Evaluation of phosphate Solubilization by root endophytic aquatic Hyphomycete Tetracladium setigerum

Abstract

The endophytic aquatic fungus Tetracladium setigerum Grove (Ingold) isolated from healthy roots of Berberris vulgaris growing in a riparian area was screened for its phosphate solubilization potential. This was estimated by a solubilization index (SI) using Pikovskaya’s (PVK) agar medium. Solubilized phosphate was quantified with the help of PVK broth medium. The Solubilization index of T. setigerum was ranged from 1.33 to 1.50 during the seven days of incubation and the highest soluble P (3.51 mg/L) was recorded in PVK broth after 21 days of incubation. The present study indicates that the studied root endophytic aquatic fungus may be a promising source of bio-fertilizer.

This is a preview of subscription content, log in to check access.

Plate 1
Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  1. Ames BN (1964) Assay of inorganic phosphate, total phosphate and phosphatases. Methods Enzymol 8: 115–118

    Article  Google Scholar 

  2. Arya P, Sati SC (2011) Evaluation of endophytic hyphomycetes for their antagonistic activity against pathogenic bacteria. Int Res J Microbiol 2:343–347 http://www.interesjournals.org/IRJM

    Google Scholar 

  3. Banik S, Dey BK (1982) Available phosphate content of an alluvial soil as influenced by inoculation of some isolated phosphate solubilizing microorganisms. Plant Soil 69:353–364 0032-079X/82/0693-0353501.80

    CAS  Article  Google Scholar 

  4. Bills GE, Polishok JD (1992) Recovery of endophytic fungi from Chamaecyparis thyoides. Sydowia 44:1–12 https://www.zobodat.at/pdf/Sydowia_44_0001-0012.pdf

    Google Scholar 

  5. Cunningham J, Kuiack C (1992) Production of citric and oxalic acids and solubilization of calcium phosphate by Penicillium billai. Appl Enviorn Microbiol 58:1451–1458 https://www.ncbi.nlm.nih.gov/pubmed/162221

    CAS  Google Scholar 

  6. Descals E (2005) Diagnostic characters of propagules of Ingoldian fungi. Mycol Res 101: 545-555

    Article  Google Scholar 

  7. Descals E, Webster J (1982) Taxonomic studies on “aquatic hyphomycetes” III some new species and a new combination. Trans Brit Mycol Soc 78:405–437. https://doi.org/10.1016/S0007-1536(82)80149-6

    Article  Google Scholar 

  8. Elias F, Woyessa D, Muleta D (2016) Phosphate solubilization potential of rhizosphere fungi isolated from plants in Jimma Zone, Southwest Ethiopia. Int J Microbiol Article ID 5472601, 11 pages https://doi.org/10.1155/2016/5472601

    Article  Google Scholar 

  9. Fankem H, Nwaga D, Deubel A, Dieng L, Merbach W, Etoa FX (2006) Occurrence and functioning of phosphate solubilizing microorganisms from oil palm tree (Elaeis guineensis) rhizosphere in Cameroon. Afr J Biotechnol 5(24):2450–2460 https://www.ajol.info/index.php/ajb/article/view/56044

    CAS  Google Scholar 

  10. Gulis VI, Suberkropp K (2003) Interactions between stream fungi and bacteria associated with decomposing leaf litter at different levels of nutrient availability. Aquatic Microbiol Ecol 30:149–157. https://doi.org/10.3354/ame030149

    Article  Google Scholar 

  11. Hara FAS, Oliveira LA (2005) Physiological and ecological characteristics of rhizobia isolates from acid soils of Iranduba, Amazonas. Pesq Agropec Bras 40:667–672. https://doi.org/10.1590/S0100-204X2005000700007

    Article  Google Scholar 

  12. Illmer P, Schinner F (1995) Phosphate solubililizing microorganisms under nonsterile conditions. Bodenkultur 46:197–204 https://diebodenkultur.boku.ac.at/volltexte/band-46/heft-1/illmer.pdf

    Google Scholar 

  13. Ingold CT (1942) Aquatic Hyphomycetes of decaying alder leaves. Trans Brit Mycol Soc 25:339–417. https://doi.org/10.1016/S0007-1536(42)80001-7

    Article  Google Scholar 

  14. Iyamuremye F, Dick RP (1996) Organic amendments and phosphorus sorption by soil. Adv Agron 56:139–185. https://doi.org/10.1016/S0065-2113(08)60181-9

    CAS  Article  Google Scholar 

  15. Jain R, Saxena J, Sharma V (2010) The evaluation of free and encapsulated A. awamori for phosphate solubilization in fermentation and soil-plant system. Appl Soil Ecol 46:90–94. https://doi.org/10.1016/j.apsoil.2010.06.008

    Article  Google Scholar 

  16. Kucey RMN (1983) Phosphate solubilizing bacteria and fungi in various cultivated and virgin Alberta soils. Can J Soil Sci 63:671–678. https://doi.org/10.4141/cjss83-068

    CAS  Article  Google Scholar 

  17. Kucey RMN (1987) Increased phosphorus uptake by wheat and field beans inoculated with a phosphorus solubihzing Penicillium bilaji strain and vesicular arbuscular mycorrhizal fungi. Appl Environ Microbiol 53: 2699–2703

