Lignin degradation and nutrient cycling by white rot fungi under the influence of pesticides

Abstract

The production of enzymes involved in lignin degradation (laccase, ligninase), carbon cycling (β-glucosidase), and phosphorous cycling (phosphomonoesterase) by white rot fungi (Pleurotus sajor-caju) was studied. In the presence of chlorpyrifos, carbofuran, and their mixture, laccase activity was highest on the 7th day, i.e., 192.5 ± 0.31 U ml− 1, 213.6 ± 0.31 U ml− 1, and 164.6 ± 0.31 U ml− 1, respectively, compared to the control which produced maximum laccase on the 14th day (126.9 ± 0.15 U ml− 1). Phosphomonoesterase activity in the presence of chlorpyrifos, carbofuran, and their mixture was 31.5 ± 0.25, 24.1 ± 0.15, and 29.2 ± 0.35 µg PNP min−1 ml−1, respectively, which was more than the control on the 21st day (11.63 ± 0.21 µg PNP min−1 ml−1). β-Glucosidase production increased with the days of incubation in the presence of pesticides than in the control. β-Glucosidase activity on the 21st day in the presence of chlorpyrifos, carbofuran, and their mixture was 32.4 ± 0.1, 24.2 ± 0.3, and 28.4 ± 0.25 µg PNP min−1 ml−1, respectively, as compared to control (15.3 ± 0.6 µg PNP min−1 ml−1). Thus, chlorpyrifos, carbofuran, and their mixture were found to have a positive effect on the production of laccase, β-glucosidase, and phosphomonoesterase by P. sajor-caju, which can use these pesticides as a source of their nutrition, thereby improving the health of pesticide-polluted soils.

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References

  1. Bhalerao TS, Puranik PR (2007) Biodegradation of organochlorine pesticide, endosulfan, by a fungal soil isolate, Aspergillus niger. Int Biodeterior Biodegrad 59:315–321

    CAS  Article  Google Scholar 

  2. Bozell JJ, Petersen GR (2010) Technology development for the production of bio based products from biorefinery carbohydrates—the US Department of Energy’s ‘Top 10’ revisited. Green Chem 12:539–554

    CAS  Article  Google Scholar 

  3. Brandt A, Chen L, van Dongen BE, Welton T, Hallett JP (2015) Structural changes in lignins isolated using an acidic ionic liquid water mixture. Green Chem 17:5019–5034

    CAS  Article  Google Scholar 

  4. Buswell J (1994) Potential of Spent Mushroom Substrate for Bioremediation Purposes. Compost Sci Util 2:31–36

    Article  Google Scholar 

  5. Canet R, Birnstingl J, Malcolm D, Lopez-Real J, Beck A (2001) Biodegradation of polycyclic aromatic hydrocarbons (PAHs) by native microflora and combinations of white-rot fungi in a coal-tar contaminated soil. Bioresource Tech 76:113–117

    CAS  Article  Google Scholar 

  6. Chatel G, Rogers RD (2013) Review: oxidation of lignin using ionic liquids—an innovative strategy to produce renewable chemicals. ACS Sustain Chem Eng 2:322–339

    Article  Google Scholar 

  7. El-Ghany A, Masmal IA (2016) Fungal biodegradation of organophosphorus insecticides and their impact on soil microbial population. J Plant Pathol Microbiol 7(5):349

    Google Scholar 

  8. Fragoeiro S (2005) Use of fungi in bioremediation of pesticides. Dissertation, Cranfield University, Bedford, pp 3–217 

  9. Keshri G, Magan N (2000) Detection and differentiation between mycotoxigenic and non-mycotoxigenic strains of two Fusarium spp. using volatile production profiles and hydrolytic enzymes. J Appl Microb 89(5):825–833

    CAS  Article  Google Scholar 

  10. Kumari BSS, Praveen K, Usha KY, Kumar KD, Reddy GPK, Reddy BR (2019) Ligninolytic behavior of the white rot fungus Stereum ostrea under influence of culture conditions, inducers and chlorpyrifos. 3 Biotech 9:424–435

    Article  Google Scholar 

  11. Nawaz K, Hussain K, Choudary N, Majeed A, Ilyas U, Ghani A, Lin F, Ali K, Afghan S, Raza G, Lashari MI (2011) Eco-friendly role of biodegradation against agricultural pesticides hazards. Afr J Microbiol Res 5(3):177–183

    Google Scholar 

  12. Sundman V, Nase L (1971) A simple plate test for direct visualization of biological lignin degradation. Pap Timber 53:67–71

    CAS  Google Scholar 

  13. Singleton I (2001) Fungal remediation of soils contaminated with persistent organic pollutants. In: Gadd G (ed) Fungi in bioremediation. Cambridge University Press, UK

    Google Scholar 

  14. Taylor J, Wilson B, Mills M, Burns R (2002) Comparison of microbial numbers and enzymatic activities in surface soils and subsoils using various techniques. Soil Biol Biochem 34:387–401

    CAS  Article  Google Scholar 

  15. Trejo-Hernandez M, Lopez-Munguia A, Ramirez R (2001) Residual compost of Agaricus bisporus as a source of crude laccase for enzymic oxidation of phenolic compounds. Process Biochem 36:635–639

    CAS  Article  Google Scholar 

Download references

Acknowledgements

The authors are highly thankful to the Division of Biochemistry, Faculty of Basic Sciences, SKUAST-J, for providing necessary facilities in carrying out the research work of Ph.D dissertation.

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Correspondence to Moni Gupta.

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Wali, A., Gupta, M., Gupta, S. et al. Lignin degradation and nutrient cycling by white rot fungi under the influence of pesticides. 3 Biotech 10, 266 (2020). https://doi.org/10.1007/s13205-020-02251-z

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Keywords

  • White rot fungi
  • Laccase
  • β-Glucosidase
  • Phosphomonoesterase
  • Chlorpyrifos
  • Carbofuran