3 Biotech

, 8:132 | Cite as

Optimization of silver nanoparticles biosynthesis mediated by Aspergillus niger NRC1731 through application of statistical methods: enhancement and characterization

  • Maysa A. Elsayed
  • Abdelmageed M. Othman
  • Mohamed M. Hassan
  • Ali M. Elshafei
Original Article


The fungal-mediated silver nanoparticles (AgNPs) biosynthesis optimization via the application of central composite design (CCD) response surface to develop an effective ecofriendly and inexpensive green process was the aim of the current study. Nanosilver biosynthesis using the Aspergillus niger NRC1731 cell-free filtrate (CFF) was studied through involving the most parameters affecting the AgNPs green synthesis and its interactions effects. The statistical optimization models showed that using 59.37% of CFF in reaction containing 1.82 mM silver nitrate for 34 h at pH 7.0 is the optimum value to optimize the AgNPs biosynthesis. The obtained AgNPs were characterized by means of electron microscopy, UV/visible spectrophotometry, energy dispersive X-ray analysis and infrared spectroscopy to elucidate its almost spherical shape with diameter of 3–20 nm. The produced AgNPs exhibited a considerable antimicrobial activity against Bacillus mycoides, Escherichia coli in addition to Candida albicans.


Nanosilver Aspergillus niger Biosynthesis Optimization Characterization 



Authors acknowledge the instrumental facilities and financial support provided by the National Research Centre (NRC), Dokki, Giza, Egypt.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Maysa A. Elsayed
    • 1
  • Abdelmageed M. Othman
    • 1
  • Mohamed M. Hassan
    • 1
  • Ali M. Elshafei
    • 1
  1. 1.Microbial Chemistry Department, Genetic Engineering and Biotechnology Research DivisionNational Research Centre (NRC)GizaEgypt

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