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3 Biotech

, 8:181 | Cite as

Extraction and characterization of collagen hydrolysates from the skin of Rana chensinensis

  • Yuanyuan Zhao
  • Zhanyong Wang
  • Jing Zhang
  • Tingting Su
Original Article
  • 42 Downloads

Abstract

The production of Hasma generates plentiful non-edible by-products in China and Central Asia. As one of main by-products, the skin of Rana chensinensis is discarded as waste without utilization. In this work, R. chensinensis skin collagen (RCSC) hydrolysates were extracted using pepsin under acidic conditions. The yield of RCSC is 15.1% (w/w). Amino acid analysis revealed that RCSC contained glycine (204.5/1000 residues) and imino acids (182/1000 residues). RCSC exhibited high solubility in acidic pH (1–4) and low NaCl concentrations (< 2%, w/v). Differential scanning calorimetry indicated that the denaturation temperature of RCSC was 33.5 °C. Scanning electron microscopy analysis confirmed their well-defined fibril morphologies. The results indicated that the skin of R. chensinensis is an alternative source of collagen hydrolysates, and RCSC can serve as a potential source applying in foodstuff and medical industry.

Keywords

Rana chensinensis Skin Collagen hydrolysates Extraction Characterization 

Notes

Acknowledgements

The authors gratefully acknowledge the financial supports by Program for Liaoning Excellent Talents in University (Grant no. LJQ2014040) and Science and Technology Development Foundation of Fushun City (Grant no. 20142101).

Compliance with ethical standards

Conflict of interest

All the authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Chemistry, Chemical Engineering and Environmental EngineeringLiaoning Shihua UniversityFushunChina

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