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Characterization of bacteriocin from Lactococcus isolated from traditional Algerian dairy products

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Abstract

Mesophilic strains of lactic acid bacteria belonging to the genus Lactococcus were isolated from traditional dairy products and selected for their ability to produce antimicrobial substances such as bacteriocins. Six strains: Lactococcus lactis ssp. lactis (strains Lc l1, Lc l3, Lc l5), Lactococcus lactis ssp. cremoris (strains Lc c4, Lc c6) and Lactococcus lactis ssp. lactis biovar diacetylactis (strain Lc d2) were selected for their antagonistic activities against different groups of microorganisms. The most active strain, Lactococcus lactis ssp. lactis (Lc l5), produced its inhibitory substance (Bac5) in M17 broth presenting an important inhibition mainly against Listeria monocytogenes. It had no effect on its producer strain. These properties suggested that the inhibitory substance could be considered as a bacteriocin-like substance. SDS-PAGE analyses indicated that it was a homogeneous protein of higher molecular weight of 67 kDa. The bacteriocin was stable over a wide range of pH and temperature; it was active after autoclaving and was not affected by storage at −20°C for 6 months. Moreover, it was sensitive to proteolytic enzymes confirming the proteinaceous nature of the inhibitory substance.

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References

  • Ananou S, Baños A, Maqueda M, Martínez-Bueno M, Gálvez A, Valdivia E (2010) Effect of combined physico-chemical treatments based on enterocin AS-48 on the control of Listeria monocytogenes and Staphylococcus aureus in a model cooked ham. Food Control 21:478–486

    Article  CAS  Google Scholar 

  • Anastasiadou S, Papagianni M, Ambrosiadis I, Koidis P (2008) Rapid quantifiable assessment of nutritional parameters influencing pediocin production by Pediococcus acidilactici NRRL B5627. Bioresource Technol 99:6646–6650

    Article  CAS  Google Scholar 

  • Aslim B, Yuksekdag ZN, Sarikaya E, Beyatli Y (2005) Determination of the bacteriocin-like substances produced by some lactic acid bacteria isolated from Turkish dairy products. LWT 38:691–694

    Article  CAS  Google Scholar 

  • Ben Omar N, Ampe F, Raimbault M, Guyot JP, Tailliez P (2000) Molecular diversity of lactic acid bacteria from cassava sour starch Colombia. Syst Appl Microbiol 23:285–291

    Article  Google Scholar 

  • Bhunia AK, Johnson MC, Ray B (1987) Direct detection of an antimicrobial peptide of Pediococcus acidilactici in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. J Ind Microbiol 2:319–322

    Article  CAS  Google Scholar 

  • Dalié DKD, Deschamps AM, Richard-Forget F (2010) Lactic acid bacteria – Potential for control of mould growth and mycotoxins: A review. Food Control 21:370–380

    Article  Google Scholar 

  • Davey GP, Richardson BC (1981) Purification and some properties of diplococcin from Streptococcus cremoris 346. Appl Environ Microbiol 41:84–89

    PubMed  CAS  Google Scholar 

  • De Kwaadsteniet M, Todorov SD, Knoetze H, Dicks LMT (2005) Characterization of a 3944 Da bacteriocin, produced by Enterococcus mundtii ST15, with activity against Gram-positive and Gram-negative bacteria. Int J Food Microbiol 105:433–444

    Article  PubMed  Google Scholar 

  • De Vuyst L, Leroy F (2007) Bacteriocins from Lactic Acid Bacteria: Production purification and Food Applications. J Mol Microb Biotech 13:194–199

    Article  Google Scholar 

  • Delgado A, Brito D, Fevererio P, Peres C, Marques JF (2001) Antimicrobial activity of Lactobacillus plantarum isolated from a traditional lactic acid fermentation of table olives. Lait 8:203–215

    Article  Google Scholar 

  • Ghalfi H, Benkerroum N, Ongena M, Bensaid M, Thonart P (2010) Production of three anti-listerial peptides by Lactobacillus curvatus in MRS broth. Food Res Inter 43:33–39

    Article  CAS  Google Scholar 

  • Gies A, Singh J, Teuber M (1983) Potential of lactic streptococci to produce bacteriocin. Appl Environ Microbiol 45:205–211

    Google Scholar 

  • Gobetti M, Corsetti A, Smacchi E, Rossi J (1997) Purification and characterization of proteinaceous compound from Pseudomonas fluorescens ATCC 948 with inhibitory activity against some gram-positive and gram negative bacteria of interest. Lait 77:267–278

