellon-Fontaine
  • Isabelle Masneuf-Pomarede
Original Article
  • 94 Downloads

Abstract

Purpose

Brettanomyces bruxellensis is a serious source of concern for winemakers. The production of volatile phenols by the yeast species confers to wine unpleasant sensory characteristics which are unacceptable by the consumers and inevitably provoke economic loss for the wine industry. This ubiquitous yeast is able to adapt to all winemaking steps and to withstand various environmental conditions. Moreover, the ability of B. bruxellensis to adhere and colonize inert materials can be the cause of the yeast persistence in the cellars and thus recurrent wine spoilage. We therefore investigated the surface properties, biofilm formation capacity, and the factors which may affect the attachment of the yeast cells to surfaces with eight strains representative of the genetic diversity of the species.

Methods

The eight strains of B. bruxellensis were isolated from different geographical and industrial fermentation origins. The cells were grown in synthetic YPD medium containing 1% (w/v) yeast extract (Difco Laboratories, Detroit), 2% (w/v) bacto peptone (Difco), and 1% (w/v) glucose. Surface physicochemical properties as electrophoretic mobility and adhesion to hydrocarbon of the cells were studied. The ability of the strains to form biofilm was quantified using a colorimetric microtiter 96-well polystyrene plate. Biochemical characteristics were examined by colorimetric methods as well as by chemical analysis.

Result

Our results show that the biofilm formation ability is strain-dependent and suggest a possible link between the physicochemical properties of the studied strains and their corresponding genetic group.

Conclusion

The capacity to detect and identify the strains of the spoilage yeast based on their biofilm formation abilities may help to develop more efficient cleaning procedures and preventing methods.

Keywords

Brettanomyces bruxellensis Wine spoilage Biochemical properties Physico-chemical surface properties Biofilm formation 

Notes

Funding

This work was supported by funds from FranceAgriMer. Lipids analysis was performed at the lipidomic plateform of Bordeaux University.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This work does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

Informed consent was obtained from all individual participants included in the study. The study has been also published as a preprint version with doi  https://doi.org/10.1101/579144.

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Copyright information

© Università degli studi di Milano 2019

Authors and Affiliations

  • Maria Dimopoulou
    • 1
    Email author
  • Margareth Renault
    • 2
  • Marguerite Dols-Lafargue
    • 1
    • 3
  • Warren Albertin
    • 1
    • 3
  • Jean-Marie Herry
    • 2
  • Marie-Noëlle Bellon-Fontaine
    • 2
  • Isabelle Masneuf-Pomarede
    • 1
    • 4
  1. 1.UR Oenologie EA 4577, USC 1366 INRA, Bordeaux INPUniversité de BordeauxBordeauxFrance
  2. 2.UMR Génie et Microbiologie des Procédés Alimentaires, GMPA, AgroParisTech, INRAUniversité Paris-SaclayThiverval-GrignonFrance
  3. 3.ENSCBP, Bordeaux INPPessacFrance
  4. 4.Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-AquitaineBordeauxFrance

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