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Towards single crystalline, highly monodisperse and catalytically active gold nanoparticles capped with probiotic Lactobacillus plantarum derived lipase

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Abstract

Owing to the eco-friendly nature of biomolecules, there lies a huge interest in exploring them as capping agents for nanoparticles to achieve stability and biocompatibility. Lipase extracted from the probiotic Lactobacillus plantarum is utilized for the first time to study its efficacy in capping gold nanoparticles (GNPs) in the room temperature synthesis using HAuCl4. The synthesized lipase-capped GNPs are characterized using UV–visible spectroscopy, FT-IR, HR-TEM, DLS and zeta potential measurements. Importantly, selected area electron diffraction (SAED) studies with HR-TEM have revealed the effect of lipase capping in tuning the polycrystallinity of the GNPs. The lipase-capped GNPs are explored for their catalytic efficiency towards an environmentally and industrially important conversion of 4-nitrophenol to 4-aminophenol. Exploiting the amine functional groups in the protein, the recoverability and reusability of the GNPs have been demonstrated through immobilization over amine-functionalized Fe3O4 nanoparticles.

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Acknowledgements

The authors would like to thank BITS, Pilani Hyderabad campus for their financial support. All technical staff in the Biological Sciences department and Central Analytical Laboratory of BITS, Hyderabad campus, are greatly acknowledged for their kind assistance.

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Author notes

  1. Imran Khan and Ravikiran Nagarjuna contributed equally.

Authors and Affiliations

  1. Department of Biological Sciences, BITS Pilani, Hyderabad Campus, Jawahar Nagar, Shameerpet Mandal, Hyderabad, 500078, Telangana, India

    Imran Khan & Jayati Ray Dutta

  2. Department of Chemistry, BITS Pilani, Hyderabad Campus, Jawahar Nagar, Shameerpet Mandal, Hyderabad, 500078, Telangana, India

    Ravikiran Nagarjuna & Ramakrishnan Ganesan

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  1. Imran Khan
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  2. Ravikiran Nagarjuna
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  3. Jayati Ray Dutta
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  4. Ramakrishnan Ganesan
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Correspondence to Jayati Ray Dutta or Ramakrishnan Ganesan.

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Khan, I., Nagarjuna, R., Ray Dutta, J. et al. Towards single crystalline, highly monodisperse and catalytically active gold nanoparticles capped with probiotic Lactobacillus plantarum derived lipase. Appl Nanosci 9, 1101–1109 (2019). https://doi.org/10.1007/s13204-018-0735-7

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