    Article  Google Scholar 

  • Gomez S, Cosson C, Deschamps AM (1997) Evidence for a bacteriocin – like substance produced by a new strain of Streptococcus sp, inhibitory to Gram positive food – borne pathogens. Res Microbiol 148:757–766

    Article  PubMed  CAS  Google Scholar 

  • Guerrieri E, Niederhäusern S, Messi P, Sabia C, Iseppi R, Anacarso I, Bondi M (2009) Use of lactic acid bacteria (LAB) biofilms for the control of Listeria monocytogenes in a small-scale model. Food Control 20:861–865

    Article  CAS  Google Scholar 

  • Henning S, Metz R, Hammes WP (1986) Studies on the mode of action of nisin. Int J Food Microbiol 3:121–134

    Article  CAS  Google Scholar 

  • Holo H, Jeknic Z, Daeschel M, Stevanovic S, Nes IF (2001) Plantaricin W from Lactobacillus plantarum belongs to a new family of two-peptide lantibiotics. Microbiology 147:643–651

    PubMed  CAS  Google Scholar 

  • Jack RW, Tagg RJ, Ray B (1995) Bacteriocins of Gram positive bacteria. Microbiol Rev 59:171–200

    PubMed  CAS  Google Scholar 

  • Klaenhammer TR (1988) Bacteriocins of lactic acid bacteria. Biochimie 70:337–349

    Article  PubMed  CAS  Google Scholar 

  • Lee NK, Paik HD (2001) Partial characterization of lactin NK 24 a newly identified bacteriocin of Lactococcus lactis NK 24 molted from Jeat gal. Food Microbiol 18:17–24

    Article  CAS  Google Scholar 

  • Lewus CB, Kaiser A, Montville TJ (1991) Inhibition of food- borne bacterial pathogens by bacteriocins from lactic acid bacteria solated from meat. Appl Environ Microb 57:1683–1688

    CAS  Google Scholar 

  • Lima FL, Farias FF, Costa JE, Auxiadora MR, Alviano CS, Farias LM (2002) Bacteriocin production by Actinobacillus actinomy-cetemcomitans isolated from oral cavity of humans with periodontal diereses periodontal heathy subjects and marmosets. Res Microbiol 153:45–52

    Article  CAS  Google Scholar 

  • Liu W, Hansen N (1990) Some chemical and physiological properties of nisin a small-protein antibiotic produced by Lactococcus lactis. Appl Environ Microbiol 56:2551–2558

    PubMed  CAS  Google Scholar 

  • Maldonado-Barragán A, Caballero-Guerrero B, Jiménez E, Jiménez-Díaz R, Ruiz-Barba JL, Rodríguez JM (2009) Enterocin C a class IIb bacteriocin produced by E faecalis C901 a strain isolated from human colostrums. Int J Food Microbiol 133:105–112

    Article  PubMed  Google Scholar 

  • Mao Y, Muriana PM, Cousin MA (2001) Purification and transpositional inactivation of Lacticin FS92 a broad-spectrum bacteriocin produced by Lactococcus lactis FS92. Food Microbiol 18:165–175

    Article  CAS  Google Scholar 

  • Martinez RCR, De Martinis ECP (2005) Antilisterial activity of a crude preparation of Lactobacillus sakei 1 bacteriocin and its lack of influence on Listeria monocytogenes haemolytic activity. Food Control 16:429–433

    Article  Google Scholar 

  • Molinos AC, Abriouel H, Lucas López R, Ben Omar N, Valdivia E, Gálvez A (2009) Enhanced bactericidal activity of enterocin AS-48 in combination with essential oils natural bioactive compounds and chemical preservatives against Listeria monocytogenes in ready-to-eat salad. Food Chem Toxicol 47:2216–2223

    Article  Google Scholar 

  • Moll G, Ubbink-Kok T, Hildeng-Hauge H, Nissen-Meyer J, Nes I, Konings WN, Drissen AJM (1996) Lactococcin G Is a Potassium ion-conduting two component bacteriocin. J Bacteriol 178:600–605

    PubMed  CAS  Google Scholar 

  • Muñoz A, Maqueda M, Gálvez A, Martínez-Bueno M, Rodríguez A, Valdivia E (2004) Biocontrol of Psychrotrophic Enterotoxigenic Bacillus cereus in a Nonfat Hard Cheese by an Enterococcal Strain–Producing Enterocin AS-48. J Food Protect 67:1517–1521

    Google Scholar 

  • Oelschlaeger TA (2010) Mechanisms of probiotic actions – A review. Int J Med Microbiol 3001:57–62

