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In vitro and in silico characterization of a novel dextranase from Pochonia chlamydosporia

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Abstract

The objective of this study was to purify, characterize, and phylogenetically and structurally analyze the dextranase produced by the fungus Pochonia chlamydosporia. Dextranase produced by the fungus P. chlamydosporia was purified to homogeneity in two steps, with a yield of 152%, purification factor of 6.84 and specific activity of 358.63 U/mg. Its molecular weight was estimated by SDS-PAGE at 64 kDa. The enzyme presented higher activity at 50 °C and pH 5.0, using 100 mM citrate–phosphate buffer, was inhibited by Ag1+, Hg2+, Cu2+, Mg2+, and presented KM of 23.60 µM. Mature dextranase is composed of 585 amino acids residues, with a predicted molecular weight of 64.38 kDa and pI 5.96. This dextranase showed a strong phylogenetic similarity when compared to Trichoderma harzianum dextranase. Its structure consists of two domains: the first composed by 15 β strands, and the second composed by a right-handed parallel β-helix.

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Acknowledgements

The authors would like to thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), the Fundação de Amparo à Pesquisa de Minas Gerais (FAPEMIG), and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the financial support.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Av. Peter Henry Rolfs, s/n, Campus Universitário, Viçosa, Minas Gerais, 36570-000, Brazil

    Bruna Leite Sufiate, Filippe Elias de Freitas Soares, Samara Silveira Moreira, Angélica de Souza Gouveia & José Humberto de Queiroz

  2. Department of Animal Science, Universidade Federal do Espírito Santo, Alto Universitário, s/n, Guararema, Alegre, Espírito Santo, 29500-000, Brazil

    Evandro Ferreira Cardoso

  3. Universidade Vila Velha, Av. Comissário José Dantas de Melo, n° 21, Boa Vista, Vila Velha, Espírito Santo, 29102-920, Brazil

    Fabio Ribeiro Braga

  4. Department of Veterinary Medicine, Universidade Federal de Viçosa, Av. Peter Henry Rolfs, s/n, Campus Universitário, Viçosa, Minas Gerais, 36570-000, Brazil

    Jackson Victor de Araújo

  5. Scholarship CNPq, Brasília, Brazil

    Jackson Victor de Araújo

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  1. Bruna Leite Sufiate
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  2. Filippe Elias de Freitas Soares
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  3. Samara Silveira Moreira
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  4. Angélica de Souza Gouveia
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  5. Evandro Ferreira Cardoso
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  6. Fabio Ribeiro Braga
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  7. Jackson Victor de Araújo
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  8. José Humberto de Queiroz
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Correspondence to José Humberto de Queiroz.

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Sufiate, B.L., Soares, F.E.d., Moreira, S.S. et al. In vitro and in silico characterization of a novel dextranase from Pochonia chlamydosporia. 3 Biotech 8, 167 (2018). https://doi.org/10.1007/s13205-018-1192-4

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  • DOI: https://doi.org/10.1007/s13205-018-1192-4

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