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Annals of Microbiology

, Volume 68, Issue 5, pp 287–294 | Cite as

The response of LuxS/AI-2 quorum sensing in Lactobacillus fermentum 2-1 to changes in environmental growth conditions

  • Yue Gu
  • Bo Li
  • Jianjun Tian
  • Rong Wu
  • Yinfeng He
Original Article
  • 186 Downloads

Abstract

Quorum sensing (QS) is a communication mechanism based on the production of autoinducers in order to regulate cooperative behavior and physiological traits among bacteria. Autoinducer-2 (AI-2) is mediated by luxS and pfs genes and is used for both intra- and interspecies communication. The effects of environmental stresses, such as pH, temperature, osmotic, and nutrient stresses, were investigated on the LuxS/AI-2 QS system in Lactobacillus fermentum 2-1. The AI-2 activity in L. fermentum 2-1 was induced by stress responses to acid shock, high temperature, and nutrient inadequacy while a decrease in activity was correlated with high pH, low temperature, and osmotic stress. Maximum transcription of luxS and pfs was achieved after L. fermentum 2-1 was exposed to acid shock, low temperature, high concentration of NaCl, and diluted MRS broth. The results indicate that various stresses affected the LuxS/AI-2 QS system, which may be involved in the resistance process of the L. fermentum 2-1.

Keywords

Quorum sensing Lactobacillus fermentum 2-1 Autoinducer-2 Transcription Environmental stresses 

Notes

Funding information

This project was supported by the Program of National Natural Science Foundation of China (No. 31360396).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature and the University of Milan 2018

Authors and Affiliations

  • Yue Gu
    • 1
  • Bo Li
    • 1
  • Jianjun Tian
    • 1
  • Rong Wu
    • 1
  • Yinfeng He
    • 1
  1. 1.College of Food Science and EngineeringInner Mongolia Agricultural UniversityHohhotChina

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