Aminoglycosides belong to a class of antibiotics now widely used in agriculture and veterinary medicine and expected to contaminate food products. In this study, a sensitive lateral flow immunoassay (LFIA) of an aminoglycoside neomycin (NEO) was developed. Two methods of immunochromatographic detection based on various techniques of gold nanoparticles (AuNPs) introduction as a label were compared. It was demonstrated that the indirect labeling (a conjugation of anti-species antibodies with a marker) allowed for an increase in assay sensitivity by 80 times. The test system was characterized by an instrumental limit of detection of 0.1 ng/mL and the cutoff level of 10 ng/mL; the assay duration was 15 min. Specificity only toward NEO was demonstrated. The developed LFIA has been tested to detect NEO in different foodstuffs. It has been demonstrated that 70–119% of NEO (coefficients of variations < 10%) can be determined in milk, turkey meat, honey, and eggs using simple procedures of preliminary sample preparation. Testing the samples showed the coincidence of the results for the developed lateral flow assay and for commercial ELISA kit.
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The work was supported by the Russian Foundation for Basic Research, project 18-58-00038, and the Belarusian Republican Foundation for Fundamental Research, project X18P-060. The authors are grateful to S. M. Pridvorova (Federal Research Center "Fundamentals of Biotechnology") for obtaining electronic microphotographs of AuNPs and D. S. Popravko for developing Fig. 3.
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Hendrickson, O.D., Byzova, N.A., Zvereva, E.A. et al. Sensitive lateral flow immunoassay of an antibiotic neomycin in foodstuffs. J Food Sci Technol (2020). https://doi.org/10.1007/s13197-020-04541-z
- Food safety
- Lateral flow assay