Towards single crystalline, highly monodisperse and catalytically active gold nanoparticles capped with probiotic Lactobacillus plantarum derived lipase

Original Article
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Abstract

Owing to the eco-friendly nature of biomolecules, there lies a huge interest in exploring them as capping agents for nanoparticles to achieve stability and biocompatibility. Lipase extracted from the probiotic Lactobacillus plantarum is utilized for the first time to study its efficacy in capping gold nanoparticles (GNPs) in the room temperature synthesis using HAuCl4. The synthesized lipase-capped GNPs are characterized using UV–visible spectroscopy, FT-IR, HR-TEM, DLS and zeta potential measurements. Importantly, selected area electron diffraction (SAED) studies with HR-TEM have revealed the effect of lipase capping in tuning the polycrystallinity of the GNPs. The lipase-capped GNPs are explored for their catalytic efficiency towards an environmentally and industrially important conversion of 4-nitrophenol to 4-aminophenol. Exploiting the amine functional groups in the protein, the recoverability and reusability of the GNPs have been demonstrated through immobilization over amine-functionalized Fe3O4 nanoparticles.

Keywords

Protein-capped gold nanoparticles Lactobacillus plantarum lipase Crystallinity of gold nanoparticles 4-Nitrophenol reduction Magnetic recoverability 

Notes

Acknowledgements

The authors would like to thank BITS, Pilani Hyderabad campus for their financial support. All technical staff in the Biological Sciences department and Central Analytical Laboratory of BITS, Hyderabad campus, are greatly acknowledged for their kind assistance.

Supplementary material

13204_2018_735_MOESM1_ESM.docx (1.5 mb)
Supplementary material 1 (DOCX 1515 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Biological SciencesBITS Pilani, Hyderabad CampusHyderabadIndia
  2. 2.Department of ChemistryBITS Pilani, Hyderabad CampusHyderabadIndia

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