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Bacterial community structure associated with the rhizosphere soils and roots of Stellera chamaejasme L. along a Tibetan elevation gradient

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Abstract

The effect of altitude on the composition and diversity of microbial communities have attracted highly attention recently but is still poorly understood. We used 16S rRNA gene clone library analyses to characterize the bacterial communities from the rhizosphere and roots of Stellera chamaejasme in the Tibetan Plateau. Our results revealed that Actinobacteria and Proteobacteria were dominant bacteria in this medicinal plant in the rhizosphere and root communities. The Shannon diversity index showed that the bacterial diversity of rhizosphere follows a small saddle pattern, while the roots possesses of a hump-backed trend. Significant differences in the composition of bacterial communities between rhizosphere and roots were detected based on multiple comparisons analysis. The community of Actinobacteria was found to be significantly negative correlated with soil available P (p < 0.01), while the phylum of Proteobacteria showed a positive relationship with available P (p < 0.05). Moreover, redundancy analysis indicated that soil phosphorus, pH, latitude, elevation and potassium positively correlated with bacterial communities associated with rhizosphere soils. Taken together, we provide evidence that bacterial communities associated with S. chamaejasme exhibited some certain elevational pattern, and bacterial communities of rhizosphere soil were regulated by environmental characteristics along elevational gradients in this alpine ecosystem.

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Acknowledgements

The authors are grateful the Professor Frank Stermitz for assistance with language editing. This work was financially supported by the National Key Research and Development Program (2017YFD0200804), the National Natural Science Foundation of China (No. 31772668, 21775154, 31560037 and 31570354), the Open Project Program of Breeding Base for State Key Laboratory of Land Degradation and Ecological Restoration of North-western China/Key Laboratory for Restoration and Reconstruction of Degraded Ecosystem in North-western China of Ministry of Education (2017KF009), the Open Project Program for State Key Laboratory of Grassland Agro-ecosystem of Lanzhou University (SKLGAE201704), Basic Research Program of Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (080423SYR1), the Youth Science Foundations of Gansu Province (1506RJYA294), and Around five top priorities program of “One-Three-Five” Strategic Planning of Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences.

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Authors and Affiliations

  1. State Key Laboratory of Land Degradation and Ecological Restoration of North-western China and Key Laboratory for Restoration and Reconstruction of Degraded Ecosystem in North-western China of Ministry of Education, Yinchuan, 750021, People’s Republic of China

    Hui Jin & Rentao Liu

  2. CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, 730000, People’s Republic of China

    Hui Jin, Xiaoyan Yang, Zhiqiang Yan, Xiuzhuang Li & Bo Qin

  3. State Key Laboratory of Grassland Agro-ecosystem, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730000, People’s Republic of China

    Xudong Li

  4. Institute for the Control of Agrochemicals, Ministry of Agriculture (ICAMA), Beijing, 100125, People’s Republic of China

    Anxiang Su

  5. Institute of Biology, Gansu Academy of Sciences, Lanzhou, 730000, People’s Republic of China

    Yuhui Zhao

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  1. Hui Jin
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  9. Bo Qin
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Jin, H., Yang, X., Liu, R. et al. Bacterial community structure associated with the rhizosphere soils and roots of Stellera chamaejasme L. along a Tibetan elevation gradient. Ann Microbiol 68, 273–286 (2018). https://doi.org/10.1007/s13213-018-1336-0

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