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Gold nanoparticles as a factor of influence on doxorubicin–bovine serum albumin complex

  • N. A. Goncharenko
  • O. L. Pavlenko
  • O. P. Dmytrenko
  • M. P. Kulish
  • A. M. Lopatynskyi
  • V. I. Chegel
Original Article

Abstract

The interaction between doxorubicin (Dox) and bovine serum albumin (BSA) complex with gold nanoparticles (AuNPs) was investigated by optical spectroscopy. The optical absorption of Dox and BSA solutions was studied. The formation of Dox–BSA complexes with a binding constant K = 7.56 × 106 M−2 and the number of binding sites n = 2 was found out. With pH 6.9, the concentration of complexes is an order of magnitude lower than the concentration of unbound antibiotic molecules. Optical absorption in solutions of Dox–BSA conjugates in the presence of AuNPs undergoes a significant rearrangement, which manifests the changes in the magnitude of the hydrophobic interaction of BSA with Dox, changes in the conformational state of antibiotic, and, as a consequence, a plasmon-induced change in the mechanism of complex formation. The aggregation of the Dox–AuNPs conjugate depends on the presence and concentration of BSA and in the case of formation of the Dox–BSA complex is minimal.

Keywords

Doxorubicin Bovine serum albumin Gold nanoparticles Conformational state Hydrophobic interaction Electrostatic interaction Localized surface plasmon resonance 

Notes

Compliance with ethical standards

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • N. A. Goncharenko
    • 1
  • O. L. Pavlenko
    • 1
  • O. P. Dmytrenko
    • 1
  • M. P. Kulish
    • 1
  • A. M. Lopatynskyi
    • 2
    • 3
  • V. I. Chegel
    • 2
    • 3
  1. 1.Faculty of PhysicsTaras Shevchenko National University of KyivKievUkraine
  2. 2.V.E. Lashkaryov Institute of Semiconductor Physics, National Academy of Sciences of UkraineKievUkraine
  3. 3.Institute of High TechnologiesTaras Shevchenko National University of KyivKievUkraine

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