FT-IR fingerprinting as an Analytical tool for determination of Melamine leaching from Melamine tablewares and their Biological implications

Abstract

In recent years Melamine is upgraded as a type three carcinogen. It is a sorry fact that still; people are in the fantasy mode and abaft the melamine tableware as they are a good piece of decorative material which sets dining an opulent look. The present study focuses on the determination of melamine leaching from melamine tablewares. The food stimulants culled for this study are conventional Indian cuisines. FT-IR is used as an analytical tool to determine the leaching of melamine from melamine diners. The present study reveals melamine leaching when hot food articles are in contact with melamine wares. Microwave heating is unsuitable for melamine tablewares. This is the first report in India, on the leaching of melamine from melamine tablewares. Calf thymus (ct) DNA is added to the samples and the extent of DNA damage in vitro is analyzed by Comet assay (also called Single cell gel electrophoresis). The results from comet assay portray significant DNA damage with the treated samples. This is a vigilance study that helps the mundane man to avoid such decorative materials and to voluntarily move to our traditional dining culture.

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References

  1. Bradley EL, Boughtflower V, Smith TL, Speck DR, Castle L (2005) Survey of the migration of melamine and formaldehyde from melamine food contact articles available on the UK market. Food Addit Contam 22(6):597–606

    CAS  Article  Google Scholar 

  2. Bradley EL, Castle L, Day JS, Leak J (2011) Migration of melamine from can coatings cross-linked with melamine-based resins, into food simulants and foods. Food Addict Contam 28(2):243–250

    CAS  Article  Google Scholar 

  3. Bruschweiler ED, Wild P, Khanh C, Huynh A, Savova-Bianchi D, Danuser B, Nancy BH (2016) DNA damage among wood workers assessed with the Comet assay. Environ Health Insights 10:105–112

    Article  Google Scholar 

  4. Firdhouse JM, Lalitha P (2015) Binding properties of biosynthesized gold nanoparticles with calf-thymus DNA in vitro. Int J Biol Chem 9(4):188–197

    CAS  Article  Google Scholar 

  5. https://www.who.int/mediacentre/news/release/2008/pr48/en/

  6. Ingelfinger Julie R (2008) Melamine and the global implications of food contamination. N Engl J Med 359:2745–2748

    CAS  Article  Google Scholar 

  7. Kumaravel TS, Barbara V, Stephen PF, Awadhesh NJ (2009) Comet Assay measurements: a perspective. Cell Biol Toxicol 25(1):53–64

    CAS  Article  Google Scholar 

  8. Partanen H, Vähäkangas K, Woo CSJ, Auriola S, Veid J, Chen Y, Myllynen P, Nezami Eli P (2012) Transplacental transfer of melamine. Placenta 33:60–66

    CAS  Article  Google Scholar 

  9. Sun Y, Rutao L, Zhenxing C, Pengfei Q, Xiaoyan F, Yue M (2010) Spectroscopic investigation on the toxic interaction of melamine with herring sperm DNA. J Biochem Mol Toxicol 24(5):323–329

    CAS  Article  Google Scholar 

  10. Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu JC, Sasaki YF (2000) Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen 35:206–221

    CAS  Article  Google Scholar 

  11. Ullah S, Bustam MA, Naz MN, Tan MY, W.L. (2014) Synthesis and thermal degradation studies of melamine formaldehyde resins. Sci World J 2014:1–6

    Google Scholar 

  12. Wiwanitkit V (2009) Melamine: problems in obstetrics. Arch Gynecol Obstet 280:345–346

    CAS  Article  Google Scholar 

  13. Xiang D, Guoping Z, Kun Z, Li L, Zhike X (2011) Determination of melamine in milk powder based on the fluorescence enhancement of Au nanoparticles. Analyst 136:2837–2844

    CAS  Article  Google Scholar 

  14. Yang VL, Batlle D (2008) Acute renal failure from adulteration of milk with melamine. Sci Worl J 8:974–975

    Article  Google Scholar 

  15. Yang T, Huangfu WG, Wu YL (2011) Melamine residues in eggs of laying hens exposed to melamine-contaminated feed. Poult Sci 90:701–704

    CAS  Article  Google Scholar 

  16. Yurdakok B, Filazi A, Ekici H, Celikm TH, Sireli UT (2014) Melamine in breast milk. Toxicol Res 3:242–246

    CAS  Article  Google Scholar 

  17. Zhang Q, Yang G, Li J, Li W, Zhang B, Zhu W (2011) Melamine induces sperm DNA damage and abnormality, but not genetic toxicity. Regul Toxicol Pharmacol 60:144–150

    CAS  Article  Google Scholar 

  18. Zhou W, Yang H, Jiang Y, Shi H (2010) The Characteristics of immune system changes in children who ingested melamine-contaminated powdered formula in China. Int J Environ Health Res 20(4):289–297

    CAS  Article  Google Scholar 

  19. Zhu H, Kannan K (2018) Distribution profiles of melamine and its derivatives in indoor dust from 12 countries and the implications for human exposure. Environ Sci Technol 52, 12801−12808.

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Acknowledgements

The authors sincerely acknowledge the Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore for providing facilities to carry out the research work. The consumables were procured under the DST-CURIE fund. The small financial assistance (Rs.3000/month) in the form of fellowship by GURUKADAKSHAM TRUST is gratefully acknowledged.

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Correspondence to Lalitha Pottail.

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Chithambharan, A., Pottail, L., Sharma, S.C. et al. FT-IR fingerprinting as an Analytical tool for determination of Melamine leaching from Melamine tablewares and their Biological implications. J Food Sci Technol (2020). https://doi.org/10.1007/s13197-020-04599-9

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Keywords

  • Comet assay
  • Food stimulants
  • FT-IR
  • Melamine
  • Melamine leaching