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Identification and characterization of ectoine-producing bacteria isolated from Can Gio mangrove soil in Vietnam

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A Correction to this article was published on 16 November 2019

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Abstract

Purpose

The aim of this study was to characterize high ectoine-producing bacteria obtained from Can Gio mangrove soil samples in Vietnam.

Methods

Ectoine-producing bacteria were isolated from mangrove soil samples. The selected strains were identified using 16S rDNA sequence analysis, and their biochemical characteristics were also examined. The ability to produce ectoine at different NaCl concentrations and the effect of osmotic downshock solution on ectoine’s release rates and survival rates for the selected bacterial strains were investigated.

Results

Among more than 200 bacterial colonies isolated from soil samples, two strains exhibiting highest ectoine production (strains D227 and D228) were chosen for further studies. Both strains D227 and D228 were identified as Halomonas spp. and were closely related to Halomonas organivorans, sharing 99.4% 16S rDNA sequence similarity. At 6% (w/v) NaCl concentration, strains D227 and D228 presented the highest cell dry weight (CDW) of 3.85 and 3.55 g/l, respectively. At 18% NaCl concentration, maximum total ectoine (ectoine and hydroxyectoine) production of 16.4 and 18.1 wt% was achieved by strains D227 and D228, respectively. After 30 min of incubation in downshock solution containing 5% NaCl, high bacterial survival rates of 96% and 98%, and ectoines release rates of 61% and 76% were obtained by strains D227 and D228, respectively.

Conclusions

The accumulation and secretion of ectoine appear to be a typical adaptation strategy of some bacteria to survive under the changing saline conditions of mangrove ecosystem. To the best of our knowledge, this is the first report on ectoine production by halophilic bacteria isolated from mangrove soil. High ectoine-producing bacteria can be found in mangrove forest.

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Change history

  • 16 November 2019

    After publication of this paper, the authors determined an error in the funding information. It was written as Grant B2007-SHP-32, but it should be Grant B2017-SPH-32.

References

  • Borges N, Ramos A, Raven NDH, Sharp RJ (2002) Comparative study of the thermostabilizing properties of mannosylglycerate and other compatible solutes on model enzymes. Extremophiles 6:209–216

    Article  CAS  Google Scholar 

  • Bünger J, Driller H (2004) Ectoine: an effective natural substance to prevent UVA-induced premature photoaging. Skin Pharmacol Physiol 17:232–237

    Article  Google Scholar 

  • Costa-Böddeker S, Thuyen LX, Schwarz A, Huy HD (2017) Diatom assemblages in surface sediments along nutrient and salinity gradients of Thi Vai estuary and Can Gio mangrove forest, Southern Vietnam. Estuar Coast 40:479–492

    Article  Google Scholar 

  • Detkova EN, Boltyanskaya YV (2007) Osmoadaptation of haloalkaliphilic bacteria: role of osmoregulators and their possible practical application. Microbiology 76:511–522

    Article  CAS  Google Scholar 

  • García MT, Mellado E, Ostos JC, Ventosa A (2004) Halomonas organivorans sp. nov., a moderate halophile able to degrade aromatic compounds. Int J Syst Evol Microbiol 54:1723–1728

    Article  Google Scholar 

  • Gasperotti AF, Revuelta MV, Studdert CA, Seitz MKH (2018) Identification of two different chemosensory pathways in representatives of the genus Halomonas. BMC Genomics 19:266

    Article  Google Scholar 

  • Guzmán H, Van-Thuoc D, Martín J, Hatti-Kaul R (2009) A process for the production of ectoine and poly(3-hydroxybutyrate) by Halomonas boliviensis. Appl Microbiol Biotechnol 84:1069–1077

    Article  Google Scholar 

  • Holguin G, Vazquez P, Bashan Y (2001) The role of sediment microorganisms in the productivity, conservation, and rehabilitation of mangrove ecosystems: an overview. Biol Fertil Soils 33:265–278

    Article  CAS  Google Scholar 

  • Kanapathipillai M, Lentzen G, Sierks M, Park CB (2004) Ectoine and hydroxyectoine inhibit aggregation and neurotoxicity of Alzheimer’s β-amyloid. FEBS Lett 579:4775–4780

    Article  Google Scholar 

  • Kunte HJ, Galinski EA, Trüper HG (1993) A modified FMOC-method for the detection of aminoacid type osmolytes and tetrahydropyrimidines (ectoines). J Microbiol Methods 17:129–136

    Article  CAS  Google Scholar 

  • Kunte HJ, Lentzen G, Galinski EA (2014) Industrial production of the cell protectant ectoine: production mechanisms, processes, and products. Curr Biotechnol 3:10–25

    Article  CAS  Google Scholar 

  • Lentzen G, Schwarz T (2006) Extremolytes: natural compounds from extremophiles for versatile applications. Appl Microbiol Biotechnol 72:623–634

    Article  CAS  Google Scholar 

  • Lippert G, Galinski EA (1992) Enzyme stabilization by ectoine-type compatible solutes: protection against heating, freezing and drying. Appl Microbiol Biotechnol 37:61–65

    Article  CAS  Google Scholar 

  • Malin G, Lapidot A (1996) Induction of synthesis of tetrahydropyrimidine derivatives in Streptomyces strains and their effect on Escherichia coli in response to osmotic and heat stress. J Bacteriol 178:385–395

    Article  CAS  Google Scholar 

  • Margesin R, Schinner F (2001) Potential of halotolerant and halophilic microorganisms for biotechnology. Extremophiles 5:73–83

