, Volume 75, Issue 1, pp 61–67 | Cite as

Salinity induced changes in the chloroplast proteome of the aquatic pteridophyte Azolla microphylla

  • Preeti Thagela
  • Ravindra Kumar Yadav
  • Keshawanand Tripathi
  • Pawan Kumar Singh
  • Altaf Ahmad
  • Anil Dahuja
  • Gerard Abraham


The growth of the nitrogen fixing aquatic pteridophyte Azolla microphylla is severely affected by salinity. Salinity exposure (0.5%) resulted in significant reduction in chlorophyll a and b content, altered chl a/b ratio and photosynthetic efficiency (Fv/Fm). Chloroplasts maintain photosynthesis but are highly sensitive to salinity stress. Chloroplast proteins extracted from A. microphylla was separated by two-dimensional electrophoresis (2DE) and approximately 200 proteins were observed on each gel. Forty two differentially expressed protein spots were detected and out of this 17 could be identified through MALDI-TOF-MS/MS analysis. Out of the 17 identified proteins, 15 were found to be down regulated and 2 proteins were up regulated. Most of the down regulated proteins were associated with Calvin cycle, ATP synthesis, oxygen evolution, photosystem I and ROS scavenging. The results show changes in proteome dynamics of the chloroplasts of A. microphylla and such changes may lead to reduction in growth and metabolism. The primary target of salinity in A. microphylla is photosynthesis and the changes in the proteome dynamics of the chloroplasts lead to reduced growth.


A. microphylla Chloroplast Photosynthesis Proteomics Salinity 



Financial assistance (SR/SO/PS/0077/2010) from the Science and Engineering Research Board (SERB), Ministry of Science and Technology, Government of India, New Delhi is gratefully acknowledged. Preeti Thagela acknowledges SERB for Junior and Senior Research Fellowship from SERB. Necessary facilities provided by the Head, Division of Microbiology, ICAR-IARI, New Delhi is greatly acknowledged.

Author contributions

The experiments reported in the manuscript were planned by GA, AD, AA and PKS and they also provided the necessary intellectual in puts. Sample preparation for the 2D gel electrophoresis work and photosynthetic yield was performed by PT and RKY and K.N.T. assisted in the analysis. Software analysis of the data was conducted by PT and RKY.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

13199_2017_521_MOESM1_ESM.docx (1.7 mb)
ESM 1 (DOCX 1761 kb)


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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Preeti Thagela
    • 1
  • Ravindra Kumar Yadav
    • 1
  • Keshawanand Tripathi
    • 1
  • Pawan Kumar Singh
    • 2
  • Altaf Ahmad
    • 3
  • Anil Dahuja
    • 4
  • Gerard Abraham
    • 1
  1. 1.Centre for Conservation and Utilization of BGAICAR-Indian Agricultural Research InstituteNew DelhiIndia
  2. 2.Department of BotanyBanaras Hindu UniversityVaranasiIndia
  3. 3.Department of BotanyAligarh Muslim UniversityAligarhIndia
  4. 4.Division of BiochemistryICAR-Indian Agricultural Research InstituteNew DelhiIndia

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