3 Biotech

, 8:180 | Cite as

Effect of cytokinins on in vitro multiplication, volatiles composition and rosmarinic acid content of Thymus leucotrichus Hal. shoots

  • Tuba Bekircan
  • Ahmet Yaşar
  • Sercan Yıldırım
  • Münevver Sökmen
  • Atalay SökmenEmail author
Original Article


An efficient in vitro multiplication protocol was designed to Thymus leucotrichus, a subshrub and perennial herb growing naturally in the Northwest of Turkey. Of all basal media studied, Murashige and Skoog medium was found to be superior to the others, providing higher shoot formation and the maximum shoot length. Varying concentrations of cytokinins, i.e., 6-benzyladenine, thidiazuron, 2-isopentenyladenine and kinetin were supplemented in the nutrient media to observe their effects on shoot development and biomass. Rosmarinic acid content and volatile compositions of both naturally growing plants and in vitro multiplied plantlets were also evaluated. 6-benzyladenine (1.0 mg/L) and kinetin (0.5 mg/L) were found to be optimum for shoot number and shoot elongation, respectively. Thidiazuron (1.0 mg/L) was superior for biomass production. Rosmarinic acid content of in vitro multiplied plants was found to be higher than that of wild plants, reaching a maximum with 0.5 mg/L 2-isopentenyladenine, which yielded 10.15 mg/g dry weight. The highest thymol content was obtained with 1.0 mg/L kinetin (55.82%), while thidiazuron (0.1 mg/L) increased carvacrol production (12.53%). Overall, Murashige and Skoog medium supplemented with 1.0 mg/L kinetin was determined to be the most favorable medium studied.


Biomass Cytokinins Volatiles composition Multiplication Rosmarinic acid Thymus leucotrichus 



The authors thank King Saud University’s Distinguished Scientist Fellowship Program (DSFP) for financial support.

Compliance with ethical standards

Conflict of interest

Authors declare that there is no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Tuba Bekircan
    • 1
  • Ahmet Yaşar
    • 2
  • Sercan Yıldırım
    • 2
  • Münevver Sökmen
    • 3
    • 4
  • Atalay Sökmen
    • 3
    • 5
    Email author
  1. 1.Department of Biochemistry, Faculty of ScienceAvrasya UniversityTrabzonTurkey
  2. 2.Department of Analytical Chemistry, Faculty of PharmacyKaradeniz Technical UniversityTrabzonTurkey
  3. 3.Department of Zoology, College of ScienceKing Saud UniversityRiyadhSaudi Arabia
  4. 4.Department of Bioengineering, Faculty of EngineeringFood and Agriculture UniversityKonyaTurkey
  5. 5.Department of Plant Production and Technologies, Faculty of Natural SciencesFood and Agriculture UniversityKonyaTurkey

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