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Increasing of activity and thermostability of cold active butanol-tolerant endoglucanase from a marine Rhodococcus sp. under high concentrations of butanol condition

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A Correction to this article was published on 19 July 2018

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Abstract

Optimum pH and temperature of the endoglucanase from Rhodococcus sp. was 5.0 and 40 °C. The endoglcuanase activity was 1.18 folds higher in 5% butanol solution than that in butanol-free solution. Melt point temperature was 2.5 °C higher in 5% butanol solution (50.0 °C) than that in butanol-free solution (47.5 °C). At 45–60 °C, Gbbs-free energy change, ∆G, was higher in 5% butanol solution than that in butanol-free solution. The content of α-helix and β-sheet increased in 5% butanol solution. The increasing of α-helix and β-sheet content led to higher activity and better thermostability in butanol solution. The cold active butanol-tolerant endoglucanase was valuable for biobutanol production by a simultaneous saccharification and fermentation process.

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Change history

  • 19 July 2018

    The original article can be found online.

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Acknowledgements

This work was supported by national natural science foundation of China (31271928) and Hubei Provincial Department of Education (Project: Design of a butanol-tolerant endoglucanase and butanol-tolerant mechanism, 2017B041).

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Authors and Affiliations

  1. Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Key Laboratory of Industrial Microbiology, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei University of Technology, Wuhan, 430068, People’s Republic of China

    Xuhao Zeng & Dongsheng Xue

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  1. Xuhao Zeng
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  2. Dongsheng Xue
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Correspondence to Dongsheng Xue.

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Zeng, X., Xue, D. Increasing of activity and thermostability of cold active butanol-tolerant endoglucanase from a marine Rhodococcus sp. under high concentrations of butanol condition. 3 Biotech 8, 265 (2018). https://doi.org/10.1007/s13205-018-1249-4

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