Advertisement

3 Biotech

, 8:132 | Cite as

Optimization of silver nanoparticles biosynthesis mediated by Aspergillus niger NRC1731 through application of statistical methods: enhancement and characterization

  • Maysa A. Elsayed
  • Abdelmageed M. Othman
  • Mohamed M. Hassan
  • Ali M. Elshafei
Original Article
  • 24 Downloads

Abstract

The fungal-mediated silver nanoparticles (AgNPs) biosynthesis optimization via the application of central composite design (CCD) response surface to develop an effective ecofriendly and inexpensive green process was the aim of the current study. Nanosilver biosynthesis using the Aspergillus niger NRC1731 cell-free filtrate (CFF) was studied through involving the most parameters affecting the AgNPs green synthesis and its interactions effects. The statistical optimization models showed that using 59.37% of CFF in reaction containing 1.82 mM silver nitrate for 34 h at pH 7.0 is the optimum value to optimize the AgNPs biosynthesis. The obtained AgNPs were characterized by means of electron microscopy, UV/visible spectrophotometry, energy dispersive X-ray analysis and infrared spectroscopy to elucidate its almost spherical shape with diameter of 3–20 nm. The produced AgNPs exhibited a considerable antimicrobial activity against Bacillus mycoides, Escherichia coli in addition to Candida albicans.

Keywords

Nanosilver Aspergillus niger Biosynthesis Optimization Characterization 

Notes

Acknowledgements

Authors acknowledge the instrumental facilities and financial support provided by the National Research Centre (NRC), Dokki, Giza, Egypt.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.

