Annals of Microbiology

, Volume 68, Issue 5, pp 273–286 | Cite as

Bacterial community structure associated with the rhizosphere soils and roots of Stellera chamaejasme L. along a Tibetan elevation gradient

  • Hui Jin
  • Xiaoyan Yang
  • Rentao Liu
  • Zhiqiang Yan
  • Xudong Li
  • Xiuzhuang Li
  • Anxiang Su
  • Yuhui Zhao
  • Bo Qin
Original Article


The effect of altitude on the composition and diversity of microbial communities have attracted highly attention recently but is still poorly understood. We used 16S rRNA gene clone library analyses to characterize the bacterial communities from the rhizosphere and roots of Stellera chamaejasme in the Tibetan Plateau. Our results revealed that Actinobacteria and Proteobacteria were dominant bacteria in this medicinal plant in the rhizosphere and root communities. The Shannon diversity index showed that the bacterial diversity of rhizosphere follows a small saddle pattern, while the roots possesses of a hump-backed trend. Significant differences in the composition of bacterial communities between rhizosphere and roots were detected based on multiple comparisons analysis. The community of Actinobacteria was found to be significantly negative correlated with soil available P (p < 0.01), while the phylum of Proteobacteria showed a positive relationship with available P (p < 0.05). Moreover, redundancy analysis indicated that soil phosphorus, pH, latitude, elevation and potassium positively correlated with bacterial communities associated with rhizosphere soils. Taken together, we provide evidence that bacterial communities associated with S. chamaejasme exhibited some certain elevational pattern, and bacterial communities of rhizosphere soil were regulated by environmental characteristics along elevational gradients in this alpine ecosystem.


Bacterial community Phylogenetic diversity Stellera chamaejasme L. Tibetan Plateau Elevation gradient 



The authors are grateful the Professor Frank Stermitz for assistance with language editing. This work was financially supported by the National Key Research and Development Program (2017YFD0200804), the National Natural Science Foundation of China (No. 31772668, 21775154, 31560037 and 31570354), the Open Project Program of Breeding Base for State Key Laboratory of Land Degradation and Ecological Restoration of North-western China/Key Laboratory for Restoration and Reconstruction of Degraded Ecosystem in North-western China of Ministry of Education (2017KF009), the Open Project Program for State Key Laboratory of Grassland Agro-ecosystem of Lanzhou University (SKLGAE201704), Basic Research Program of Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (080423SYR1), the Youth Science Foundations of Gansu Province (1506RJYA294), and Around five top priorities program of “One-Three-Five” Strategic Planning of Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

13213_2018_1336_MOESM1_ESM.pdf (1.9 mb)
ESM 1 (PDF 1971 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature and the University of Milan 2018

Authors and Affiliations

  • Hui Jin
    • 1
    • 2
  • Xiaoyan Yang
    • 2
  • Rentao Liu
    • 1
  • Zhiqiang Yan
    • 2
  • Xudong Li
    • 3
  • Xiuzhuang Li
    • 2
  • Anxiang Su
    • 4
  • Yuhui Zhao
    • 5
  • Bo Qin
    • 2
  1. 1.State Key Laboratory of Land Degradation and Ecological Restoration of North-western China and Key Laboratory for Restoration and Reconstruction of Degraded Ecosystem in North-western China of Ministry of EducationYinchuanPeople’s Republic of China
  2. 2.CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical PhysicsChinese Academy of Sciences (CAS)LanzhouPeople’s Republic of China
  3. 3.State Key Laboratory of Grassland Agro-ecosystem, College of Pastoral Agriculture Science and TechnologyLanzhou UniversityLanzhouPeople’s Republic of China
  4. 4.Institute for the Control of AgrochemicalsMinistry of Agriculture (ICAMA)BeijingPeople’s Republic of China
  5. 5.Institute of BiologyGansu Academy of SciencesLanzhouPeople’s Republic of China

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