Abstract
Tuber species are hypogeous mycorrhizal symbionts distributed almost worldwide and associated with several tree families. In this study, we recorded the occurrence of two Tuber species (clade Puberulum) in the Acatlán Volcano (Veracruz, Eastern Mexico). They were detected by the means of the internal transcribed spacer (ITS) of nuclear ribosomal DNA sequences obtained from ectomycorrhizal samples collected at the site. The Tuber ectomycorrhizas were related symbiotically with Alnus acuminata and Fagus grandifolia var. mexicana. This is the first report of Tuber associated with these trees. The anatomical and morphological characterization of the ectomycorrhizae are presented along with illustrations.
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Acknowledgements
We appreciate the assistance in the field and in the laboratory by Biols. D. Ramos and J.C. Corona (both at Instituto de Ecología, A.C.). We recognize the support given by CONACYT (CB 169172) to study the EcM fungi associated with Fagus grandifolia var. mexicana in the montane cloud forest of Central Veracruz and by the postdoctoral grant to A. Ramos. We acknowledge the support given by CONACYT (225382) to the Laboratorio de Presecuenciación, Red Biodiversidad y Sistemática, INECOL. M.Sc. Bertha Pérez at this lab aid in some molecular procedures. We are thankful to the anonymous reviewers and the editor, Professor David Richardson, for their helpful suggestions on the manuscript.
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Ramos, A., Garay-Serrano, E., César, E. et al. Ectomycorrhizas of two species of Tuber (clade Puberulum) in the Mexican subtropical cloud forest. Symbiosis 76, 1–12 (2018). https://doi.org/10.1007/s13199-017-0535-y
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DOI: https://doi.org/10.1007/s13199-017-0535-y