3 Biotech

, 8:206 | Cite as

Bioremediation of phenol from synthetic and real wastewater using Leptolyngbya sp.: a comparison and assessment of lipid production

  • Sohini Guha Thakurta
  • Mukesh Aakula
  • Jitamanyu Chakrabarty
  • Susmita Dutta
Original Article
  • 25 Downloads

Abstract

Bioremediation of wastewater is gaining popularity over chemical treatment due to the greener aspect. The volume of literature containing algal biodegradation is small. Especially, removal of toxic materials like phenol from coke-oven wastewater using fast-growing cyanobacteria was not tried. The current study, therefore, targeted at bioremediation of phenol from wastewater using Leptolyngbya sp., a cyanobacterial strain, as a finishing step. Furthermore, the growth of the strain was studied under different conditions, varying phenol concentration 50–150 mg/L, pH 5–11, inoculum size 2–10% to assess its ability to produce lipid. The strain was initially grown in BG-11 as a reference medium and later in phenolic solution. The strain was found to sustain 150 mg/L concentration of phenol. SEM study had shown the clear difference in the structure of cyanobacterial strain when grown in pure BG-11 medium and phenolic solution. Maximum removal of phenol (98.5 ± 0.14%) was achieved with an initial concentration 100 mg/L, 5% inoculum size at pH 11, while the maximum amount of dry biomass (0.38 ± 0.02 g/L) was obtained at pH 7, initial phenol concentration of 50 mg/L, and 5% inoculum size. Highest lipid yield was achieved at pH 11, initial phenol concentration of 100 mg/L, and 5% inoculum size. Coke-oven wastewater collected from secondary clarifier of effluent treatment plant was also treated with the said strain and the removal of different pollutants was observed. The study suggests the utilization of such potential cyanobacterial strain in treating industrial effluent containing phenol.

Keywords

Phenol Bioremediation Cyanobacteria Leptolynbga sp. Coke-oven wastewater 

Notes

Acknowledgements

This work is supported by Dept. of Chemistry, Dept. of Earth and Environmental Studies, and Dept. of Chemical Engineering, National Institute of Technology. We sincerely thank Gurpreet Kaur Wadhwa, M.Tech. student of Dept. of Earth and Environmental Studies, NIT Durgapur for helping with conducting the experiments.

