Abstract
Konjac glucomannan oligosaccharide has attracted much attention due to its broad biological activities. Specific glucomannan degrading enzymes are effective tools for the production of oligosaccharides from konjac glucomannan. However, there are still few reports of commercial enzymes that can specifically degrade konjac glucomannan. The gene ppgluB encoding a glucomannanase consisting of 553 amino acids (61.5 kDa) from Paenibacillus polymyxa 3–3 was cloned and heterologous expressed in Escherichia coli BL21 (DE3). The recombinant PpGluB showed high specificity for the degradation of konjac glucomannan. Moreover, the hydrolytic products of PpGluB degrade konjac glucomannan were a series of oligosaccharides with degrees of polymerisation of 2–12. Furthermore, the biochemical properties indicated that PpGluB is the optimal active at 45 to 55 °C and pH 5.0–6.0, and shows highly pH stability over a very broad pH range. The present characteristics indicated that PpGluB is a potential tool to be used to produce oligosaccharides from konjac glucomannan.
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Acknowledgements
This work was supported by Key Laboratory of Se-enriched Products Development and Quality Control, Ministry of Agriculture and Rural Affairs/National Local Joint Engineering Laboratory of Se enriched Food Development (Se-2018A02, Se-2020C02); China Se-enriched Industry Research Institute Se-enriched Special 236 Plan Project (2019QCY-2.2); National Key R&D Program of China (2017YFD0200900) Subject 2 (2017YFD0200902). Dr. Heng Yin was supported by Liaoning Revitalization Talents Program, China (XLYC1807041).
Funding
This work was supported by Key Laboratory of Se-enriched Products Development and Quality Control, Ministry of Agriculture and Rural Affairs/National Local Joint Engineering Laboratory of Se enriched Food Development (Se-2018A02, Se-2020C02); China Se-enriched Industry Research Institute Se-enriched Special 236 Plan Project (2019QCY-2.2); National Key R&D Program of China (2017YFD0200900) Subject 2 (2017YFD0200902). Dr. Heng Yin was suppoted by Liaoning Revitalization Talents Program, China (XLYC1807041).
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All authors contributed to the study conception and design. HY and JL designed the outline of the article. The first draft of the manuscript was written by KL and all authors commented on previous versions of the manuscript. CJ, HT and JL searched the literature and related information. QL, XZ, DT and YX provided scientific feedback and critical comments to revise the content. All authors read and approved the final manuscript.
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Li, K., Jiang, C., Tan, H. et al. Identification and characterization of a novel glucomannanase from Paenibacillus polymyxa. 3 Biotech 11, 129 (2021). https://doi.org/10.1007/s13205-021-02676-0
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DOI: https://doi.org/10.1007/s13205-021-02676-0