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Bio-hybridization of nanobactericides with cellulose films for effective treatment against members of ESKAPE multi-drug-resistant pathogens

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Abstract

The rapid expansion of drug-resistant pathogens has created huge global impact and development of novel antimicrobial leads is one of the top priority studies in the current scenario. The present study aims to develop bio-hybridized nanocellulose films which comprise of phytogenic silver nanobactericides. The nanobactericides were synthesized by treating 1 mM silver nitrate with aqueous extract of Chamerion angustifolium which reduced the metal salt to produce polydispersed nanobactericides which were tested against the members of ESKAPE drug-resistant communities. The synthesized silver nanobactericides were subjected to characterization with UV–visible spectra which displayed maximum absorbance at 408 nm. The bio-molecular interaction of phyto-constituents to mediate synthesis and stabilization of nanobactericides was studied with Fourier-transform infrared spectroscopy (FTIR) which depicted functional groups associated with nanobactericides. The crystalline nature was studied with X-ray diffraction (XRD) which showed Bragg’s intensities at 2θ angle which denoted (111), (200), (220), and (311) planes. The morphological characteristics of silver nanobactericides were defined with transmission electron Microscopy (TEM) image which displayed polydispersity of silver nanobactericides with size ranging from 2 to 40 nm. The synthesized nanobactericides showed a significant activity against MRSA strain with 21 mm zone of inhibition. The minimal inhibitory concentration of silver nanobactericides to inhibit the growth of test pathogens was also determined which ranged between 0.625 and 1.25 μg/ml. The silver nanobactericides were bio-hybridized onto nanocellulose films produced by Komagataeibacter xylinus B-12068 culture strain. The films were dried to determine the mechanical properties which showed increased in Young’s modulus and tensile strength in comparison with control bacterial cellulose films. Overall, the results obtained in the present investigation are promising enough to report bactericidal activity of bio-hybridized nanobactericidal films against ESKAPE. These communities are reported to cause severe threats to all forms of lives irrespective to their habitats which can lead to huge economical crisis.

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Acknowledgements

Authors are thankful for Ministry of Education and Science of the Russian Federation for providing funding under the scheme of 5-100: Russian Academic Excellence Project. Authors are grateful for facilities provided by Siberian Federal University to carry out the present study.

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Authors and Affiliations

  1. Laboratory of Biotechnology of New Materials, Siberian Federal University, Svobodnyy pr., 79, Krasnoyarsk, 660041, Siberia, Russia

    Syed Baker, Kondratenka Olga & Kiryukhin Bogdan

  2. Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russia

    Tatiana Volova

  3. Siberian Federal University, 79 Svobodny pr, Krasnoyarsk, 660041, Russia

    Tatiana Volova, Anna A. Shumilova, Sergey M. Zharkov & Ivan P. Shidlovskiy

  4. School of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russia

    Svetlana V. Prudnikova

  5. Department of Microbiology, Krasnoyasrk State Medical University named after Prof. VF. Voino-Yasenetskiy, Krasnoyarsk Partizana-Zheleznyaka street, 1, Krasnoyarsk, 660022, Russia

    Olga V. Perianova, Zoya K. Potkina, Olga Y. Khohlova & Tatiana I. Lobova

  6. Kirensky Institute of Physics, Federal Research Center KSC SB RAS, Akademgorodok 50, Bld. 38, Krasnoyarsk, 660036, Russia

    Sergey M. Zharkov

  7. School of Petroleum and Natural Gas Engineering, Siberian Federal University, Krasnoyarsk, Russia

    Andrey Kuzmin

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  1. Syed Baker
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Correspondence to Syed Baker.

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Baker, S., Volova, T., Prudnikova, S.V. et al. Bio-hybridization of nanobactericides with cellulose films for effective treatment against members of ESKAPE multi-drug-resistant pathogens. Appl Nanosci 8, 1101–1110 (2018). https://doi.org/10.1007/s13204-018-0717-9

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