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Extruded polysaccharide/protein matrix from Arthrospira platensis cultures mediated silver nanoparticles biosynthesis and capping

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Abstract

Herein, Silver nanoparticles (AgNPs) have been synthesized and capped using secreted polysaccharide-protein matrix (EPS) from Arthrospira platensis cultures (A-EPS) and compared with EPS extracted from 1.0 K Gy gamma-irradiated A. platensis cultures (IA-EPS). Gamma irradiation enhanced EPS secretion and modified its biochemical composition. A. platensis cultures have been exposed to two acute gamma irradiation doses of 1.0–2.0 K Gy, EPS secretion was scaled up in response to gamma irradiation from 0.559 g L−1 at non-irradiated culture to 0.752, 0.690 g L−1 at 1.0–2.0 K Gy, respectively. Saccharide-protein nature of EPSs was confirmed via chemical, UV Visible spectrophotometer, and Fourier-transform infrared spectroscopy (FT-IR), revealed an increment of protein content in (IA-EPS). Hetero-polymeric constituents were elucidated using HPLC analysis which revealed seven monomers in the saccharide portion of (IA-EPS). AgNP biosynthesis was fabricated in alkaline medium whereas IA-EPS-mediated AgNPs (IEPS-AgNPs) exhibited a stronger UV absorbance than A-EPS-mediated AgNPs (EPS-AgNPs) at pH 9.0 and 2.0 mg L−1. Synthesized AgNPs displayed the difference in coating nature and its aggregation under Transmission Electron Microscope (TEM) whereas (IEPS-AgNPs) displayed mono-dispersed nanoparticles capped by protein layers meanwhile (EPS-AgNPs) exhibited poly-dispersed ones capped with a faint sheath of EPS. FT-IR analysis illustrated the capping mechanism for both EPS-AgNPs and IEPS-AgNPs.

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  1. Department of Biology, Faculty of Sciences and Arts Khulais, University of Jeddah, Jeddah, Kingdom of Saudi Arabia

    Ragaa A. Hamouda

  2. Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt

    Ragaa A. Hamouda

  3. Department of Botany, Faculty of Science, Mansoura University, Mansoura, Egypt

    Mervat H. Hussein & Muhammad A. Abuelmagd

  4. Biological Application Dept, Nuclear Research Center, Atomic Energy Authority, Cairo, Egypt

    Abdel Monsef A. Elhadary

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Hamouda, R.A., Hussein, M.H., Elhadary, A.M.A. et al. Extruded polysaccharide/protein matrix from Arthrospira platensis cultures mediated silver nanoparticles biosynthesis and capping. Appl Nanosci 10, 3839–3855 (2020). https://doi.org/10.1007/s13204-020-01490-z

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