References

  1. AbdelRahim K, Mahmoud SY, Ali AM et al (2017) Extracellular biosynthesis of silver nanoparticles using Rhizopus stolonifer. Saudi J Biol Sci 24:208–216.  https://doi.org/10.1016/j.sjbs.2016.02.025 CrossRefGoogle Scholar
  2. Bagherzade G, Tavakoli MM, Namaei MH (2017) Green synthesis of silver nanoparticles using aqueous extract of saffron (Crocus sativus L.) wastages and its antibacterial activity against six bacteria. Asian Pac J Trop Biomed 7:227–233.  https://doi.org/10.1016/j.apjtb.2016.12.014 CrossRefGoogle Scholar
  3. Banach M, Pulit-Prociak J (2017) Proecological method for the preparation of metal nanoparticles. J Clean Prod 141:1030–1039.  https://doi.org/10.1016/j.jclepro.2016.09.180 CrossRefGoogle Scholar
  4. Devi LS, Joshi SR (2015) Ultrastructures of silver nanoparticles biosynthesized using endophytic fungi. J Microsc Ultrastruct 3:29–37.  https://doi.org/10.1016/j.jmau.2014.10.004 CrossRefGoogle Scholar
  5. Durán N, Marcato PD, Alves OL et al (2005) Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains. J Nanobiotechnol 3:8.  https://doi.org/10.1186/1477-3155-3-8 CrossRefGoogle Scholar
  6. El-Rafie MH, Shaheen TI, Mohamed AA, Hebeish A (2012) Bio-synthesis and applications of silver nanoparticles onto cotton fabrics. Carbohydr Polym 90:915–920.  https://doi.org/10.1016/j.carbpol.2012.06.020 CrossRefGoogle Scholar
  7. Fayaz AM, Balaji K, Girilal M et al (2010) Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: a study against gram-positive and gram-negative bacteria. Nanomed Nanotechnol Biol Med 6:103–109.  https://doi.org/10.1016/j.nano.2009.04.006 CrossRefGoogle Scholar
  8. Golinska P, Wypij M, Ingle AP et al (2014) Biogenic synthesis of metal nanoparticles from actinomycetes: biomedical applications and cytotoxicity. Appl Microbiol Biotechnol 98:8083–8097.  https://doi.org/10.1007/s00253-014-5953-7 CrossRefGoogle Scholar
  9. Gopinath V, MubarakAli D, Priyadarshini S et al (2012) Biosynthesis of silver nanoparticles from Tribulus terrestris and its antimicrobial activity: a novel biological approach. Colloids Surf B 96:69–74.  https://doi.org/10.1016/j.colsurfb.2012.03.023 CrossRefGoogle Scholar
  10. Helaly FM, El-Sawy SM, Hashem AI et al (2017) Synthesis and characterization of nanosilver-silicone hydrogel composites for inhibition of bacteria growth. Contact Lens Anterior Eye 40:59–66.  https://doi.org/10.1016/j.clae.2016.09.004 CrossRefGoogle Scholar
  11. Ibrahim HMM (2015) Green synthesis and characterization of silver nanoparticles using banana peel extract and their antimicrobial activity against representative microorganisms. J Radiat Res Appl Sci 8:265–275.  https://doi.org/10.1016/j.jrras.2015.01.007 CrossRefGoogle Scholar
  12. Kumari RM, Thapa N, Gupta N et al (2016) Antibacterial and photocatalytic degradation efficacy of silver nanoparticles biosynthesized using Cordia dichotoma leaf extract. Adv Nat Sci Nanosci Nanotechnol 7:045009.  https://doi.org/10.1088/2043-6262/7/4/045009 CrossRefGoogle Scholar
  13. Lu S-Y, Qian J-Q, Wu Z-G et al (2009) Application of statistical method to evaluate immobilization variables of trypsin entrapped with sol-gel method. J Biochem Tech 1:79–84Google Scholar
  14. Manjari Mishra P, Kumar Sahoo S, Kumar Naik G, Parida K (2015) Biomimetic synthesis, characterization and mechanism of formation of stable silver nanoparticles using Averrhoa carambola L. leaf extract. Mater Lett 160:566–571.  https://doi.org/10.1016/j.matlet.2015.08.048 CrossRefGoogle Scholar
  15. McGillicuddy E, Murray I, Kavanagh S et al (2017) Silver nanoparticles in the environment: sources, detection and ecotoxicology. Sci Total Environ 575:231–246.  https://doi.org/10.1016/j.scitotenv.2016.10.041 CrossRefGoogle Scholar
  16. Misra A, Tyagi PK, Singh MK, Misra DS (2006) FTIR studies of nitrogen doped carbon nanotubes. Diam Relat Mater 15:385–388.  https://doi.org/10.1016/j.diamond.2005.08.013 CrossRefGoogle Scholar
  17. Mittal AK, Bhaumik J, Kumar S, Banerjee UC (2014) Biosynthesis of silver nanoparticles: elucidation of prospective mechanism and therapeutic potential. J Colloid Interface Sci 415:39–47.  https://doi.org/10.1016/j.jcis.2013.10.018 CrossRefGoogle Scholar
  18. Othman AM, Elsayed MA, Elshafei AM, Hassan MM (2016) Nano-silver biosynthesis using culture supernatant of Penicillium politans NRC510: optimization, characterization and its antimicrobial activity. Int J ChemTech Res 9:433–444Google Scholar
  19. Othman AM, Elsayed MA, Elshafei AM, Hassan MM (2017) Application of response surface methodology to optimize the extracellular fungal mediated nanosilver green synthesis. J Genet Eng Biotechnol 15:497–504.  https://doi.org/10.1016/j.jgeb.2017.08.003 CrossRefGoogle Scholar
  20. Pugazhendhi A, Prabakar D, Jacob JM et al (2018) Synthesis and characterization of silver nanoparticles using Gelidium amansii and its antimicrobial property against various pathogenic bacteria. Microb Pathog 114:41–45.  https://doi.org/10.1016/j.micpath.2017.11.013 CrossRefGoogle Scholar
  21. Pulit J, Banach M (2014) Preparation of nanosilver and nanogold based on dog rose aqueous extract. Bioinorg Chem Appl https://www.hindawi.com/journals/bca/2014/658935/. Accessed 3 Dec 2017
  22. Pulit J, Banach M, Zielina M, et al (2013) Raspberry extract as both a stabilizer and a reducing agent in environmentally friendly process of receiving colloidal silver. J Nanomater https://www.hindawi.com/journals/jnm/2013/563826/. Accessed 3 Dec 2017
  23. Rivera-Rangel RD, González-Muñoz MP, Avila-Rodriguez M et al (2018) Green synthesis of silver nanoparticles in oil-in-water microemulsion and nano-emulsion using geranium leaf aqueous extract as a reducing agent. Colloids Surf Physicochem Eng Asp 536:60–67.  https://doi.org/10.1016/j.colsurfa.2017.07.051 CrossRefGoogle Scholar
  24. Singh D, Rathod V, Ninganagouda S et al (2014) Optimization and characterization of silver nanoparticle by endophytic fungi Penicillium sp. Isolated from Curcuma longa (Turmeric) and Application Studies against MDR E. coli and S. aureus. Bioinorg Chem Appl 2014:1–8.  https://doi.org/10.1155/2014/408021 Google Scholar
  25. Sintubin L, Verstraete W, Boon N (2012) Biologically produced nanosilver: current state and future perspectives. Biotechnol Bioeng 109:2422–2436.  https://doi.org/10.1002/bit.24570 CrossRefGoogle Scholar
  26. Sökmen M, Alomar SY, Albay C, Serdar G (2017) Microwave assisted production of silver nanoparticles using green tea extracts. J Alloys Compd 725:190–198.  https://doi.org/10.1016/j.jallcom.2017.07.094 CrossRefGoogle Scholar
  27. Veisi H, Azizi S, Mohammadi P (2018) Green synthesis of the silver nanoparticles mediated by Thymbra spicata extract and its application as a heterogeneous and recyclable nanocatalyst for catalytic reduction of a variety of dyes in water. J Clean Prod 170:1536–1543.  https://doi.org/10.1016/j.jclepro.2017.09.265 CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Maysa A. Elsayed
    • 1
  • Abdelmageed M. Othman
    • 1
  • Mohamed M. Hassan
    • 1
  • Ali M. Elshafei
    • 1
  1. 1.Microbial Chemistry Department, Genetic Engineering and Biotechnology Research DivisionNational Research Centre (NRC)GizaEgypt

Personalised recommendations