    CAS  PubMed  PubMed Central  Google Scholar 

  18. Mittal V, Singh O, Nayyar H, Kaur J Tewari R (2008) Stimulatory effect of phosphate-solubilizing fungal strains (Aspergillus awamori and Penicilliumc itrinum) on the yield of chickpea (Cicer arietinum L. cv. GPF2). Soil Biol Biochem 40(3):718–727. https://doi.org/10.1016/j.soilbio.2007.10.008

    CAS  Article  Google Scholar 

  19. Murphy J, Riley HP (1962) A modified single solution method for the determination of phosphate in natural waters. Anal Chim Acta 27:31–36. https://doi.org/10.1016/S0003-2670(00)88444-5

    CAS  Article  Google Scholar 

  20. Oberson A, Friesen DK, Rao IM, Buhler S, Frossard E (2001) Phosphorus transformations in an oxisol under contrasting land-use system: the role of the microbial biomass. Plant Soil 237:197–210. https://doi.org/10.1023/A:1013301716913

    CAS  Article  Google Scholar 

  21. Omar SA (1998) The role of rock-phosphate-solubilizing fungi and vesicular-arbusular-mycorrhiza (VAM) in growth of wheat plants fertilized with rock phosphate. World J Microbiol Biotech 14:211–218. https://doi.org/10.1023/A:1008830129262

    CAS  Article  Google Scholar 

  22. Pradhan N, Sukla LB (2005) Solubilization of inorganic phosphates by fungi isolated from agriculture soil. Afr J Biotecnol 5:850–855

    Google Scholar 

  23. Quilliam RS, Jones DL (2010) Fungal root endophytes of the carnivorous plant Drosera rotundifolia. Mycorrhiza 20:341–348. https://doi.org/10.1007/s00572-009-0288-4

    Article  PubMed  Google Scholar 

  24. Sati SC, Arya P (2010a) Antagonism of some aquatic hyphomycetes against plant pathogenic fungi. Sci World J 10:760–765. https://doi.org/10.1100/tsw.2010.80

    CAS  Article  Google Scholar 

  25. Sati SC, Arya P (2010b) Assessment of root endophytic aquatic hyphomycetous fungi on plant growth. Symbiosis 50:143–149. https://doi.org/10.1100/tsw.2010.80

    Article  Google Scholar 

  26. Sati SC, Belwal M (2005) Aquatic hyphomycetes as endophyte of riparian plant roots. Mycologia 97:45–49. https://doi.org/10.1080/15572536.2006.11832837

    CAS  Article  PubMed  Google Scholar 

  27. Sati SC, Pant N, Belwal M (2002) Conidial aquatic fungi of Kumaun Himalaya, India. Mycotaxon 80:445–455 http://www.mycotaxon.com/vol/abstracts/81/81.445.html

    Google Scholar 

  28. Sati SC, Arya P, Belwal M (2009) Tetracladium nainitalense sp. nov, a root endophyte from Kumaun Himalaya, India. Mycologia 101(5):692–695. https://doi.org/10.3852/08-192

    CAS  Article  PubMed  Google Scholar 

  29. Sharma S, Kumar, Tripathi RB (2011) Isolation of phosphate solubilizing microorganism (PSMs) from soil. J Microbiol Biotechnol Res 1(2):90–95 https://jmbronline.com/index.php/JMBR/article/view/22

    Google Scholar 

  30. Sridhar KR, Bärlocher F (1992) Aquatic hyphomycetes in spruce roots. Mycologia 84:580–584. https://doi.org/10.2307/3760325

    Article  Google Scholar 

  31. Vassileva M, Serrano M, Bravo V, Jurado E, Nikolaeva I, Martos V, Vassilev N (2010) Multifunctional properties of phosphate-solubilizing microorganisms grown on agro-industrial wastes in fermentation and soil conditions. Appl Microbiol Biotechnol 85:1287–1299. https://doi.org/10.1007/s00253-009-2366-0

    CAS  Article  PubMed  Google Scholar 

  32. Whitelaw MA (2000) Growth promotion of plants inoculated with phosphate solubilizing fungi. In: Advances in Agronomy (Ed. Donald L. Sparks). Academic press. 69: 99–151

Download references

Acknowledgements

We are thankful to the University Grant Commission, New Delhi for financial support under DRS-SAP (UGC) scheme. Thanks are also due to the Head, Department of Botany, D.S.B. Campus, Kumaun University, Nainital, India for providing necessary lab facilities.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Prabha Pant.

Ethics declarations

Disclosure

The authors declare no conflicts of interest. Each author has equal share of contribution for this paper.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Sati, S.C., Pant, P. Evaluation of phosphate Solubilization by root endophytic aquatic Hyphomycete Tetracladium setigerum. Symbiosis 77, 141–145 (2019). https://doi.org/10.1007/s13199-018-0575-y

Download citation

Keywords

  • Aquatic hyphomycetes
  • Endophyte
  • T. setigerum
  • Phosphate solubilization
  • Biofertilizer