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3 Biotech

, 8:261 | Cite as

Cyclic lipopeptide biosurfactant from Bacillus tequilensis exhibits multifarious activity

  • Arun Kumar Pradhan
  • Animesha Rath
  • Nilotpala Pradhan
  • Rupenangshu Kumar Hazra
  • Rati Ranjan Nayak
  • Sanjit Kanjilal
Original Article
  • 68 Downloads

Abstract

Bacillus tequilensis strain CH had been previously shown to produce a biosurfactant. In this study, chemical structure of the purified biosurfactant was determined by using high performance liquid chromatography and liquid chromatography-mass spectroscopy as a 10 amino acid cyclic lipopeptide (CL). The cyclic lipopeptide was found to be active against Anopheles culicifacies larvae with a LC50 of 110 µg/ml in 2 days. 1 ppm cadmium (Cd) which had a profound mutagenic effect on the cell division of onion (Allium cepa) root tip cell resulting in abnormal metaphase, abnormal anaphase and nuclei elongation was partially reversed in the presence of 0.1 mg/ml of CL (52% cells dividing normally and 8% with abnormal division) and was comparable to control experiment where no Cd was present. Thus, the CL described in this report may have applications in eliminating larvae from water repository systems and in reversing the effects of cadmium pollution.

Keywords

Biosurfactants Lipopeptide Larvicidal Bioremediation 

Notes

Acknowledgements

The authors intend to show a deep sense of gratitude to the Honourable President Dr. Manoj Nayak, Siksha ‘O’ Anusandhan, Deemed to be University for permitting to publish scientific literature. The authors also thank Regional Centre for Medical Research, and CSIR-IMMT, Odisha for providing scientific support.

Compliance with ethical standards

Conflict of interest

No conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Arun Kumar Pradhan
    • 1
    • 2
  • Animesha Rath
    • 3
  • Nilotpala Pradhan
    • 4
  • Rupenangshu Kumar Hazra
    • 3
  • Rati Ranjan Nayak
    • 5
  • Sanjit Kanjilal
    • 5
  1. 1.Centre for BiotechnologySiksha O Anusandhan (Deemed to be University)BhubaneswarIndia
  2. 2.Odisha NET Academy & Research CentreBhubaneswarIndia
  3. 3.Entomology DepartmentICMR-Regional Medical Research CentreBhubaneswarIndia
  4. 4.Environment and Sustainability DepartmentCSIR-Institute of Minerals and Materials TechnologyBhubaneswarIndia
  5. 5.Lipid and TechnologyCSIR-Indian Institute of Chemical TechnologyHyderabadIndia

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