    Article  CAS  Google Scholar 

  • Mata JA, Martínez-Cánovas J, Quesada E, Béjar V (2002) A detailed phenotypic characterization of the type strains of Halomonas species. Syst Appl Microbiol 25:360–375

    Article  CAS  Google Scholar 

  • Moh TH, Lau N-S, Furusawa G, Amirul A-AA (2017) Complete genome sequence of Microbulbifer sp. CCB-MM1, a halophile isolated from Matang mangrove forest, Malaysia. Stand Genomic Sci 12:36

    Article  Google Scholar 

  • Nagata S, Wang Y, Oshima A, Zhang L, Miyake H, Sasaki H, Ishida A (2008) Efficient cyclic system to yield ectoine using Brevibacterium sp. JCM 6894 subjected to osmotic downshock. Biotechnol Bioeng 99:941–948

    Article  CAS  Google Scholar 

  • Onraedt AE, Walcarius BA, Soetaert WK, Vandamme EJ (2005) Optimization of ectoine synthesis through fed-batch fermentation of Brevibacterium epidermis. Biotechnol Prog 21:1206–1212

    Article  CAS  Google Scholar 

  • Öztürk HU, Sariyar Akbulut B, Ayan B, Poli A (2015) Moderately halophilic bacterium Halomonas sp. AAD12: a promising candidate as a hydroxyectoine producer. J Microb Biochem Technol 7:262–268

    Google Scholar 

  • Pastor JM, Salvador M, Argandoña M, Bernal V (2010) Ectoines in cell stress protection: uses and biotechnological production. Biotechnol Adv 28:782–801

    Article  CAS  Google Scholar 

  • Roberts MF (2005) Organic compatible solutes of halotolerant and halophilic microorganism. Saline Syst (On-line Journal) 1:5

    Article  Google Scholar 

  • Sam K-K, Lau N-S, Furusawa G, Amirul A-AA (2018) Draft genome sequence of halophilic Hahella sp. strain CCB-MM4, isolated from Matang mangrove forests in Perak, Malaysia. Genome Announc 5:e01147–e01117

    Google Scholar 

  • Sauer T, Galinski EA (1998) Bacterial milking: a novel bioprocess for production of compatible solutes. Biotechnol Bioeng 57:306–313

    Article  CAS  Google Scholar 

  • Schröter MA, Meyer S, Hahn MB, Solomun T, Sturm H, Kunte HJ (2017) Ectoine protects DNA from damage by ionizing radiation. Sci Rep 7:15272

    Article  Google Scholar 

  • Ser H-L, Tan W-S, Mutalib N-SA, Yin W-F (2018) Genome sequence of Streptomyces mangrovisoli MUSC 149T isolated from intertidal sediments. Braz J Microbiol 49:13–15

    Article  CAS  Google Scholar 

  • Tamura K, Stecher G, Peterson D, Filipski A (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Bio Evol 30:2725–2729

    Article  CAS  Google Scholar 

  • Tuan VQ, Kuenzer C (2012) Can Gio mangrove biosphere reserve evaluation 2012: current status, dynamics and ecosystem services, IUCN, Hanoi, Vietnam 102 pp

  • Van-Thuoc D, Guzmán H, Quillaguamán J, Hatti-Kaul R (2010) High productivity of ectoines by Halomonas boliviensis using a combined two-step fed-batch culture and milking process. J Biotechnol 147:46–51

    Article  CAS  Google Scholar 

  • Van-Thuoc D, Huu-Phong T, Thi-Binh N, Thi-Tho N, Minh-Lam D, Quillaguamán J (2012) Polyester production by halophilic and halotolerant bacterial strains obtained from mangrove soil samples located in northern Vietnam. MicrobiologyOpen 1:395–406

    Article  CAS  Google Scholar 

  • Van-Thuoc D, Hashim SO, Hatti-Kaul R, Mamo G (2013) Ectoine mediated protection of enzyme from the effect of pH and temperature stress: a study using Bacillus halodurans xylanase as a model. Appl Microbiol Biotechnol 97:6271–6278

    Article  CAS  Google Scholar 

  • Werkhäuser N, Bilstein A, Sonnemann U (2014) Treatment of allergic rhinitis with ectoine containing nasal spray and eye drops in comparison with azelastine containing nasal spray and eye drops or with cromoglycic acid containing nasal spray. J Allergy:176597

  • Zhang LH, Lang YJ, Nagata S (2009) Efficient production of ectoine using ectoine-excreting strain. Extremophiles 13:717–724

    Article  CAS  Google Scholar 

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Funding

This research was supported by the Ministry of Education and Training (Grant B2007-SHP-32) and Vietnam National Foundation for Science and Technology Development (Nafosted) (Grant 106-NN.04-2016.11).

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Authors and Affiliations

  1. Department of Biotechnology and Microbiology, Faculty of Biology, Hanoi National University of Education, 136 Xuan Thuy, Cau Giay, Hanoi, Vietnam

    Doan Van Thuoc & Tran Thi Hien

  2. School of Biological Sciences, Universiti Sains Malaysia, 11800, Penang, Malaysia

    Kumar Sudesh

Authors
  1. Doan Van Thuoc
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  2. Tran Thi Hien
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  3. Kumar Sudesh
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Correspondence to Doan Van Thuoc.

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Van Thuoc, D., Hien, T.T. & Sudesh, K. Identification and characterization of ectoine-producing bacteria isolated from Can Gio mangrove soil in Vietnam. Ann Microbiol 69, 819–828 (2019). https://doi.org/10.1007/s13213-019-01474-7

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