3 Biotech

, 8:212 | Cite as

Renoprotective effect of cinnamaldehyde in food color induced toxicity

  • Uma Kant Sharma
  • Ramesh Kumar
  • Ashutosh Gupta
  • Risha Ganguly
  • Abhay K. Pandey
Short Reports


Present study reports the effects of metanil yellow, a non-permitted food colouring dye, on the biomarkers of oxidative stress and kidney function in blood and renal tissue of albino Wistar rats and its mitigation by cinnamaldehyde, a major phytoconstituents of cinnamon. Oral administration of metanil yellow in rats caused about 70% reduction in ferric reducing ability (FRAP 5.1 μM/L) and 50% decline in reduced glutathione (GSH 59.27 nM/mg protein) content in plasma with simultaneous increase in serum creatinine level. In kidney tissues, activities of superoxide dismutase (SOD), catalase and GSH dropped while malondialdehyde (MDA) content increased. Co-administration of cinnamaldehyde with metanil yellow showed considerable restorative effect on the biomarkers of plasma antioxidant status and kidney function i.e., FRAP (11.5 μM/L), GSH (83–88.5 nM/mg protein), urea, creatinine, SOD, catalase and MDA. Administration of cinnamaldehyde restored the kidney enzyme activities up to 75% of the base level. The study revealed that reno-protective action of cinnamaldehyde was mediated by lowering oxidative stress level.


Cinnamaldehyde Metanil yellow Oxidative stress Renoprotective Food colour 



UKS, RK, AG and RG acknowledge financial support in the form of UGC CRET fellowships. All authors also acknowledge UGC-SAP and DST-FIST Facilities of the Department of Biochemistry, University of Allahabad, Allahabad.

Compliance with ethical standards

Conflict of interests

The authors declare that they do not have any conflict of interests.


  1. Abdulrahman L, Malki A, Sayed AAR (2013) Bees honey attenuation of metanil-yellow-induced hepatotoxicity in rats. Evid-Based Complement Altern Med 2013:614580Google Scholar
  2. Beers RF Jr, Sizer IW (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 195:133–140Google Scholar
  3. Benzie FF, Strain, JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Anal Biochem 239:70–76CrossRefGoogle Scholar
  4. Ellman GL (1959) Tissue sulphydryl groups. Arch Biochem Biophys 82:70–77CrossRefGoogle Scholar
  5. Jha V, Garcia G, Iseki K, Li Z, Naicker S, Plattner B, Saran R, Wang AY, Yang CW (2013) Chronic kidney disease: global dimension and perspectives. Lancet 382:260–272CrossRefGoogle Scholar
  6. Kumar S, Pandey AK (2015) Free radicals: health implications and their mitigation by herbals. Brit J Med Med Res 7:438–457CrossRefGoogle Scholar
  7. Kumar S, Kumar R, Dwivedi A, Pandey AK (2014) In vitro antioxidant, antibacterial and cytotoxic activity and in vivo effect of Syngonium podophyllum and Eichhornia crassipes leaf extracts on isoniazid induced oxidative stress and hepatic markers. Biomed Res Int 2014:459452Google Scholar
  8. Lee KC, Chan CC, Yang YY, Hsieh YC, Huang YH, Lin HC (2012) Aliskiren attenuates chronic carbon tetrachloride induced liver injury in mice. Eur J Clin Invest 42:1261–1271CrossRefGoogle Scholar
  9. Marklund S, Marklund G (1974) Involvement of superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47:469–474CrossRefGoogle Scholar
  10. Niehaus WG, Samuelsson B (1968) Formation of malondialdehyde from phospholipids archidonate during microsomal lipid peroxidation. Eur J Biochem 6:126–130CrossRefGoogle Scholar
  11. Ojha S, Venkataraman B, Kurdi A, Mahgoub E, Sadek S, Rajesh M (2016) Plant-derived agents for counteracting cisplatin induced nephrotoxicity. Oxid Med Cell Longev 2016:4320374Google Scholar
  12. Pandey AK, Mishra AK, Mishra A (2012) Antifungal and antioxidative potential of oil and extracts derived from leaves of indian spice plant Cinnamomum tamala. Cell Mol Biol 58:142–147Google Scholar
  13. Raouf OM, Sayed EM, Manie MM (2015) Cinnamic acid and cinnamaldehyde ameliorate cisplatin-induced splenotoxicity in rats. J Biochem Mol Toxicol 26:426–431CrossRefGoogle Scholar
  14. Saxena B, Sharma S (2014) Serological changes induced by blend of sunset yellow, metanil yellow and tartrazine in Swiss Albino rat, Rattus Norvegicus. Toxicol Int 21:65–68CrossRefGoogle Scholar
  15. Sharma UK, Sharma AK, Pandey AK (2016) Medicinal attributes of major phenylpropanoids present in cinnamon. BMC Complement Altern Med 16:156CrossRefGoogle Scholar
  16. Yamabe N, Song K, Lee W, Han I, Lee J, Ham Kim S, Park J, Kang KS (2012) Chemical and free radical-scavenging activity changes of ginsenoside by Maillard reaction and its possible use as a renoprotective agent. J Ginseq Res 36:256–262CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Uma Kant Sharma
    • 1
  • Ramesh Kumar
    • 1
  • Ashutosh Gupta
    • 1
  • Risha Ganguly
    • 1
  • Abhay K. Pandey
    • 1
  1. 1.Department of BiochemistryUniversity of AllahabadAllahabadIndia

Personalised recommendations