    Article  Google Scholar 

  • Piard JC, Desmazeaud M (1992) Inhibiting factors produced by lactic acid bacteria 2- Bacteriocins and other antibacterial substances. Lait 72:113–142

    Article  CAS  Google Scholar 

  • Piva A, Headon RD (1994) Pediocin A a bacteriocin produced by Pediococcus pentosaceus FBB61. Microbiology 140:697–702

    Article  PubMed  CAS  Google Scholar 

  • Rbsslanda E, Langsruda T, Granumb PE, Sbrhauga T (2005) Production of antimicrobial metabolites by strains of Lactobacillus or Lactococcus co-cultured with Bacillus cereus in milk. Int J Food Microbiol 98:193–200

    Article  Google Scholar 

  • Rivera-Espinoza Y, Gallardo-Navarro Y (2010) Non-dairy probiotic products. Food Microbiol 27:1–11

    Article  PubMed  Google Scholar 

  • Rodriguez JM, Martinez MI, Kok J (2002) Pediocin PA-1 a wide spectrum bacteriocin from lactic acid bacteria. Crit Rev Food Sci Nut 42:91–121

    Article  CAS  Google Scholar 

  • Rogers LA, Whittier ED (1928) Limiting factors in lactic fermentation. J Bacteriol 16:211–229

    PubMed  CAS  Google Scholar 

  • Ryan MP, Rea MC, Hill C, Ross PR (1996) In application in cheddar cheese manufacture flora strain of Lactococcus lactis producing a novel broad spectrum bacteriocin Lacticin 3147. Appl Environ Microbiol 62:612–619

    PubMed  CAS  Google Scholar 

  • Schillinger U, Lücke FK (1987) Identification of lactobacilli from meat and meat products. Food Microbiol 4:199–208

    Article  Google Scholar 

  • Schöbitz R, Zaror R, Leon TO, Costa M (1999) A bacteriocin from Carnobacterium pisicola for the control of Listeria monocytogenes in vacuum packaged meat. Food Microbiol 16:249–255

    Article  Google Scholar 

  • Schved F, Henis Y, Juven BJ (1994) Response of spheroplasts and chelator-permeabilized cells of Gram-negative bacteria to the action of the bacteriocins pediocin SJ-1 and nisin. Int J Food Microbiol 21:305–314

    Article  PubMed  CAS  Google Scholar 

  • Tagg JR, Dajani AS, Wannamaker LW (1976) Bacteriocins of Gram positive bacteria. Bacteriol Rev 40:722–756

    PubMed  CAS  Google Scholar 

  • Terzaghi BE, Sandine WE (1975) Improved medium for lactic streptococci and their bacteriophages. Appl Microbiol 29:807–813

    PubMed  CAS  Google Scholar 

  • Thompson JK, Collins MA, Merler WD (1996) Characterization of proteinaceous antimicrobial produced by Lactobacillus helveticus CNRZ 450. J Appl Bacteriol 80:338–348

    Article  PubMed  CAS  Google Scholar 

  • Thuault D, Belliard E, Le Guern J, Bourgeois CM (1991) Inhibition of Clostridium tyrobutyricum by bacteriocin like substances produced by lactic acid bacteria. J Dairy Sci 74:1145–1150

    Article  PubMed  CAS  Google Scholar 

  • Yildrim Z, Johnson MG (1998) Detection and characterization of a bacteriocin produced by Lactococcus lactis ssp. cremoris R isolated from radish. Lett Appl Microbiol 26:297–304

    Article  Google Scholar 

Download references

Acknowledgement

The authors gratefully acknowledge the Pasteur Institute of Algiers for providing the target strains used in this study.

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Authors and Affiliations

  1. Laboratory of Science and Environmental Techniques, National Polytechnic School, BP 182,, Hassen Badi 16200 El Harrach, Algiers, Algeria

    Amina Hellal & Linda Amrouche

  2. National Agronomic Institute, Hassen Badi, 16200 El Harrach, Algiers, Algeria

    Zoulikha Ferhat

  3. University of Science and Technology Houari Boumediène, BP 32, El Alia 16111 Bab-Ezzouar, Algiers, Algeria

    Fatima Laraba

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  1. Amina Hellal
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  2. Linda Amrouche
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  3. Zoulikha Ferhat
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  4. Fatima Laraba
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Correspondence to Amina Hellal.

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Hellal, A., Amrouche, L., Ferhat, Z. et al. Characterization of bacteriocin from Lactococcus isolated from traditional Algerian dairy products. Ann Microbiol 62, 177–185 (2012). https://doi.org/10.1007/s13213-011-0244-3

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  • DOI: https://doi.org/10.1007/s13213-011-0244-3

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