Compliance with ethical standards

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

References

  1. Abdel RN, Homaidan Al AA, Ibraheem IBM (2012) Microalgae and wastewater treatment. Saudi J Biol Sci 19:257–275.  https://doi.org/10.1016/j.sjbs.2012.04.005 CrossRefGoogle Scholar
  2. Banat FA, Bashir Al B, Asheh Al S, Hayajneh O (2000) Adsorption of phenol by bentonite. Environ Pollu 107:391–398.  https://doi.org/10.1016/S0269-7491(99)00173-6 CrossRefGoogle Scholar
  3. Bligh EG, Dyer WM (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917CrossRefGoogle Scholar
  4. Bohdziewicz J, Kaminski G, Tytła M (2012) The Removal of Phenols from wastewater through sorption on activated carbon. Arch Civil Eng Environ 2:89–94Google Scholar
  5. Clesceri LS, Greenberg AE, Trussell RR (1996) Standard methods for the examination of water and wastewater. APHA, AWWA, WPCF, Washington DCGoogle Scholar
  6. Dakhil IH (2013) Removal of phenol from industrial wastewater using sawdust. Inter J Eng Sci 3:25–31Google Scholar
  7. Environmental standards. http://scclmines.com/env/Linkfile2.htm Accessed 08 June 2017
  8. Gao L, Li S, Wang Y, Sun H (2015) Organic pollution removal from coke plant wastewater using coking coal. Water Sci Technol 72:158–163.  https://doi.org/10.2166/wst.2015.197 CrossRefGoogle Scholar
  9. Ghasemi Y, Rasoulamini S, Naseri AT, Montazerinajafabady N, Mobasher MA, Dabbagh F (2012) Microalgae biofuel potentials (review). Appl Biochem Microbiol 48:126–144.  https://doi.org/10.1134/S0003683812020068 CrossRefGoogle Scholar
  10. Ghose MK (2002) Physico-chemical treatment of coke plant effluents for control of water pollution in India. Ind J Chem Technol 9:54–59Google Scholar
  11. Hangchang S (2009) Point sources of pollution: local effects and its control. Qian Yi, EOLSS, OxfordGoogle Scholar
  12. Issa OM, Defarge C, Bissonnais YL et al (2007) Effects of the inoculation of cyanobacteria on the microstructure and the structural stability of a tropical soil. Plant Soil 290:209–219.  https://doi.org/10.1007/s11104-006-9153-9 CrossRefGoogle Scholar
  13. Jain AK, Gupta VK, Jain S, Suhas (2004) Removal of chlorophenols using industrial wastes. Environ Sci Technol 38:1195–1200.  https://doi.org/10.1021/es034412u CrossRefGoogle Scholar
  14. Karn SK, Chakrabarti S (2015) Simultaneous biodegradation of organic (chlorophenols) and inorganic compounds from secondary sludge of pulp and paper mill by Eisenia fetida. Int J Recycl Org Waste Agricult 4:53–62.  https://doi.org/10.1007/s40093-015-0085-3 CrossRefGoogle Scholar
  15. Kulkarni SJ, Kaware JP (2013) Review on research for removal of phenol from wastewater. Int J Sci Res Publ 3:1–5Google Scholar
  16. Kundu N, Pal M, Saha S (2007) East Kolkata Wetlands: a resource recovery system through productive activities. In: Proceedings of Taal: the 12th world lake conference, pp 868–881Google Scholar
  17. Liu Z, Wenyu X, Dehao L, Yang P, Zesheng L, Shusi L (2016) Biodegradation of Phenol by Bacteria Strain Acinetobacter calcoaceticus PA Isolated from Phenolic Wastewater, Int J Environ Res Public Health 13:300–308.  https://doi.org/10.3390/ijerph13030300
  18. Mahiudddin Md, Fakhruddin AN, Abdullah Al M (2012) Degradation of phenol via meta-cleavage pathway by Pseudomonas fluorescens PU1. ISRN Microb 741820:1–6.  https://doi.org/10.5402/2012/741820 CrossRefGoogle Scholar
  19. Michałowicz J, Duda W (2007) Phenols—sources and toxicity. Pol J Environ Stud 16:347–362Google Scholar
  20. Phenol ambient water quality criteria. Office of the planning and standards Environmental Protection Agency (1979), EPA, Washington, DC. https://nepis.epa.gov. Accessed 08 June 2017
  21. Pinto G, Pollio A, Previtera L, Temussi F (2002) Biodegradation of phenols by microalgae. Biotechnol Lett 24:2047–2051.  https://doi.org/10.1023/A:1021367304315 CrossRefGoogle Scholar
  22. Pollio A, Pinto G, Ligrone R, Giovanni A (1993) Effects of the potential allelochemical α-asarone on growth, physiology and ultrastructure of two unicellular green algae. J Appl Phycol 5:395–403.  https://doi.org/10.1007/BF02182732 CrossRefGoogle Scholar
  23. Raychaudhuri S, Mishra M, Nandy P, Thakur A (2008) Waste management: a case study of ongoing traditional practices at East Calcutta. Am J Agri and Biol Sci 3:315–320.  https://doi.org/10.3844/ajabssp2008.315.320 CrossRefGoogle Scholar
  24. Sadhu K, Mukherjee A, Shukla Kr S, Adhikari K, Dutta S (2013) Adsorptive removal of phenol from coke-oven wastewater using Gondwana shale, India: experiment, modeling and optimization. Desalination Water Treat 52:6492–6504.  https://doi.org/10.1080/19443994.2013.815581 CrossRefGoogle Scholar
  25. Semple KT, Cain RB (1996) Biodegradation of phenols by the alga Ochromonas danica. Appl Environ Microbiol 62:1265–1273. (0099-2240/96/$04.0010)Google Scholar
  26. Sezos TM, Remoundaki E, Hatzikioseyian A (2007) Workshop on clean production and nano technologies. Seoul, KoreaGoogle Scholar
  27. Subashchandrabose SR, Ramakrishnan B, Megharaj M, Venkateswarlu K, Naidu R (2013) Mixotrophic cyanobacteria and microalgae as distinctive biological agents for organic pollutant degradation. Environ Int 51:59–72.  https://doi.org/10.1016/j.envint.2012.10.007 CrossRefGoogle Scholar
  28. Tian M, Du D, Zhou W, Zeng X, Cheng G (2017) Phenol degradation and genotypic analysis of dioxygenase genes in bacteria isolated from sediments. Braz J Microbiol 48:305–313.  https://doi.org/10.1016/j.bjm.2016.12.002 CrossRefGoogle Scholar
  29. Ullrich WR, Rigano C, Fuggi A, Aparicio PJ (Eds) (1990) Inorganic nitrogen in plants and microorganisms: uptake and metabolism. Springer, BerlinGoogle Scholar
  30. Wilberg KQ, Nunes DG, Rubio J (2000) Removal of phenol by enzymatic oxidation and flotation. Braz J Chem Eng 17:4–7.  https://doi.org/10.1590/S0104-66322000000400055 CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of ChemistryNational Institute of TechnologyDurgapurIndia
  2. 2.Department of Earth and Environmental StudiesNational Institute of TechnologyDurgapurIndia
  3. 3.Department of Chemical EngineeringNational Institute of TechnologyDurgapurIndia

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