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Effect of succinylation on the secondary structures, surface, and thermal properties of date palm pollen protein concentrate

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Abstract

The present study was attempted to investigate the effect of succinylation, as chemical modification, on the functionality of male date palm pollen protein concentrate (MDPPPC). Succinylation was applied at two levels, 4 and 8 mol of succinic acid per mole of lysine. 4 M and 8 M were compared to the native MDPPPC. Findings proved that succinylation improved the surface properties of pollen protein including solubility and surfactant activity. Increased solubility of succinylated MDPPPC has been noticed especially in pH superior to pHi. The results from the differential scanning calorimetry showed a significant decrease (P < 0.05) of the denaturation temperature and the heat enthalpy for succinylated MDPPPC. β turn of succinylated MDPPPC increased significantly (P < 0.05) at the expense of β sheet indicating that the protein gained more mobility after succinylation which explains the enhancement of the functional properties and promotes the use of succinylated protein as a techno-functional ingredient.

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Acknowledgment

This work was funded by the Ministry of Higher Education and Scientific Research–Tunisia and Wallonie Bruxelles International (Axis 2, project 9).

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Authors and Affiliations

  1. Laboratory of Analysis Valorization and Food Safety, University of Sfax, National Engineering School of Sfax, Soukra Road, BP W-3038, Sfax, Tunisia

    Haifa Sebii, Sirine Karra, Brahim Bchir, Abir Mokni Ghribi & Souhail Besbes

  2. Gembloux Agro-Bio Tech, Laboratory of Food Science and Formulation, University of Liège, Passage des Déportés 2 B, B-5030, Gembloux, Belgium

    Christophe Blecker

  3. University of Artois, EA 7394, Institut Charles Viollette, F-62300, Lens, France

    Zeineb Nhouchi & Romdhane Karoui

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  1. Haifa Sebii
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  2. Sirine Karra
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  3. Brahim Bchir
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  4. Zeineb Nhouchi
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  5. Abir Mokni Ghribi
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  6. Romdhane Karoui
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  7. Christophe Blecker
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  8. Souhail Besbes
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Correspondence to Haifa Sebii.

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Sebii, H., Karra, S., Bchir, B. et al. Effect of succinylation on the secondary structures, surface, and thermal properties of date palm pollen protein concentrate. J Food Sci Technol 58, 632–640 (2021). https://doi.org/10.1007/s13197-020-04577-1

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  • DOI: https://doi.org/10.1007/s13197-020-04577-1

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